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Protein-Protein Interactions: A Molecular Cloning Manual


by Erica Golemis

List Price: $147.00
Available: Usually ships in 24 hours
Sales Rank: 1086199
Studio: Cold Spring Harbor Laboratory Press
Binding: Paperback
Number Of Pages: 682
Publication Date: December 15, 2001
Publisher: Cold Spring Harbor Laboratory Press


EDITORIAL REVIEWS

Product Description
Fox Chase Cancer Center, Philadelphia, PA. Manual provides a practical guide to critical technologies in the investigation of protein-protein interactions. Reviews conceptual issues and presents techniques for the characterization and manipulation of individual proteins. Softcover, hardcover also available.


CUSTOMER REVIEWS (Average Customer Rating: 2.5 based on 3 reviews)

Revised, Updated, Expanded Second Edition  
This second edition is much changed from the first edition of 2002. Most of the chapters in the earlier have been either extensively revised and brought up to date or replaced with new chapters (the old chapters are still available on a special web site).

The format of the book is the same with forty-six chapters written by the leading researchers in the field. The chapters are grouped into sections as follows:

1. Protein-Protein Interactions in Biological Context
2. Standard Technologies to Probe Protein Interactions
3. Biophysical Approaches to Probe Protein Interactions
4. Novel High-Throughput Approaches to Probe Protein Interactions
5. Interactions of Proteins and Peptides
6. InVivo Imaging of Protein Interactions
7. Genome-wide and Computer-based Analysis of Protein Interactions
8. Design of Novel Interactions and Inhibitors in Drug Discovery

This book is a summary of the state of the art in the field and is significantly improved over the first edition.
January 25, 2006

some good looking protocols - but bad mistakes: useless for a real scientist  
Most of the protocols may be as good as they appear - no doubt about it! Most of the protocols have sure been written by well established scientists with a lot of input into details. But CSHLpress has an extremely good reputation to edit the very best books like the so-called "Maniatis" known for more than two decades as "the" standard in "molecular Cloning". With this respect the first edition of "Protein-protein interactions: A molecular cloning manual" only "copies" the famous word "cloning" on the title of the book and uses (legally) the logo "CSHLpress" to give the impression of a similar standard. But this impression is wrong: simple mistakes in the introduction of a method support strong doubts about how valuable the following protocol could be. This is not a good example of "CSHLpress"'s own extremely high standard. But hopefully most of the methods represent a good and detailed overview which may (or may not) work. I suggest to avoid the first edition and check carefully the 2nd edition soon. There is at least some room for improvement to reach the otherwise often unreached standards of CSHLpress.
September 27, 2005

simple mistakes - appears as no scientist ever checked for mistakes?  
the very first impression of the book is pretty good: it appears to collect many useful and detailed protocols - useful especially for students and attractive for supervisors - and of course libraries. Unfortunately the first impression doesn't hold: very simple (and easily avoidable) mistakes show that neither the authors (or at least some of them) never ever checked for logistic mistakes - therefore it is very questionable if the protocols ever worked the way they were described. There is only little hope for an improvement of the (soon appearing) second editions. It may be worth for students to check the original literature where at least some peer review process could improve sophisticated protocols.
July 20, 2005
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