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PCR Sequencing Protocols (Methods in Molecular Biology)


by Ralph Rapley

List Price: $89.50
Available: Usually ships in 24 hours
Sales Rank: 3918575
Studio: Humana Press
Binding: Spiral-bound
Number Of Pages: 221
Publication Date: January 15, 1996
Publisher: Humana Press


EDITORIAL REVIEWS

Product Description
A superb, comprehensive collection of the most reliable protocols for PCR sequencing-all developed and described by eminent researchers and scientists who use them successfully every day. These protocols deal practically with those problems accompanying the rapid reassociation of amplified complementary strands by showing how sequencing reactions may be effectively modified, as well as complex methods of primer manipulation that enable the affinity purification, or its transcript production, of homogeneous single strands. They also provide all the necessary details of PCR purification, primer labeling, the preparation of sequencing gels, as well as of conventional methods for cloning PCR products into vectors and their subsequent sequencing.
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