| View Larger Image | Human Retrovirus Protocols: Virology and Molecular Biology (Methods in Molecular Biology) | Hardcoverby Tuofu Zhu (Editor)
| List Price: | $145.00 | | | Available: | Usually ships in 3 to 5 weeks |
| | Binding: | Hardcover | | Publisher: | Humana Press | | Edition: | 1st Edition | | Page Count: | 512 Pages | | Publication Date: | May 17, 2005 | | Sales Rank: | 1,034,884st |
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Product Description
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection. |
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