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Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate [An article from: International Biodeterioration & Biodegradation]
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Enzymatic hydrolysis of corncob and ethanol production from cellulosic hydrolysate [An article from: International Biodeterioration & Biodegradation] | Digital

by M. Chen (Author), L. Xia (Author), P. Xue (Author)

List Price: $10.95  
Available:  Available for download now

Binding:  Digital
Publisher:  Elsevier
Page Count:  4 Pages
Publication Date:  March 01, 2007
Sales Rank:  5,775,461th


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Product Description
This digital document is a journal article from International Biodeterioration & Biodegradation, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.Description: Enzymatic hydrolysis of corncob and ethanol fermentation from cellulosic hydrolysate were investigated. After corncob was pretreated by 1% H"2SO"4 at 108^oC for 3h, the cellulosic residue was hydrolyzed by cellulase from Trichoderma reesei ZU-02 and the hydrolysis yield was 67.5%. Poor cellobiase activity in T. reesei cellulase restricted the conversion of cellobiose to glucose, and the accumulation of cellobiose caused severe feedback inhibition to the activities of @b-1,4-endoglucanase and @b-1,4-exoglucanase in cellulase system. Supplementing cellobiase from Aspergillus niger ZU-07 greatly reduced the inhibitory effect caused by cellobiose, and the hydrolysis yield was improved to 83.9% with enhanced cellobiase activity of 6.5CBUg^-^1 substrate. Fed-batch hydrolysis process was started with a batch hydrolysis containing 100gl^-^1 substrate, with cellulosic residue added at 6 and 12h twice to get a final substrate concentration of 200gl^-^1. After 60h of reaction, the reducing sugar concentration reached 116.3gl^-^1 with a hydrolysis yield of 79.5%. Further fermentation of cellulosic hydrolysate containing 95.3gl^-^1 glucose was performed using Saccharomyces cerevisiae 316, and 45.7gl^-^1 ethanol was obtained within 18h. The research results are meaningful in fuel ethanol production from agricultural residue instead of grain starch.
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