| View Larger Image | Rapid high throughput assay for fluorimetric detection of doxorubicin-Application of nucleic acid-dye bioprobe [An article from: Analytica Chimica Acta] | Digitalby Y. Liu (Author), B. Danielsson (Author)
| List Price: | $10.95 | | | Available: | Available for download now |
| | Binding: | Digital | | Publisher: | Elsevier | | Page Count: | 4 Pages | | Publication Date: | March 21, 2007 |
|
EDITORIAL REVIEWS |
Product Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.Description: A double stranded DNA based fluorescence bioprobe for anticancer agent (doxorubicin) detection is described. This method provides a new way for sensitive DNA/drug interaction study by a homogeneous assay. The probe employs the long-wavelength intercalating fluorophore TOTO-3^(R) (TT3). The anticancer agent, doxorubicin, which interacts with the DNA-TT3 complex, was indirectly measured by the decrease in the fluorescence intensity. Various oligonucleotides with different sequences were examined. Doxorubicin has preference for the oligonucleotide 5'AGCACG3'. Enhanced fluorescence observed for the TT3 intercalation with this oligonucleotide makes the DNA-dye complex a suitable bioprobe for doxorubicin detection by competitive assay. A home-built CCD camera setup was applied along with 384 well plate assay format for high throughput fluorescence imaging. The detection limit can be as low as 25ngmL^-^1 with an upper limit of 100@mgmL^-^1. The recovery test with spiked serum sample shows that this method can be a potential routine method for therapeutic drug monitoring (TDM). |
|
![Rapid high throughput assay for fluorimetric detection of doxorubicin-Application of nucleic acid-dye bioprobe [An article from: Analytica Chimica Acta]](http://ecx.images-amazon.com/images/I/415FBN4EPVL._SL160_.jpg)
