| View Larger Image | Pharmacological properties of angiotensin II receptors in cultured rabbit gingival fibroblasts [An article from: Comparative Biochemistry and Physiology, Part C] | Digitalby N. Ohuchi (Author), K. Hayashi (Author), K. Koike (Author), Y. Kizawa (Author), Kusama (Author)
| List Price: | $8.95 | | | Available: | Available for download now |
| | Binding: | Digital | | Publisher: | Elsevier | | Publication Date: | March 01, 2004 |
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Product Description
This digital document is a journal article from Comparative Biochemistry and Physiology, Part C, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.Description: We demonstrated that angiotensin II (Ang II, 10-1000 nM) induced proliferation of cultured rabbit gingival fibroblasts in a concentration-dependent manner. The Ang II-induced proliferation was inhibited by CV-11974 (AT"1 antagonist; 1 @mM) and saralasin (AT"1/AT"2 antagonist; 1 @mM), but not by PD123,319 (AT"2 antagonist; 1 @mM), suggesting that Ang II-induced proliferation was mediated via AT"1 receptors present in and/or on gingival fibroblasts. The results of Western blot analysis indicated the presence of AT"1 and AT"2 receptors in/on the fibroblasts. In a subsequent radioligand binding assay, the binding of [^3H]Ang II to the fibroblasts was specific and saturable with both high- and low-affinity sites. Competition binding experiments indicated that Ang II completely displaced [^3H]Ang II binding, and CV-11974 and PD123,319 maximally displaced up to approximately 63% and 37% of the total binding, respectively. Ang II and CV-11974 completely displaced the [^3H]DuP753 binding but PD123,319 did not, indicating a single population of binding site. These findings demonstrate that gingival fibroblasts contain both AT"1 and AT"2 receptor subtypes for Ang II, and support that Ang II stimulation of AT"1 receptors results in proliferation of the fibroblasts. |
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