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| View Larger Image | Immobilization of uricase on ZnO nanorods for a reagentless uric acid biosensor [An article from: Analytica Chimica Acta] | Digitalby F. Zhang (Author), X. Wang (Author), S. Ai (Author), Z. Sun (Author), Q. Wan (Author), Z. Zhu (Author)
| List Price: | $8.95 | | | Available: | Available for download now |
| | Binding: | Digital | | Publisher: | Elsevier | | Publication Date: | August 16, 2004 |
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Product Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.Description: A reagentless uric acid (UA) biosensor based on uricase immobilized on ZnO nanorods was developed. Direct electrochemistry and thermal stability of immobilized uricase were studied. The ZnO nanorods derived electrode retained the enzyme bioactivity and could enhance the electron transfer between the enzyme and the electrode. This sensor showed a high thermal stability up to 85^oC and an electrocatalytic activity to the oxidation of uric acid without the presence of an electron mediator. The electrocatalytic response showed a linear dependence on the uric acid concentration ranging from 5.0 x 10^-^6 to 1.0 x 10^-^3molL^-^1 with a detection limit of 2.0 x 10^-^6molL^-^1 at 3@s. The apparent K"M^a^p^p value for the uric acid sensor was estimated to be 0.238mM, showing a high affinity. |
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