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| View Larger Image | 'Knock down' of DNA polymerase @b by RNA interference: recapitulation of null phenotype [An article from: DNA Repair] | Digitalby Y.Y. Polosina (Author), T.A. Rosenquist (Author), A.P. Grollman (Author), Mil (Author)
| List Price: | $8.95 | | | Available: | Available for download now |
| | Binding: | Digital | | Publisher: | Elsevier | | Publication Date: | November 02, 2004 |
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Product Description
This digital document is a journal article from DNA Repair, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.Description: DNA polymerase @b (pol @b) is the major DNA polymerase involved in the base excision repair (BER) pathway in mammalian cells and, as a consequence, BER is severely compromised in cells lacking pol @b. Pol @b null (-/-) mouse embryos are not viable and pol @b null cells are hypersensitive to alkylating agents. Using RNA interference (RNAi) technology in mouse cells, we have reduced the pol @b protein and mRNA to undetectable levels. Pol @b knockdown cell lines display a pattern of hypersensitivity to DNA damaging agents similar to that observed in pol @b null cells. Generation of pol @b knock down cells makes it possible to combine the pol @b null phenotype with deficiencies in other DNA repair proteins, thereby helping to elucidate the role of pol @b and its interactions with other proteins in mammalian cells. |
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