| Effects of alcohol on intraovarian nitric oxide synthase and steroidogenic acute regulatory protein in the prepubertal female rhesus monkey *.(Report): ... Journal of Studies on Alcohol and Drugs | Digitalby Vinod K. Srivastava (Author), Gregory A. Dissen (Author), Sergio R. Ojeda (Author), Jill K. Hiney (Author), Michelle D. Pine (Author), W. Les Dees (Author)
| List Price: | $9.95 | | | Available: | Available for download now |
| | Binding: | Digital | | Publisher: | Thomson Gale | | Page Count: | 21 Pages | | Publication Date: | March 01, 2007 |
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This digital document is an article from Journal of Studies on Alcohol and Drugs, published by Thomson Gale on March 1, 2007. The length of the article is 6026 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.From the author: Objective: In addition to affecting hypothalamic-pituitary function, alcohol is a gonadal toxin capable of inhibiting ovarian function and suppressing circulating levels of estradiol ([E.sub.2]) in female rats, rhesus monkeys, and adolescent girls. Both nitric oxide (NO) and steroidogenic acute regulatory protein (STAR) are intraovarian substances that influence steroidogenesis in opposite directions. This study was undertaken to determine whether alcohol exposure affects prepubertal ovanan steroidogenesis in female rhesus monkeys by altering nitric oxide synthase (NOS), STAR, or both. Method: At 20 months of age, monkeys received a single intragastric dose of alcohol (2.4 g/kg) or an equal volume of a saline/sucrose solution daily until they were 36 months old. Blood and ovaries were then collected for assessment of serum hormone levels and tissue gene and protein expression. Results: Alcohol caused depressed levels of serum [E.sub.2] (p < .05) and luteinizing hormone (p < .05) but not follicle-stimulating hormone. Real-time polymerase chain reaction (RT-PCR) assessment of ovarian mRNA encoding the three isoforms (i.e., neuronal [n] NOS, endothelial [e] NOS, and inducible [i] NOS) of NOS revealed that alcohol exposure did not alter gene expression of nNOS but caused increased basal levels of eNOS (p < .05) and iNOS (p < .01) mRNA expression compared with control ovaries. Alcohol also increased expression of eNOS (p < .01) and iNOS (p < .05) proteins. In contrast, ovaries from monkeys exposed to alcohol showed decreased (p < .05) StAR gene expression compared with controis. Conclusions: We showed previously that alcohol exposure during adolescence suppressed [E.sub.2] and delayed development of regular monthly menstruation patterns in rhesus monkeys. The present results suggest that the combined action of alcohol to elevate ovarian NOS and suppress StAR synthesis contributes to these abnormalities.Citation DetailsTitle: Effects of alcohol on intraovarian nitric oxide synthase and steroidogenic acute regulatory protein in the prepubertal female rhesus monkey *.(Report)Author: Vinod K. SrivastavaPublication: Journal of Studies on Alcohol and Drugs (Magazine/Journal)Date: March 1, 2007Publisher: Thomson GaleVolume: 68 Issue: 2 Page: 182(10)Article Type: ReportDistributed by Thomson Gale |
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