Science Current Events | Science News | Brightsurf.com
 

Carnegie Mellon develops bio-mimicry method for preparing and labeling stem cells

May 18, 2016

PITTSBURGH--Researchers led by Carnegie Mellon University Professor of Biological Sciences Chien Ho have developed a new method for preparing mesenchymal stem cells (MSCs) that not only leads to the production of more native stem cells, but also labels them with a FDA approved iron-oxide nanoparticle (Ferumoxytol). The technology could allow researchers to track the cells in vivo using magnetic resonance imaging (MRI) during preclinical and clinical trials. The findings are published by Scientific Reports.

Stem cells, with their ability to regenerate into a multitude of different cell types, show great promise for treating a number of diseases and injuries. Stem cells taken from a patient's own body are of particular interest, due to a decreased chance of rejection. These cells are most commonly harvested from the bone marrow, which contains two types of stem cells, hematopoietic and mesenchymal.

Hematopoietic stem cells can be used to form the different types of blood cells and are used to treat leukemia and multiple myeloma. Mesenchymal stem cells can be used to generate bone, cartilage and fat cells, and have promise for repairing bone and cartilage, damaged heart cells, and treating inflammatory and autoimmune diseases.

More than 360 registered clinical trials are using MSCs, but the results have been mixed, with some patients reacting well and others not responding to the stem cell treatment. To understand why these results can be so variable, researchers need to be able to track the stem cells as they migrate through the body to see if they reach and graft to the appropriate site. To do this, researchers could label the stem cells with a superparamagnetic iron-oxide (SPIO) contrast agent and image the patient using MRI.

Ferumoxytol is the only SPIO nanoparticle that has been approved by the FDA, but researchers have not been able to label MSCs with Ferumoxytol in cell culture (ex vivo) without the help of a transfection agent. Transfection agents are undesirable because they can change the cells' biology and inhibit their effectiveness. Furthermore, researchers have had difficulty culturing the large amount of cells needed for clinical dosing. Current methods also result in cells of different sizes and functionalities. Smaller, round cells are preferable because they show a higher capacity for regeneration and differentiation.

To surmount these problems, Ho and colleagues took advantage of the cell's natural ability to engulf and internalize Ferumoxytol in vivo. Ho's team developed a "bio-mimicry" method to create an environment in a petri dish that is much like the environment found inside the body. His team began by using traditional methods to extract cells from bone marrow, separate the MSCs from the other cells and expand the number of MSCs. His team then devised a new way to culture MSCs by introducing other cells from the bone marrow, mimicking the in-vivo environment. The resulting MSCs retain their optimal size and regeneration capabilities and can internalize Ferumoxytol for cell tracking. Because MSCs are multi-potent, this new methodology can prepare more native cells for applications in cell therapy and regenerative medicine.

###

Additional study authors include Li Liu, Lanya Tseng, Qing Ye and Yijen Wu from Carnegie Mellon's Department of Biological Sciences, and Daniel J. Bain from the Department of Geology and Environmental Science at the University of Pittsburgh. The research was funded by the National Institutes of Health's National Institute of Biomedical Imaging and Bioengineering (EB-001977).

Carnegie Mellon University


Related Mesenchymal Stem Cells Current Events and Mesenchymal Stem Cells News Articles


Zika virus infects human placental macrophages
One of Zika's mysteries is how the virus passes from an infected mother, through the placenta, to a developing fetus.

Harnessing engineered slippery surfaces for tissue repair
Transplanting a preformed dense and coherent sheet of regenerative stem cells directly onto damaged heart, cartilage or bone tissue of ailing patients often is a more promising route to recovery than transplanting the cells just loosely mixed together.

Seaweed offers the solution to transporting stem cells and wound treatment
Publishing in STEM CELLS Translational Medicine Professor Che Connon and Dr Stephen Swioklo describe the low-cost seaweed solution.

Sepsis: Cell therapy to repair muscle long-term impairment
Scientists from the Institut Pasteur, Paris Descartes University, Sainte-Anne Hospital and the CNRS have recently published a paper in Nature Communications in which they reveal mayor players in the severe muscle damage caused by sepsis, or septicemia, which explains why many patients suffer debilitating muscle impairment long-term after recovery.

Samumed announces modulation of Wnt pathway for potential cartilage regeneration
At the American College of Rheumatology (ACR) Annual Meeting, Samumed unveiled groundbreaking pre-clinical and clinical research that demonstrated successful modulation of the Wnt pathway for potential applications in regenerative medicine.

New study reveals how specialized cells help each other survive during times of stress
A team led by scientists from the Florida campus of The Scripps Research Institute (TSRI) and the University of Pittsburgh has shown for the first time how one set of specialized cells survives under stress by manipulating the behavior of key immune system cells.

Department of Defense awards nearly $6 million grant to further bone fracture repair research
A collaborative research team led by scientists at Houston Methodist is one step closer to developing technologies that could help mend broken bones faster.

Cartilage regeneration possibilities may improve with fetal cartilage cell transplantation
The self-repair of injured cartilage is difficult for several reasons, foremost of which is the lack of blood supply to the tissue.

New way to repair nerves: Using exosomes to hijack cell-to-cell communication
Regenerative medicine using stem cells is an increasingly promising approach to treat many types of injury.

Stem cell injections improve diabetic neuropathy in animal models
Diabetic neuropathy (DN) is a condition in which perpetually high blood sugar causes nerve damage, resulting in a myriad of symptoms such as numbness, reduced ability to detect painful stimuli, muscle weakness, pain, and muscle spasms.
More Mesenchymal Stem Cells Current Events and Mesenchymal Stem Cells News Articles

Mesenchymal Stem Cells: Methods and Protocols (Methods in Molecular Biology)

Mesenchymal Stem Cells: Methods and Protocols (Methods in Molecular Biology)
by Massimiliano Gnecchi (Editor)


This volume aims to outline the current status of the Mesenchymal Stem Cells(MSC) field in regenerative medicine and to propose clear and reproducible protocols to better define the identity, function and use of these cells that are today, more than ever, “under the spotlight”. Mesenchymal Stem Cells: Methods and Protocols, Second Edition is organized into four sections. The first guides the reader through a series of state-of-the-art reviews summarizing the use of MSC for the treatment of various diseases. The other three sections are a collection of methodological chapters covering several aspects: isolation and characterization of MSC; expansion of MSC for clinical use; production and characterization of the MSC secretome. Written in the highly successful Methods in Molecular...

Mesenchymal Stem Cells and Skeletal Regeneration

Mesenchymal Stem Cells and Skeletal Regeneration
by Peter Giannoudis (Author), Elena Jones (Author), Xuebin Yang (Author), Dennis Mcgonagle (Author)


This book covers our current understanding of the role of mesenchymal stem cells (MSCs) and other mesenchymal progenitors in skeletal regeneration, encompassing bone, cartilage and whole joint regeneration. The expansion reflects developments in the field to include data on the use of MSCs in drug development, growth factors, scaffolds and biomechanical manipulations for skeletal trauma and diseases, including osteoporosis and arthritis. Written for an audience of clinicians and young researchers who are exposed to MSCs in their work, this work summarizes recent findings pertaining to the definition and characterization of MSCs in skeletal tissues and discusses the mechanisms of their actions in regeneration of bone in vivo. The authors describe recent findings pertaining to the efficacy...

Mesenchymal Stem Cell Derived Exosomes: The Potential for Translational Nanomedicine

Mesenchymal Stem Cell Derived Exosomes: The Potential for Translational Nanomedicine
by Yaoliang Tang (Author), Buddhadeb Dawn (Author)


Mesenchymal stem cell-derived exosomes are at the forefront of research in two of the most high profile and funded scientific areas – cardiovascular research and stem cells. Mesenchymal Stem Cell Derived Exosomes provides insight into the biofunction and molecular mechanisms, practical tools for research, and a look toward the clinical applications of this exciting phenomenon which is emerging as an effective diagnostic. Primarily focused on the cardiovascular applications where there have been the greatest advancements toward the clinic, this is the first compendium for clinical and biomedical researchers who are interested in integrating MSC-derived exosomes as a diagnostic and therapeutic tool.Introduces the MSC-exosome mediated cell-cell communicationCovers the major functional...

Mesenchymal Stem Cells and Immunomodulation (Stem Cell Biology and Regenerative Medicine)

Mesenchymal Stem Cells and Immunomodulation (Stem Cell Biology and Regenerative Medicine)
by Charles J. Malemud (Editor), Eben Alsberg (Editor)


This essential volume explores mesenchymal stem cells (MSCs) and their potential to suppress immune-mediated inflammation. The chapters examine applications in autoimmune diseases such as lupus, rheumatoid arthritis and multiple sclerosis; blood cancers such as leukemia and lymphoma; and reproductive complications, specifically pre-term labor and use of MSCs in vitro and in animal models to discover methods of suppressing the causal inflammatory response. It also further defines the methodologies required to develop research on MSCs in vitro into established preclinical animal models including those which are proven replicas of autoimmunity and pre-term labor, to name but two.
Mesenchymal Stem Cells and Immunomodulation, part of Springer’s Stem Cell Biology and Regenerative Medicine,...

Mesenchymal Stem Cells in Cancer Therapy

Mesenchymal Stem Cells in Cancer Therapy
by Khalid Shah (Author)


With the thorough understanding of stem cell biology and the advent of targeted therapeutics for cancer, stem cell-based therapeutic strategies are being increasingly explored for the treatment of various cancer types. Mesenchymal Stem Cells in Cancer Therapy sheds light on current stem cell based targeted therapies for cancer, by focusing on the application of mesenchymal stem cells (MSC) in various cancers with emphasis on a number of aspects that are critical to the success of future stem cell based therapies for cancer. Sections of this publication are devoted to developing stem cell based therapies for cancer with the main focus on tumoritrophic properties of stem cells, engineering targeted therapeutics, utilization of imaging techniques and the recent combination studies...

Stem Cell Processing (Stem Cells in Clinical Applications)

Stem Cell Processing (Stem Cells in Clinical Applications)
by Phuc Van Pham (Editor)


This invaluable resource delineates procedures for development and use of stem cells in the laboratory and explores the potential for clinical applications. The text discusses mesenchymal stem cell isolation, isolation of adipose derived stem cells, new trends of induced pluripotent stem cells in disease treatment, cord blood banking, future directions of the discussed therapies and much more. The chapters are contributed by preeminent scientists in the field and present a comprehensive picture of stem cell processes, from development in the laboratory to effects and side-effects of clinical application.
Stem Cell Processing and the other books in the Stem Cells in Clinical Applications series, edited by Dr. Phuc Van Pham, is essential reading for scientists, researchers, advanced...

Mesenchymal Stromal Cells: Biology and Clinical Applications (Stem Cell Biology and Regenerative Medicine)

Mesenchymal Stromal Cells: Biology and Clinical Applications (Stem Cell Biology and Regenerative Medicine)
by Peiman Hematti (Editor), Armand Keating (Editor)


Mesenchymal Stromal Cell (MSC) biology has been studied for more than 4 decades and the cells have been investigated for potential clinical applications for more than 15 years. Progress has become exponential over the past decade due mainly to the broad therapeutic potential of these cells.   However, MSC studies have also been subject to controversy and increasing scrutiny as new mechanisms of action are reported and ever-expanding therapeutic applications pursued. In this book, leading authorities from all over the world, who are actively involved in this field, provide state-of-the-art knowledge of the basic biology, translational requirements and latest clinical experience with MSCs.  This cutting edge book is the ideal resource for scientists and clinicians interested in pursuing...

Cell Press Reviews: Stem Cells to Model and Treat Disease (Cell Press Reviews Series)

Cell Press Reviews: Stem Cells to Model and Treat Disease (Cell Press Reviews Series)
by Cell Press (Editor)


Cell Press Reviews: Stem Cells to Model and Treat Disease informs, inspires, and connects stem cell researchers and clinicians at all stages in their careers with timely, comprehensive reviews written by leaders in the field and curated by Cell Press editors. The publication offers a broad view of some of the most compelling topics in stem cell research including: Safety and efficacy of stem cell treatmentsStem-cell-based organ regenerationIntegrating stem cells into personalized drug discoveryUsing pluripotent stem cells for treating neurological diseaseEngineering hematopoietic stem cells for innovative therapies Contributions come from leading voices in the field, including: - Shinya Yamanaka, Recipient of the 2012 Nobel Prize for Physiology or Medicine, Recipient of the 2012...

Clinical applications of mesenchymal stem cells

Clinical applications of mesenchymal stem cells


Mesenchymal stem cells (MSC) have generated a great amount of enthusiasm over the past decade as a novel therapeutic paradigm for a variety of diseases. Currently, MSC based clinical trials have been conducted for at least 12 kinds of pathological conditions, with many completed trials demonstrating the safety and efficacy. This review provides an overview of the recent clinical findings related to MSC therapeutic effects. Roles of MSCs in clinical trials conducted to treat graft-versus-host-disease (GVHD) and cardiovascular diseases are highlighted. Clinical application of MSC are mainly attributed to their important four biological properties- the ability to home to sites of inflammation following tissue injury when injected intravenously; to differentiate into various cell types; to...

Adult Stem Cells: Methods and Protocols (Methods in Molecular Biology)

Adult Stem Cells: Methods and Protocols (Methods in Molecular Biology)
by Paolo Di Nardo (Editor), Sanjiv Dhingra (Editor), Dinender K. Singla (Editor)


This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of cardiomyocytes by gene transfer; isolation of stromal stem cells from adipose tissue; noninvasive assessment of cell fat and biology in transplanted mesenchymal stem cells; and cell-free therapy for organ repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known...

© 2017 BrightSurf.com