Science Current Events | Science News | Brightsurf.com
 

Harnessing engineered slippery surfaces for tissue repair

May 18, 2016

(Boston)-- Transplanting a preformed dense and coherent sheet of regenerative stem cells directly onto damaged heart, cartilage or bone tissue of ailing patients often is a more promising route to recovery than transplanting the cells just loosely mixed together. The challenge in obtaining intact cell sheets, however, lies in releasing them from the substrate they are grown on in the culture dish quickly and without affecting their efficiency. Current methods to detach intact transplantable cell sheets from their growth surfaces in the culture dish require manipulations like a shift in temperature that can impact the efficiency of finicky cell types suitable for specific treatments and be prohibitively expensive for developing new applications.

As reported on 18 May in Scientific Reports, Joanna Aizenberg's team at the Wyss Institute for Biologically Inspired Engineering and the John A. Paulson School of Engineering and Applied Sciences (SEAS) at Harvard has developed a new method that can induce slipperiness on a growth supporting surface at will and sets the stage for the fast, efficient and inexpensive recovery of intact sheets of mesenchymal stem cells (MSCs) with broad potential for regenerative medicine.

Earlier work by Aizenberg was based on the observation that the Nepenthes pitcher plant, in times of rain, can reversibly render its surface slippery enabling it to trap insects. This ability in mind, Aizenberg's team pioneered Slippery Liquid-Infused Porous Surfaces (SLIPS) as a general anti-fouling strategy.

"We leveraged these same basic principles that drove our previously conceived SLIPS, in which a thin oil layer formed on a polymeric matrix repels unwanted substances and organisms including ice, blood, bacteria or crude oil from a surface. In our new study, we use those principles as a 'creative', rather than 'preventive', strategy to generate a switchable surface in which we can induce slipperiness and release at will, at a time when an intact cell sheet with regenerative capacities has formed on the surface," said Aizenberg, Ph.D., who is a Core Faculty member at the Wyss Institute, leader of the Wyss Adaptive Material Technologies platform, and also is the Amy Smith Berylson Professor of Materials Science at SEAS.

As the method's linchpin, the team generated a thin layer of the commonly used, biocompatible polymeric material polydimethylsiloxane (PDMS) in a cell culture dish that was infused with a chemically matched, non-toxic silicone oil to saturation. In addition, the infused polymer was coated with human fibronectin (FN), an extracellular matrix protein that MSCs and other cell types able to repair damaged tissues can adhere to and proliferate on in their natural tissue environments or in culture conditions.

"Once we have grown a near-confluent sheet of cells on top of the oil-infused, FN-coated polymer, we can carefully inject a small amount of oil underneath the cell layer. Because the polymer itself is already saturated, the oil can be freely moved around as a pool to detach the entire sheet including its adherent FN substrate in only about five minutes, much more rapidly than other methods would allow. Using a filter paper, we then are able to transfer the cell sheet to a new surface that in the future could be replaced by target tissues for in vivo regenerative approaches," said Nidhi Juthani, a Visiting Student working with Aizenberg and the first author on the study.

Aizenberg's team, co-led by Wyss Technology Development Fellow Caitlin Howell (now Assistant Professor at the University of Maine), is working to further tailor and automate the procedure. Their goal is to facilitate the engineering of more differentiated cell sheet products such as muscle tissue or cartilage, and enable higher throughput generation and testing of multiple standardized cell sheets in parallel.

"Our technology is highly tunable: by changing the elasticity and geometry of the oil-infused polymer and adding in tissue-specific extracellular matrix proteins and differentiation factors that then become integral to the cell sheet, we may be able to tailor it to the engineering of diverse cell sheets suitable for various regenerative processes. This versatility, low cost and simplicity have not been possible with existing methods," said Aizenberg.

"Joanna's SLIPS technology is continuing to show its value in entirely unexpected ways. This is also an excellent example of how new advances in fundamental materials science can open new paths in biomedical research, and why we strive to promote collaboration across a broad range of disciplines here at the Wyss Institute," said Wyss Institute Founding Director Donald Ingber, M.D., Ph.D., who is also the Judah Folkman Professor of Vascular Biology at Harvard Medical School and the Vascular Biology program at Boston Children's Hospital, and Professor of Bioengineering at SEAS.

Wyss Institute for Biologically Inspired Engineering at Harvard


Related Tissue Repair Current Events and Tissue Repair News Articles


Is aging inevitable? Not necessarily for sea urchins
Sea urchins are remarkable organisms. They can quickly regrow damaged spines and feet.

Duke study uncovers genetic elements that drive regeneration
If you trace our evolutionary tree way back to its roots -- long before the shedding of gills or the development of opposable thumbs -- you will likely find a common ancestor with the amazing ability to regenerate lost body parts.

Glucose-guzzling immune cells may drive coronary artery disease, Stanford study finds
Hyper-aggressive immune cells parked in arterial plaque and bingeing on glucose appear to be major drivers of coronary artery disease, Stanford University School of Medicine investigators have found.

New evidence gives women informed choice in the prolapse surgery debate
New evidence published today highlights benefits and harms of using artificial mesh when compared with tissue repair in the surgical treatment of vaginal prolapse.

Sylvester researchers describe role of STING protein in development of colorectal cancer
A new study published today by researchers at Sylvester Comprehensive Cancer Center at the University of Miami Miller School of Medicine (Sylvester) reports on a key finding about the immune system's response to tumor development following studies on colorectal cancer.

New nanoscopic tools to study ligand-binding of receptors and quantifying two ligand-binding sites while imaging membrane receptors
Signalling processes in organisms are governed by specific extracellular and intracellular interactions and involve hundreds of different functionally highly versatile receptors situated in cell membranes.

Pitt study: Chronic arsenic exposure can impair ability of muscle to heal after injury
Chronic exposure to arsenic can lead to stem cell dysfunction that impairs muscle healing and regeneration, according to an animal study conducted by researchers at the University of Pittsburgh's School of Medicine and Graduate School of Public Health.

Samumed announces modulation of Wnt pathway for potential cartilage regeneration
At the American College of Rheumatology (ACR) Annual Meeting, Samumed unveiled groundbreaking pre-clinical and clinical research that demonstrated successful modulation of the Wnt pathway for potential applications in regenerative medicine.

New drug protects against the deadly effects of nuclear radiation 24 hours after exposure
An interdisciplinary research team led by The University of Texas Medical Branch at Galveston reports a new breakthrough in countering the deadly effects of radiation exposure.

How newts can help osteoarthritis patients
A research team at York has adapted the astonishing capacity of animals such as newts to regenerate lost tissues and organs caused when they have a limb severed.
More Tissue Repair Current Events and Tissue Repair News Articles

Native Tissue Repair for Incontinence and Prolapse

Native Tissue Repair for Incontinence and Prolapse
by Philippe E. Zimmern (Editor), Elise J. B. De (Editor)


This book demonstrates knowledge on tissue-based procedures for stress urinary incontinence and other pelvic floor related topics. As the field shifts away from using synthetic material for repair, this work presents a new perspective on native tissue repair from the opinions and research of the authors.Written by a panel of expert authors in the field, this text provides high quality, focused information that is complimented by illustrations and videos. With established track records the authors illustrate how to perform the procedure vaginally or through open surgery, and inclusion of live surgeries via online video, makes this an invaluable tool for busy surgeons and specialists interested in pelvic floor reconstruction. 
 

Dodge Full-Size Pickups, 1994-2001 (Haynes Repair Manuals)

Dodge Full-Size Pickups, 1994-2001 (Haynes Repair Manuals)
by John Haynes (Author)


Whether the reader has simple maintenance or a complete engine rebuild in mind, he or she can rest assured that there's a Haynes manual for just above every popular domestic and import car, truck, and motorcycle. By conducting complete tear-downs and rebuilds, the staff at Haynes Publishing has discovered all the problems owners will find when repairing or rebuilding their vehicles. By documenting each process with hundreds of illustrations and step-by-step instructions that show the exact order of assembly, Haynes manuals make every step easy to follow.

Introduction To Book Repair: Featuring- Book structure 101, Heat-set Tissue & Japanese Tissue

Introduction To Book Repair: Featuring- Book structure 101, Heat-set Tissue & Japanese Tissue
by Gregory Elden Ware


This book was written to share some of my 40 years of experience in the bookbinding industry. This book will help library directors, managers and staff to preserve some of their collections. Church members will be interested in repairing hymnbooks. Children can be taught this basic structure to help their understanding of bound books. Also, paper repair as a craft for young children can teach them the love and care of other valuable items. Those that are retired and need the convenience of working at home while earning an extra income would enjoy this book. The structure of a hard case bound library book is broken down to its smallest unit and rebuilt. The basic use of Japanese tissue is explored with applied application. The use of heat set tissue with the heat set tool is...

Fibrocytes: New Insights into Tissue Repair And Systemic Fibrosis

Fibrocytes: New Insights into Tissue Repair And Systemic Fibrosis
by Richard Bucala (Author), Richard Bucala (Editor)


Since the discovery of the circulating fibrocyte in 1994 as a collagen-producing cell of the peripheral blood, the physiologic and pathologic role of this unique cell populaton has grown steadily. This pioneering new book provides the first comprehensive review of the role of fibrocytes in wound repair, granuloma formation, antigen presentation, scar formation, and various fibrosing disorders such as interstitial lung disease and nephrogenic systemic fibrosis. It also includes discussions of the recent studies on the molecular signals that influence fibrocyte migration, proliferation, and function in the context of normal physiology and pathology. The chapters are contributed by the leading researchers in the field.

Tissue Repair: Reinforced Scaffolds

Tissue Repair: Reinforced Scaffolds
by Xiaoming Li (Editor)


This book summarizes the effective reinforcement of scaffolds by means of different kinds of fibers and tubes to meet different needs in the context of tissue repair. It covers the fabrication of the reinforced scaffolds, the factors influencing their properties, and their applications for hard and soft tissue repair. Further, it presents a range of concrete examples, case studies and research frontiers, providing readers a better understanding of how the respective fibers or tubes influence the mechanical properties, biodegradability, biocompatibility and bioactivity of scaffolds, and how they fulfill specific medical requirements. As such, the book provides a valuable and informative resource for researchers, technicians and students in the fields of biomaterials, tissue engineering and...

Cardiac Regeneration and Repair: Biomaterials and Tissue Engineering (Woodhead Publishing Series in Biomaterials)

Cardiac Regeneration and Repair: Biomaterials and Tissue Engineering (Woodhead Publishing Series in Biomaterials)
by Ren-Ke Li (Editor), Richard D. Weisel (Editor)


Cardiac Regeneration and Repair, Volume Two reviews the use of biomaterials, alone or combined with cell therapy, in providing tissue-engineered constructs to repair the injured heart and prevent or reverse heart failure. Part one explores the variety of biomaterials available for cardiac repair, including nanomaterials and hydrogels. Further chapters explore the use of biomaterials to enhance stem cell therapy for restoring ventricular function and generating stem cell-modified intravascular stents. Part two focuses on tissue engineering for cardiac repair, including chapters on decellularized biologic scaffolds, synthetic scaffolds, cell sheet engineering, maturation of functional cardiac tissue patches, vascularized engineered tissues for in vivo and in vitro applications, and clinical...

Auto Repair For Dummies

Auto Repair For Dummies
by Deanna Sclar (Author)


Most of us don’t know the first thing about the machines we’re licensed to drive – and this can turn a ticket to freedom into a ticket to trouble. If you’re like most people, you probably tend to drive around until something goes wrong with the car. You then incur the expense of replacing worn and burnt-out parts (or the entire engine) when low-cost, regular maintenance could have kept your wheels turning for a long time. Auto Repair For Dummies is indispensable for anyone who is tired of nodding and smiling at the incomprehensible mutterings of your mechanic, only to end up shelling out money for repairs that you neither fully understand nor always need. This easy-to-understand guide is also for you if you Don't have the vaguest idea of how a car works. Can't identify anything...

Stem Cell Therapy and Tissue Engineering for Cardiovascular Repair: From Basic Research to Clinical Applications

Stem Cell Therapy and Tissue Engineering for Cardiovascular Repair: From Basic Research to Clinical Applications
by Nabil Dib (Editor), Doris A. Taylor (Editor), Edward B. Diethrich (Editor)


In excess of 7 million people worldwide die of coronary heart disease each year.  Only one-third of these heart attack victims recover completely.  The remainder suffer the consequences of myocardial infarction and its ill fated remodeling process, resulting in chronic congestive heart failure.  This malady alone is the leading cause of hospital admissions in the United States. New breakthroughs in stem cell therapy and tissue engineering have promised to reverse this dismal outcome by cardiovascular repair.  World authorities, including scientists and regulatory authorities, have joined in a collaborative effort to present for the reader the first collective review of stem cell therapy for the treatment of cardiovascular disease.  These contributions in basic science, pre-clinical...

The Tissues of the Body. An Introduction to the Study of Anatomy.

The Tissues of the Body. An Introduction to the Study of Anatomy.
by W E Clark (Author)


372 pages, 109 illustrations and coloured plates The part played by the diaphragm in respiration was studied by Traube and Le Gros Clark in 1871. Sir Wilfrid Edward Le Gros Clark (1895-1971)[1] was a British anatomist surgeon, primatologist and palaeoanthropologist, today best remembered for his contribution to the study of human evolution. Le Gros Clark was educated at Blundell's School and subsequently admitted as a medical student to St Thomas' Hospital Medical School in Lambeth. After qualification he immediately joined the Royal Army Medical Corps as a medical officer and was sent to France early in 1918. He caught diphtheria and was sent back to England to recover, following which he spent the remainder of the war as a medical officer at ''No. 8 Stationary Hospital'' at Wimereux...

Tissue Repair in a Model of Type 1 Diabetes: Relevance to Microvascular Blood Flow

Tissue Repair in a Model of Type 1 Diabetes: Relevance to Microvascular Blood Flow
by Maryam Bassirat (Author)


Delayed tissue repair is a serious complication oflong-term diabetes mellitus. Prior to this studythere was no clear evidence as to how diabeteschanges skin microvascular blood flow or how thesechanges influence tissue repair processes in earlydiabetes. The research undertaken within this projectaimed to investigate the factors that are known tocause long-term vascular complications of diabetesand how these factors contribute to early changes inskin microvascular blood flow. The treatmentprotocols used in this work showed that the action ofthese factors is reversible in the early stages ofdiabetes. These same treatments were also effectivein improving the repair processes in our model ofdiabetes. This research raised the possibility thatearly intervention could prevent...

© 2017 BrightSurf.com