Science Current Events | Science News | Brightsurf.com
 

Mouse study links heart regeneration to telomere length

May 31, 2016

Researchers at the Spanish National Center for Cardiovascular Research have discovered that the ends of heart muscle cell chromosomes rapidly erode after birth, limiting the cells' ability to proliferate and replace damaged heart tissue. The study, "Postnatal telomere dysfunction induces cardiomyocyte cell-cycle arrest through p21 activation," which will be published online May 30, 2016 in The Journal of Cell Biology, suggests potential new interventions to boost the heart's capacity to repair itself after a heart attack.

Newborn babies can repair injured myocardium, but, in adults, heart attacks cause permanent damage, often leading to heart failure and death. Newborn mice can also regenerate damaged heart tissue. Their heart muscle cells, or cardiomyocytes, can proliferate and repair the heart in the first week after birth, but this regenerative capacity is lost as the mice grow older and the majority of their cardiomyocytes withdraw from the cell cycle.

Ignacio Flores and colleagues at the Spanish National Center for Cardiovascular Research (CNIC) in Madrid wondered whether the cause of this cell cycle arrest might involve telomeres, repetitive DNA sequences that protect the ends of chromosomes. If telomeres grow too short--due, for example, to a loss of the telomere-extending telomerase enzyme--cells can mistake chromosome ends for segments of damaged DNA, leading to the activation of a checkpoint that arrests the cell cycle.

Flores and colleagues therefore examined the length of telomeres in newborn mouse cardiomyocytes and found that the telomeres rapidly eroded in the first week after birth. This erosion coincided with a decrease in telomerase expression and was accompanied by the activation of the DNA damage response and a cell cycle inhibitor called p21.

Telomerase-deficient mice have shorter telomeres than wild-type animals, and, the researchers discovered, their cardiomyocytes already begin to stop proliferating one day after birth. When Flores and colleagues injured the hearts of one-day-old mice, telomerase-deficient cardiomyocytes failed to proliferate or regenerate the injured myocardium. In contrast, wild-type cardiomyocytes were able to proliferate and replace the damaged tissue.

They also found that knocking out the cell cycle inhibitor p21 extended the regenerative capacity of cardiomyocytes, allowing one-week-old p21-deficient mice to repair damaged cardiac tissue much more effectively than week-old wild-type animals.

Maintaining the length of cardiomyocyte telomeres might therefore boost the regenerative capacity of adult cells, improving the recovery of cardiac tissue following a heart attack. "We are now developing telomerase overexpression mouse models to see if we can extend the regenerative window," says Flores.

Rockefeller University Press


Related Cardiomyocytes Current Events and Cardiomyocytes News Articles


Testing the power of stem cell-derived heart muscle cells
With every heart attack, billions of cardiomyocytes, the contracting heart muscle cells that make the heart pump time after time over a lifetime, are lost which can lead to heart failure or injury.

Carnegie Mellon team develops targeted photosensitizer for cell manipulation
Researchers led by Carnegie Mellon University's Molecular and Biosensor Imaging Center (MBIC) Director Marcel Bruchez have re-engineered a fluorescent probe into a powerful optogenetic photosensitizer that can be used to manipulate cells.

Hearts build new muscle with this simple protein patch
An international team of researchers has identified a protein that helps heart muscle cells regenerate after a heart attack.

Molecular switch that promotes heart cell maturation discovered
A molecular switch that seems to be essential for embryonic heart cells to grow into more mature, adult-like heart cells has been discovered.

Gene therapy clips out heart failure causing gene mutations
Gene therapy can clip out genetic material linked to heart failure and replace it with the normal gene in human cardiac cells, according to a study led by researchers from the Cardiovascular Research Center at Icahn School of Medicine at Mount Sinai. The study is published in the April 29 edition of Nature Communications.

Boston Children's Hospital study reveals first 6 months best for stimulating heart growth
In a recent issue of Science Translational Medicine, Brian Polizzotti, PhD, Bernhard Kuhn, MD, Sangita Choudhury, PhD, and colleagues affiliated with the Boston Children's Hospital's Translational Research Center report that the optimal window of time to stimulate heart muscle cell regeneration (cardiomyocyte proliferation) in humans is the first six months of life.

Oil or fat?
Olive oil is universally considered a much healthier alternative to meat fat. Plant-derived oils (such as olive oil, canola oil, and vegetable oil) largely consist of unsaturated fatty acids, whereas animal fat is richer in the saturated ones.

Fighting tuberculosis using the body's natural anti-microbial processes
A new approach to combatting tuberculosis would take advantage of a complex, natural process called autophagy that the human body uses to recycle nutrients, remove damaged cell components, eliminate invading bacteria, and respond to inflammation.

Heart cells regenerated in mice
When a heart attack strikes, heart muscle cells die and scar tissue forms, paving the way for heart failure. Cardiovascular diseases are a major cause of death worldwide, in part because the cells in our most vital organ do not get renewed.

MIPT researchers grow cardiac tissue on 'spider silk' substrate
Genetically engineered fibers of the protein spidroin, which is the construction material for spider webs, has proven to be a perfect substrate for cultivating heart tissue cells, MIPT researchers found. They discuss their findings in an article that has recently come out in the journal PLOS ONE.
More Cardiomyocytes Current Events and Cardiomyocytes News Articles

Cardiomyocytes: Methods and Protocols (Methods in Molecular Biology)

Cardiomyocytes: Methods and Protocols (Methods in Molecular Biology)
by Gary R. Skuse (Editor), Maureen C. Ferran (Editor)


This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on...

Cardiomyocytes – Active Players in Cardiac Disease

Cardiomyocytes – Active Players in Cardiac Disease
by Klaus-Dieter Schlüter (Editor)


This book summarizes our current understanding about the biology and patho-biology of cardiomyocytes and depicts common techniques for the study of these cells. The book is divided into two parts; the first part provides insight into role and function of cardiomyocytes under normal conditions and describes embryogenesis and differentiation, in the second part the role of cardiomyocytes in aging and disease is discussed and cellular responses under stress conditions illustrated. Cardiomyocytes represent the main mass of the heart, and cellular malfunction directly modifies heart function leading to subsequent heart failure. As such, cardiomyocytes are causative involved in the main reasons of heart failure, such as post-infarct remodeling, hypertensive heart disease, idiopathic heart...

Cardiomyocytes: Webster's Timeline History, 1977 - 2007

Cardiomyocytes: Webster's Timeline History, 1977 - 2007
by Icon Group International (Author)


Webster's bibliographic and event-based timelines are comprehensive in scope, covering virtually all topics, geographic locations and people. They do so from a linguistic point of view, and in the case of this book, the focus is on "Cardiomyocytes," including when used in literature (e.g. all authors that might have Cardiomyocytes in their name). As such, this book represents the largest compilation of timeline events associated with Cardiomyocytes when it is used in proper noun form. Webster's timelines cover bibliographic citations, patented inventions, as well as non-conventional and alternative meanings which capture ambiguities in usage. These furthermore cover all parts of speech (possessive, institutional usage, geographic usage) and contexts, including pop culture, the arts,...

Cardiomyocyte: Webster's Timeline History, 1982 - 2007

Cardiomyocyte: Webster's Timeline History, 1982 - 2007
by Icon Group International (Author)


Webster's bibliographic and event-based timelines are comprehensive in scope, covering virtually all topics, geographic locations and people. They do so from a linguistic point of view, and in the case of this book, the focus is on "Cardiomyocyte," including when used in literature (e.g. all authors that might have Cardiomyocyte in their name). As such, this book represents the largest compilation of timeline events associated with Cardiomyocyte when it is used in proper noun form. Webster's timelines cover bibliographic citations, patented inventions, as well as non-conventional and alternative meanings which capture ambiguities in usage. These furthermore cover all parts of speech (possessive, institutional usage, geographic usage) and contexts, including pop culture, the arts, social...

Principles of Gender-Specific Medicine: Gender in the Genomic Era

Principles of Gender-Specific Medicine: Gender in the Genomic Era
by Academic Press


The announcement that we had decoded the human genome in 2000 ushered in a new and unique era in biomedical research and clinical medicine. This Third Edition of Principles of Gender-Specific Medicine focuses, as in the past two editions, on the essentials of sexual dimorphism in human physiology and pathophysiology, but emphasizes the latest information about molecular biology and genomic science in a variety of disciplines. Thus, this edition is a departure from the previous two; the editor solicited individual manuscripts from innovative scientists in a variety of fields rather than the traditional arrangement of sections devoted to the various subspecialties of medicine edited by section chiefs. Wherever it was available, these authors incorporated the latest information about the...

Cardiomyocytes: Methods and Protocols (Methods in Molecular Biology) (2015-04-03)

Cardiomyocytes: Methods and Protocols (Methods in Molecular Biology) (2015-04-03)
by unknown (Author)




Cardioskeletal Myopathies in Children and Young Adults

Cardioskeletal Myopathies in Children and Young Adults
by John Lynn Jefferies (Editor), Burns Blaxall (Editor), Jeffrey Towbin MD (Editor), Jeffrey Robbins (Editor)


Cardioskeletal Myopathies in Children and Young Adults focuses on plaques that kill people in their 40’s-50’s and the way they start to form in young adulthood. The Annals of Family Medicine report that approximately half of young adults have at least one cardiovascular disease risk factor (Mar 2010), and an increase in cardiovascular mortality rates in young adults was substantiated in a study at Northwestern Medicine (Nov 2011). Given the increasing recognition of genetic triggers behind all types of cardiovascular disease, and the growing population of young adults with primary or acquired myocardial disease, the need has arisen for a reference that offers a comprehensive approach to the understanding of basic, translational, and clinical aspects of specific muscle diseases while...

Heart Cells Made Easy: The Heart Under a Microscope (Cardiology Made Easy Book 3)

Heart Cells Made Easy: The Heart Under a Microscope (Cardiology Made Easy Book 3)
by Too Much Information Publishing


A fantastically simple and illustrated explanations of heart cells. This book dares to make some of the most complex concepts simple and almost enjoyable at times. If you want to know how heart cells work but you do not want to get a headache then this is the book for you. Hand on my own heart, I wish I had this book when I was learning.

FUNCTIONAL ROLE OF NFAT IN VENTRICULAR CARDIOMYOCYTES OF RAT: Analysis of NFAT in cardiovascular disfunction

FUNCTIONAL ROLE OF NFAT IN VENTRICULAR CARDIOMYOCYTES OF RAT: Analysis of NFAT in cardiovascular disfunction
by Attia Anwar (Author)


Catecholamines contribute to the adaption of heart to pressure overload via stimulation of ?- or ? adrenoceptors. While mechanisms resulting in hypertrophic growth of myocytes after adrenoceptor- stimulation are established, the functional consequences on cardiomyocyte contractility or hypertrophy are unknown. The present study investigates whether ?- or ?-adrenoceptor- stimulation by phenylephrine (PE) or isoprenaline (ISO) over 24 h modifies cell shortening in ventricular cardiomyocytes of rat. The calcineurin/NFAT pathway was identified, since addition of BAPTA, cyclosporine or NFAT-decoy oligonucleotides reduced SERCA2A expression in presence of PE as regulators of SERCA2A expression. Inhibition of SERCA up regulation under PE by NFAT decoy oligonucleotides reduced cell...

Calcium Handling in hiPSC-Derived Cardiomyocytes (SpringerBriefs in Stem Cells)

Calcium Handling in hiPSC-Derived Cardiomyocytes (SpringerBriefs in Stem Cells)
by Lee Yee-Ki (Author), Siu Chung-Wah (Author)


Calcium is crucial in governing contractile activities of myofilaments in cardiomyocytes, any defeats in calcium homeostasis of the cells would adversely affect heart pumping action. The characterization of calcium handling properties in human induced pluripotent stem cell-derived cardiomyocytes (iPS-CMCs) is of significant interest and pertinent to the stem cell and cardiac regenerative field because of their potential patient-specific therapeutic use.

© 2017 BrightSurf.com