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Cocktails ameliorate celiac disease

November 28, 2002

The University Rovira i Virgili (Spain), the company Trace Biotech AG (Braunschweig), the Institut für Mikrotechnik Mainz GmbH (IMM, Mainz), and seven other European partners are jointly developing a biosensor for the detection of gluten in food. The goal of the ambitious project is to manufacture a disposable microsystem with integrated modules for a standardised extraction and analysis of gluten in food samples. The system, for the first time, will permit patients suffering from celiac disease (gluten sensitive enteropathy) to conduct an on-the-spot analysis of fresh, cooked, or industrially processed food with the aid of a screening procedure. The project is funded with more than 3 Mio Euro by the â€žQuality of Life" program of the European Commission over a period of three years.

The microsystem relies on a complicated serological test procedure (Enzyme Linked ImmunoSorbent Assay = ELISA), which is based on a modified antibody cocktail. Analysis by optical, electrochemical, or gravimetrical procedures will allow to identify the toxic molecule structures of gluten within a few minutes. IMM experts are responsible for the development of the microfluidic structures. The miniaturised disposable test-kit will be a low-cost tool suited for the mass market.

To date, there are no standardised sensor systems known that can reliably detect traces of gluten in the range of millionths of grams. Test results are frequently invalidated by a lack of tags (epitopes) in the gluten molecule, unknown genetic sequences responsible for toxicity, insufficient methods of extracting food samples, and biochemical interactions. Additionally, even the tiniest contamination during the production of food declared to be free of gluten can cause a considerable risk for patients. In order to prevent possible problems with the application in an early stage, the project involves celiac disease patients in the development process: by accompanying surveys their evaluation will pave the way to serial production.

Institut - Mikrotechnik Mainz IMM GmbH

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