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Minimal cocktail for growing human embryonic stem cells established
March 28, 2006Researchers at Yale have established the minimal nutritional requirements for growing and maintaining human embryonic stem cells, a recipe that is critical for clinical application and for developmental studies, according to an early online report this week in Proceedings of the National Academy of Sciences.
Human embryonic stem cells (hESCs) divide continuously over many generations and have the potential to differentiate into many different cell types. For hESCs to be useful in clinic, the nutrient mix they are grown in must be free of components that may contain toxins, viruses, or materials that might cause an immune response.
Led by Michael Snyder, professor of molecular, cellular and developmental biology, his team has documented a simple mix they call hESC cocktail, or HESCO, that contains only purified recombinant, chemically-synthesized, or purified human factors to support the cell growth.
The researchers based success of the recipe on how well the hESCs were able to preserve their growth characteristics and stem cell markers. To be successful, the cocktail also had to maintain normal cell chromosome profiles, or karyotypes, in the cells and fully support the ability of the cells to differentiate.
"Use of a minimal medium, that is sufficient for the embryonic stem cell growth is expected to make clinical application possible and facilitate developmental studies," according to Jean Lu, a post-doctoral associate and first author on the paper. "Cells incubated in HESCO are easy to grow in an undifferentiated state and can be readily induced to differentiate into all the three basic cell lineages."
The final cocktail for HESCO, that actively support hESC self-renewal, contains the growth factor Wnt3, basic fibroblast growth factor, insulin, transferrin, the B-cell activating factor April/BAFF, cholesterol, and albumin.
Yale University
The researchers based success of the recipe on how well the hESCs were able to preserve their growth characteristics and stem cell markers. To be successful, the cocktail also had to maintain normal cell chromosome profiles, or karyotypes, in the cells and fully support the ability of the cells to differentiate.
"Use of a minimal medium, that is sufficient for the embryonic stem cell growth is expected to make clinical application possible and facilitate developmental studies," according to Jean Lu, a post-doctoral associate and first author on the paper. "Cells incubated in HESCO are easy to grow in an undifferentiated state and can be readily induced to differentiate into all the three basic cell lineages."
The final cocktail for HESCO, that actively support hESC self-renewal, contains the growth factor Wnt3, basic fibroblast growth factor, insulin, transferrin, the B-cell activating factor April/BAFF, cholesterol, and albumin.
Yale University
Human Embryonic Stem Cells Current Events and Human Embryonic Stem Cells News RSSFirst reconstitution of an epidermis from human embryonic stem cells
Stem cell research is making great strides. This is yet again illustrated by a study carried out by the I-STEM* Institute (I-STEM/ Inserm UEVE U861/AFM), published in the Lancet on 21 November 2009. The I-STEM team, directed by Marc Peschanski has just succeeded in recreating a whole epidermis from human embryonic stem cells.
UCI embryonic stem cell therapy restores walking ability in rats with neck injuries
The first human embryonic stem cell treatment approved by the FDA for human testing has been shown to restore limb function in rats with neck spinal cord injuries - a finding that could expand the clinical trial to include people with cervical damage.
NIH-funded researchers transform embryonic stem cells into human germ cells
Researchers funded in part by the National Institutes of Health have discovered how to transform human embryonic stem cells into germ cells, the embryonic cells that ultimately give rise to sperm and eggs.
Endocrine Society calls for expanded scope and funding for stem cell research
Stem cell research holds great promise for the treatment of millions of Americans with debilitating and possibly fatal diseases.
Small mechanical forces have big impact on embryonic stem cells
Applying a small mechanical force to embryonic stem cells could be a new way of coaxing them into a specific direction of differentiation, researchers at the University of Illinois report. Applications for force-directed cell differentiation include therapeutic cloning and regenerative medicine.
Fate Therapeutics announces creation of small molecule platform for commercial-scale reprogramming
Fate Therapeutics, Inc. announced today the generation of human induced-pluripotent stem cells (iPSCs) using a combination of small molecules that significantly improves the speed and efficiency of reprogramming.
Stem cell success points to way to regenerate parathyroid glands
An early laboratory success is taking University of Michigan researchers a step closer to parathyroid gland transplants that could one day prevent a currently untreatable form of bone loss associated with thyroid surgery.
Technique enables efficient gene splicing in human embryonic stem cells
A novel technique allows researchers to efficiently and precisely modify or introduce genes into the genomes of human embryonic stem cells (ESCs) and induced pluripotent stem (iPS) cells, according to Whitehead scientists.
What makes stem cells tick?
Investigators at the Burnham Institute for Medical Research (Burnham) and The Scripps Research Institute (TSRI) have made the first comparative, large-scale phosphoproteomic analysis of human embryonic stem cells (hESCs) and their differentiated derivatives.
Stem cell research: From molecular physiology to therapeutic applications
Stem cell research promises remedies to many devastating diseases that are currently incurable, ranging from diabetes and Parkinson's disease to paralysis.
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