 |
|
Core needle biopsy gives an accurate picture of gene expression in whole tumor
August 21, 2006The gene expression profile detected in the core needle biopsy of a breast tumour is representative of gene expression in the whole tumour. A study published today in the open access journal Breast Cancer Research confirms the reliability of core needle biopsy as a tool in breast cancer diagnosis and prognosis. The study also shows that the gene expression profile of a core needle biopsy might be more accurate than the profile of a surgical sample taken from the same tumour, after the biopsy was carried out. According to the study results, the biopsy procedure seems to trigger the expression of genes involved in wound healing as well as tumour invasion and metastasis, thus modifying the gene expression profile of subsequent surgical samples.
Rosanna Zanetti-Dällenbach from the Women's University Hospital in Basel, Switzerland and colleagues from Stiftung Tumorbank, OncoScore AG and University Hospital in Basel, analysed the gene expression profile of core needle biopsies taken from 22 women diagnosed with breast cancer. For each woman, they compared the biopsy expression profile with the expression profile of a surgical sample taken from the tumour subsequently to the core needle biopsy. Zanetti-Dällenbach et al. quantified the expression of 60 genes known to be involved in breast tumour development using a technique called reverse polymerase chain reaction (PCR). Zanetti-Dällenbach et al. also analysed the gene expression profiles of surgical samples taken from the breast tumours of 317 patients who did not undergo a core needle biopsy.
The results of Zanetti-Dällenbach et al.'s study show that the gene expression levels of the core biopsy and the surgical sample are identical for most women. But the authors find that the expression of four specific genes is significantly increased in the surgical samples compared to the core needle biopsies. In the group of women who did not get a core needle biopsy, however, the expression of these four genes is not increased. These four genes are involved in inflammation and wound repair as well as tumour invasion and metastasis. The authors conclude that their expression must therefore be modified by the core needle biopsy procedure itself. They warn that care should be taken when interpreting the gene expression profile of a surgical sample carried out following a core biopsy. Although the tumor's aggressiveness has not changed, the modified profile in surgical samples obtained after core needle biopsy might influence data interpretation with respect to prediction of risk assessment and treatment response. The core needle biopsy sample may give a cleaner, more accurate and more representative profile of the levels of gene expression in the tumour.
BioMed Central
BioMed Central
Gene Expression Current Events and Gene Expression News RSSNew research into the mechanisms of gene regulation
A team led by Penn State's Ross Hardison, T. Ming Chu Professor of Biochemistry and Molecular Biology, has taken a large step toward unraveling how regulatory proteins control the production of gene products during development and growth.
The Protein Srebp2 Drives Cholesterol Formation in Prion-Infected Neuronal Cells Which May Promote Prion-Dependent Diseases
The regulating protein Srebp2 drives cholesterol formation, which prions need for their propagation, in prion-infected neuronal cells.
Study reveals why certain drug combinations backfire
Combination drug therapy has become a staple for treating many infections. For instance, doctors treat extensively drug resistant forms of tuberculosis with one drug that breaks down the pathogen's protective barriers and opens the door for another to deliver the deathblow.
Penn Study Provides First Clear Idea of How Rare Bone Disease Progresses
An international team of scientists, led by researchers at the University of Pennsylvania School of Medicine, is taking the first step in developing a treatment for a rare genetic disorder called fibrodysplasia ossificans progressiva (FOP), in which the body's skeletal muscles and soft connective tissue turns to bone, immobilizing patients over a lifetime with a second skeleton.
Why can't chimps speak?
If humans are genetically related to chimps, why did our brains develop the innate ability for language and speech while theirs did not?
Treatment to improve degenerating muscle gains strength
A study appearing in Science Translational Medicine puts scientists one step closer to clinical trials to test a gene delivery strategy to improve muscle mass and function in patients with certain degenerative muscle disorders.
BUSM researchers show dieters can experience neurobiological similarities of drug addicts
Researchers from Boston University School of Medicine (BUSM) have shown that intermittent access to foods rich in fat and sugar induces changes in the brain which are comparable to those observed in drug dependence.
FDA approved leukemia drugs shows promise in ovarian cancer cells
The drug Sprycel, approved for use by the U.S. Food and Drug Administration in patients with chronic myeloid leukemia, significantly inhibited the growth and invasiveness of ovarian cancer cells and also promoted their death, a study by researchers with UCLA's Jonsson Comprehensive Cancer Center found.
Deciphering the regulatory code
Embryonic development is like a well-organised building project, with the embryo's DNA serving as the blueprint from which all construction details are derived.
Experimental agent reduces breast cancer metastasis to bone
Researchers have reduced breast cancer metastasis to bone using an experimental agent to inhibit ROCK, a protein that was found to be over-expressed in metastatic breast cancer.
More Gene Expression Current Events and Gene Expression News Articles
 |
Regulation of Gene Expression by Gary H. Perdew (Author), Jack P. Vanden Heuvel (Author), Jeffrey M. Peters (Author) Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activity of a specific cell protein. Each of three sections explores mechanisms of gene regulation and expression, and presents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining target genes for a given transcription copy. Part II outlines the methods for determining how proteins can regulate each other by mediating... |
 |
Gene Expression and Regulation by Jun Ma (Author) This book offers a comprehensive look into the science of gene expression and regulation. Focusing on topics such as actions of nuclear receptors, RNA processing, and DNA methylation and imprinting, "Gene Expression and Regulation" is edited by a leading biologist and includes contributions by experts in the field. Presented in the following five sections, this book covers a full spectrum of topics: The History; The Machinery; The Regulators; The Genome; and Special Topics. The Machinery section covers the transcriptional apparatus and general transcription factors. The Regulators section examines selected gene-specific transcription factors important to regulating gene expression. The Genome section covers issues relevant to the behavior of the genome in relation to gene regulation. The... |
 |
The Psychobiology of Gene Expression by Ernest L. Rossi (Author) Outlines the relationship between genes and human experience. The understandings of gene expression emerging from the Human Genome Project are setting the stage for a profound expansion of our understanding of life. We are just now beginning to learn how the brain, body, and genes interact in everyday life. Here, Ernest Rossi introduces the new science of psychosocial genomics and explores how it will profoundly change our understanding of the pathways of communication among mind, body, and spirit. |
 |
Gene Regulation (BIOS Advanced Text) by David Latchman (Author) Gene regulation is an essential process in the development and maintenance of a healthy body, and as such is a central focus in both basic science and medical research. Gene Regulation, Fifth Edition, provides the student with a clear, up-to-date description of gene regulation in eukaryotes, distilling the vast and complex primary literature into a concise overview. For this fifth edition, in addition to extensive updating of existing material, sections on large-scale methodologies have been expanded, and a new section included on regulation by small interfering RNAs. More detail has been added on the role of multi-protein complexes in transcriptional activation and the discussion of the regulation of transcription factor activity by specific modifications to include acetylation... |
 |
Microarray: Gene Expression Analysis by Virendra Gomase (Author) Microarray is an authoritative work whose goal is to illuminate the crucial role of research methodology in the life sciences. Microarray book will adopt an inclusive editorial approach, encompassing fundamental and blue-sky science and have more immediate medical or commercial applications. The scope and structure of the work will reflect the multidimensional character of drug design, focusing in particular on the fundamental science of biological structures and systems, the use of chemical and biological techniques to elucidate that science, and the applications of this knowledge in areas as diverse as drug discovery. This book contains practical examples, theoretical approaches, a large number of examples and references. Complete theory related to drug design has been covered for the... |
 |
Cap-analysis Gene Expression (Cage): Genome-scale Promoter Identification and Association With Expression Profile and Regulatory Networks by Piero Carninci (Author) The output of eukaryotic genomes is much more complex than expected. Genes produce different variants of RNAs from multiple promoters. One of the ultimate targets of biological analysis is to establish a relationship between the messenger RNAs that are transcribed from the genome and the genomic regions that control their expression - the promoters - in order to decipher the networks that regulate gene expression and the transcription factors that act as master regulators of transcriptional control. Novel technologies have recently appeared that allow deciphering of transcriptional network, based on the identification of the starting site of gene transcription, with the simultaneous measurement of expression level and identification of the promoter elements.These tagging technologies... |
 |
Gene Expression by Seed Media Group A scientific blog interested in evolutionary biology, with a particular focus on evolutionary, population and quantitative genetics. Kindle blogs are fully downloaded onto your Kindle so you can read them even when you're not wirelessly connected. And unlike RSS readers which often only provide headlines, blogs on Kindle contain full text content and images, and are updated wirelessly throughout the day. |
 |
Regulation of Gene Expression by Small RNAs by Rajesh K. Gaur (Editor), John J. Rossi (Editor) New Findings Revolutionize Concepts of Gene Function Endogenous small RNAs have been found in various organisms, including humans, mice, flies, worms, fungi, and bacteria. Furthermore, it’s been shown that microRNAs acting as cellular rheostats have the ability to modulate gene expression. In higher eukaryotes, microRNAs may regulate as much as 50 percent of gene expression. Regulation of Gene Expression by Small RNAs brings together the pioneering work of researchers who discuss their work involving a wide variety of small RNA regulatory pathways in organisms ranging from bacteria to humans. In addition to exploring the biogenesis and processing of these regulatory RNAs, they also consider the functional importance of these pathways in host organisms. Assisting... |
|
Techniques in Genetic Engineering 5: Expression of Cloned Genes [VHS] Starring: Tim Harris | |
 |
Celestial Plea by Gene Newton |
| © 2009 BrightSurf.com |