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CSH Protocols publishes cutting-edge methods for analyzing complex molecular interactions
October 05, 2006With the genomes of hundreds of organisms now catalogued, one of the next major challenges is to identify proteins and their interactions. The current issue of CSH Protocols, published online (www.cshprotocols.org), features two freely available, cutting-edge methods that address this challenge.
Elucidation of protein-DNA interactions has been a tedious process, limited to one protein-interaction partner at a time. But the advent of high-throughput, genomics-based technologies has enabled scientists to build large-scale maps of protein-DNA and protein-protein interactions. These methods-released in CSH Protocols-will be useful to researchers seeking to map these interactions and better understand fundamental biological processes.
Another technical challenge facing researchers is that in a typical cell, proteins are present in relatively small quantities. The second free method in this release of CSH Protocols is "MuDPIT" (for Multidimensional Protein Identification Technology), a means of identifying rare proteins in complex mixtures. This method combines advanced protein identification technologies with computational analysis, and has already been successfully employed in characterizing interacting proteins in yeast.
These methods are among more than 40 new peer-reviewed protocols added to the CSH Protocols database this month. Launched in May of 2006, CSH Protocols now includes more than 700 protocols spanning many fields in cell and molecular biology.
Cold Spring Harbor Laboratory
Molecular Interaction Current Events and Molecular Interaction News RSSScientists create metal that pumps liquid uphill
In nature, trees pull vast amounts of water from their roots up to their leaves hundreds of feet above the ground through capillary action, but now scientists at the University of Rochester have created a simple slab of metal that lifts liquid using the same principle-but does so at a speed that would make nature envious.
Hopkins researchers piece together gene 'network' linked to schizophrenia
Reporting this week in the Archives of General Psychiatry, researchers at the Johns Hopkins University School of Medicine have uncovered for the first time molecular circuitry associated with schizophrenia that links three previously known, yet unrelated proteins.
Researchers explain odd oxygen bonding under pressure
Oxygen, the third most abundant element in the cosmos and essential to life on Earth, changes its forms dramatically under pressure transforming to a solid with spectacular colors. Eventually it becomes metallic and a superconductor.
Case Western Reserve University School of Medicine researchers have evidence a vaccine against malaria will reduce infection and disease rates
Today, researchers at the Case Western Reserve University School of Medicine's Center for Global Health & Diseases published data potentially having a strong effect on the three billion people exposed to malaria every year.
Minimum information standards -- all for 1 and 1 for all
Three papers published by EMBL scientists and their collaborators will make it much easier to share and compare information from large-scale proteomics data. The papers are published in Nature Biotechnology on 8th and 26th August.
Slick and Springy: Brown Research Reveals Protein's Role in Joints
Synovial fluid is slime with a serious purpose: Protecting shoulders, hips and other joints from wear, reducing the likelihood of injuries and arthritis.
UBC researcher finds new way to treat devastating fungal infections
Devastating blood-borne fungal infections that can be lethal for HIV/AIDS, cancer, and organ transplant patients may be treated more successfully, thanks to a new drug delivery method developed by researchers at the University of British Columbia in Vancouver.
New NIAID program aims to model immune responses and key infectious diseases
A new program at the National Institute of Allergy and Infectious Diseases (NIAID), one of the National Institutes of Health (NIH), aims to better understand the complex biochemical networks that regulate the interactions between infectious organisms and the human or animal cells they infect.
New cellular flaw found in some virulent breast cancers
Researchers at Dana-Farber Cancer Institute have identified a molecular interaction that triggers a particularly aggressive form of breast cancer, and suggest that attacking this target with selective drugs might improve treatment.
International PhD Program in Infection Biology starts at GBF
The EU "Miditrain" Program supports the elite education of young researchers. Twelve highly qualified young researchers from around the world will soon get the opportunity to advance their skills in Braunschweig. Beginning this fall, the German Research Centre for Biotechnology (GBF) will be coordinating the special European Union Marie Curie PhD program, MIDITRAIN. State-of-the art advanced training in molecular infection biology will be carried out together with the Hanover School of Medicine (MHH). Successful students will receive a PhD from the MHH. The EU supports MIDITRAIN with EUR 2 million by way of its Marie Curie Action program which is specially designed to increase training, mo
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