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Plants, plasmids and possibilities — Methods permit functional gene studies in plants
December 04, 2006Decaffeinated coffee plants, pest-resistant cotton, and Vitamin A-producing rice varieties have all been developed by introducing genes into plants. Scientists also create modified plants to identify and characterize the functions of specific genes. The current issue of Cold Spring Harbor Protocols-released online today (www.cshprotocols.org)-includes a set of techniques for the creation of transgenic plants.
One of the protocols, freely available at http://www.cshprotocols.org/cgi/content/full/2006/30/pdb.prot4668, describes the use of a bacterium, Agrobacterium, to create transgenic Arabidopsis plants. Arabidopsis is used in many studies due to its short reproductive cycle, ease of cultivation, and close relatedness to economically important species such as broccoli and cauliflower. Agrobacterium contains a small chromosome-called the Ti plasmid-into which scientists can insert a gene of interest. This 'transgene' is transferred to Arabidopsis through natural infection with Agrobacterium.
The highlighted article from CSH Protocols describes three techniques that encourage Agrobacterium to infect Arabidopsis plants: dipping an Arabidopsis flower directly into a solution containing Agrobacterium, mechanically forcing the Agrobacterium into the plant cells by applying vacuum, and simply spraying an Agrobacterium suspension onto the plants.
Cold Spring Harbor Laboratory
Cold Spring Harbor Laboratory
Agrobacterium News and Current Agrobacterium Events RSSScientists unravel the genetic coding of the pea
The pea is one of many important crop species that is unsuited to the Agrobacterium-based genetic modification techniques that are commonly used to work with crops.
Could 'hairy roots' become biofactories?
Rice University bioengineers have reported an advance in tapping the immense potential of "hairy roots" as natural factories to produce medicines, food flavorings and other commercial products.
Modified mushrooms may yield human drugs
Mushrooms might serve as biofactories for the production of various beneficial human drugs, according to plant pathologists who have inserted new genes into mushrooms.
Scientists expand microbe 'gene language'
An international group of scientists has expanded the universal language for the genes of both disease-causing and beneficial microbes and their hosts.
Costly plant tumors are found by Cornell microbiologist to be result of soil bacterium 'smelling' and entering wound
How does a wound in certain plants like roses and grapevines develop into a tumor? The answer appears to lie in a common soil bacterium that is able to "smell" the wound and speed up the infection process.
New approaches to the construction of genetically modified plants may avoid Syngenta and Monsanto patents
An article in the journal Nature published on 10th Feb (Gene transfer to plants by diverse species of bacteria) reports that a group of researchers in Canberra Australia have demonstrated that the ability to perform transfers of DNA from microbial cells to plant cells is not, as was previously assumed, restricted to Agrobacterium tumefaciens. When furnished with appropriately engineered DNA vectors a variety of Rhizobial species seem to be as potent a tool as Agrobacterium in the production of genetically modified plants. This offers the possibility of new approaches to the construction of genetically modified plants and may alter the commercial landscape and trajectories for a technology cu
GM Breakthrough Could Help Dutch Elm Disease Fight
A team of scientists from the University of Abertay Dundee has grown the world's first genetically modified elm trees. The breakthrough could lead to the reintroduction into their natural habitat of elm trees resistant to the Dutch elm disease (DED) fungus. Since 1970, more than 20 million elms in the UK have fallen victim to the environmentally devastating disease while, over the past 70 years, more than 70 per cent of US mature elms have been destroyed by DED. By transferring anti-fungal genes into the elm genome, Abertay researchers have produced genetically modified elms that could fight off the killer DED fungus, which rapidly spreads through infected trees. Their work is published in t
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| Gene Transfer to Plants (Springer Lab Manual) Using the clearly structured protocols given in this manual, it will be easy to apply the latest techniques in plant biotechnology in order to create new plant varieties or varieties with altered and optimized characteristics.Direct gene transfer into plant cells or protoplasts by the use of microinjection, electroporation or biolistic systems or mediated by Agrobacteria is described in detail... |
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