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Cold Spring Harbor Protocols highlights reliable methods for gene and protein analyses
April 05, 2007In their native form, the thousands of assorted proteins in our body are virtually indistinguishable. Scientists who want to examine the properties and functions of specific proteins, as well as the activities of individual genes, must rely on chemical tags to manipulate and visualize them. This month's release of Cold Spring Harbor Protocols highlights methods for creating and detecting specific proteins, as well as for characterizing the activities of specific genes during embryonic development. Two of the methods are freely available online (www.cshprotocols.org).
One of the freely available methods (http://www.cshprotocols.org/cgi/content/full/2007/8/pdb.prot4738) describes how to attach a small tag, called GST (glutathione-S-transferase), to a specific protein of interest. This is accomplished by growing bacteria that contain the DNA sequence for the GST tag adjacent to the gene for the protein of interest. When the bacteria grow, they express the "GST fusion protein," which consists of a GST tag attached to the desired protein. Using a chemical (glutathione) that binds specifically to GST, the GST fusion proteins can be easily sequestered and studied in more detail.
To test the activity of a certain gene during embryonic development, mice can be engineered to produce a "reporter" protein called â-galactosidase, which functions as a proxy for the protein normally produced by the gene of interest. The second freely available method (http://www.cshprotocols.org/cgi/content/full/2007/8/pdb.prot4725) describes a strategy for staining transgenic mouse embryos engineered to express the â-galactosidase protein. Once stained, the tissues with â-galactosidase produce a brilliant blue pigment, allowing researchers to visualize where and when a gene of interest is expressed during embryonic development.
Cold Spring Harbor Laboratory
Cold Spring Harbor Laboratory
Embryonic Development Current Events and Embryonic Development News RSS'Cross-talk' mechanism contributes to colorectal cancer
Researchers at the University of Wisconsin-Madison School of Medicine and Public Health have identified a molecular mechanism that allows two powerful signaling pathways to interact and begin a process leading to colorectal tumors.
Deciphering the regulatory code
Embryonic development is like a well-organised building project, with the embryo's DNA serving as the blueprint from which all construction details are derived.
The skeleton: Size matters
Vertebrates have in common a skeleton made of segments, the vertebrae. During development of the embryo, each segment is added in a time dependent manner, from the head-end to the tail-end: the first segments to be added become the vertebrae of the neck, later segments become the vertebrae with ribs and the last ones the vertebra located in the tail (in the case of a mouse, for example).
Liver cells grown from patients' skin cells
Scientists at The Medical College of Wisconsin in Milwaukee have successfully produced liver cells from patients' skin cells opening the possibility of treating a wide range of diseases that affect liver function.
Major improvements made in engineering heart repair patches from stem cells
University of Washington (UW) researchers have succeeded in engineering human tissue patches free of some problems that have stymied stem-cell repair for damaged hearts.
River flow and temperature limit trout numbers
Over a 23-year study, Javier Lobon-Cervia has found the mechanism that controls the number of salmonids found each year in Cantabrian rivers.
International conference on endothelin
One of the most intriguing developments in recent medical science is the discovery of the human chemical endothelin (ET).
Protein plays unexpected role protecting chromosome tips
A protein specialist that opens the genomic door for DNA repair and gene expression also turns out to be a multi-tasking workhorse that protects the tips of chromosomes and dabbles in a protein-destruction complex, a team lead by researchers at The University of Texas M. D. Anderson Cancer Center reports in the Aug. 13 edition of Molecular Cell.
Fox Chase Researchers Uncover One Force Behind the MYC Oncogene in Many Cancers
DLX5, a gene crucial for embryonic development, promotes cancer by activating the expression of the known oncogene, MYC, according to researchers from Fox Chase Cancer Center.
Sea lamprey jettison one-fifth of their genome
Researchers have discovered that the sea lamprey, which emerged from jawless fish first appearing 500 million years ago, dramatically remodels its genome. Shortly after a fertilized lamprey egg divides into several cells, the growing embryo discards millions of units of its DNA.
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Introduction to Embryonic Development (3rd Edition) by S. Oppenheimer (Author), G. Lefevre (Author) Ideal for those with little or no background in genetics and cellular or developmental biology, this exploration of embryology and the molecular aspects of development explains each concept from first principles. |
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Embryonic Development Model, 12 stages by American 3B Scientific Embryonic Development Model, 12 stages Embryonic Development Model, 12 stagesThis enlarged model represents the following stages of embryo development:• Ovule shortly after fertilization• Two-cell stage• Four-cell stage• Seven-cell stage• Morula stage• Blastocyst with trophoblast and embryoblast• Blastocyst with early formation of embryo process• Blastocyst with start of implantation• Embryo (approx. 12th day)• Embryo (approx. 20th day)• Embryo (approx. 28th day)• Embryo (approx. 2nd month)The first 8 models are enlarged approx. 4,000 times, the other 4 models are enlarged approx. 4-5 times. The first 8 stages can be removed from the baseboard for closer study. Delivered in storage carton.Measurements:12x59x41 cm Weight: 3,35 kg Product photo may not exactly match... |
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Em-bry-on-ics Mozart Magic Cube by Munchkin Ideal for children of all ages! Create original music from 5 different instruments, French horn, flute, piano, violin and harp or play music from eight Mozart compositions. Large colorful buttons light up to the tempo of the music. Soft, rounded corners make it easy for a baby to hold. Batteries included. |
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Biologix: Embryonic Development and Differentiation DVD by United Learning Describes the events that occur once fertilization of the ovum has taken place. |
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More Than Genes: What Science Can Tell Us About Toxic Chemicals, Development, and the Risk to Our Children by Dan Agin (Author) We are all shaped by our genetic inheritance and by the environment we live in. Indeed, the argument about which of these two forces, nature or nurture, predominates has been raging for decades. But what about our very first environment--the prenatal world where we exist for nine months between conception and birth and where we are more vulnerable than at any other point in our lives? In More Than Genes, Dan Agin marshals new scientific evidence to argue that the fetal environment can be just as crucial as genetic hard-wiring or even later environment in determining our intelligence and behavior. Stress during pregnancy, for example, puts women at far greater risk of bearing children prone to anxiety disorders. Nutritional deprivation during early fetal development may elevate... |
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The Embryonic Development of Drosophila melanogaster by Jose A. Campos-Ortega (Author), Volker Hartenstein (Author), Campos-Orteg (Author) The Drosophila embryo is one of the best understood developmental systems. Since the publication of the first edition over ten years ago, a lot of new information has accumulated. This has been taken into account in the extensively revised second edition of this standard reference book. This second edition is comprehensive, and describes and illustrates the embryonic development of Drosophila melanogaster. The anatomy of organogenesis is demonstrated in photographs and color diagrams of superb quality, showing sections of the various developmental stages. |
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Mozart Magic Flute by Embryonics Mozart Magic Flute - Em-bry-on-ics. Mozart Magic Flute helps your little one distinguish changes in pitch - a skill needed for the development of language skills including reading. |
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The Intrinsic Embryonic Development by G. Ten Cate (Author) | |
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Wnt Signaling in Embryonic Development, Volume 17 (Advances in Developmental) by Sergei Sokol (Editor) A superb compilation of reviews from leading experts in the field of Wnt signaling, volume 17 in the Advances in Developmental Biology series offers 7 chapters, each devoted to a separate area of research on Wnt signaling. Written by specialists in the field, these chapters cover different levels of signaling, including: ligands, receptors, cytoplasmic and nuclear components of the pathway. This book discusses a number of model systems including mice, Drosophila, Xenopus, zebrafish, C. elegans, and mammalian cells. Together, these chapters review the available knowledge and will fill gaps in our understanding of this interesting network of cell signaling processes. * Includes two parts devoted to the canonical (beta-catenin dependent) and... |
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Experimental Approaches to Mammalian Embryonic Development by Janet Rossant (Editor), Roger A. Pedersen (Editor) The editors present, in a single volume, a valuable reference source for students of mammalian embryology, genetics, and physiology, this book provides a thorough overview of the field of embryonic development--its current status along with possible directions in the future. |
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