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Microbiotic technology developed for microinjection of zebrafish embryos
September 12, 2007Funded by an NSERC Idea to Innovations grant and an Ontario Early Researcher Award, Prof. Yu Sun's group, the Advanced Micro and Nanosystems Laboratory (http://amnl.mie.utoronto.ca) at the University of Toronto (U of T) recently developed a microrobotic technology for automated microinjection of zebrafish embryos.
Based on computer vision and motion control, the automated microrobotic system is capable of immobilizing a large number of zebrafish embryos into a regular pattern within seconds and injecting 15 embryos (chorion unremoved) per minute with a success rate, survival rate, and phenotypic rate all close to 100%. The system and performance were reported in the journal PLoS ONE in an article entitled, "A Fully Automated Robotic System for Microinjection of Zebrafish Embryos."
Zebrafish is a model organism widely used in life sciences. High-speed injection of zebrafish embryos is important for screening genes in genetics and drug molecules in drug discovery. The automated microrobotic system proves itself as a reliable tool for determining gene functions and more generally, for facilitating large-scale molecule screening.
Public Library of Science
Public Library of Science
Zebrafish Current Events and Zebrafish News RSSNewly found enzymes may play early role in cancer
Researchers have discovered two enzymes that, when combined, could be involved in the earliest stages of cancer. Manipulating these enzymes genetically might lead to targeted therapies aimed at slowing or preventing the onset of tumors.
Mutations common to cancer and developmental disorder examined in a novel disease model
New research sheds light on a common link between tumor formation and Costello Syndrome, an inherited developmental disorder in which patients have cardiac defects, mild mental retardation, and face-shape abnormalities.
Can you hear me now? How the inner ear's sensors are made
A UCLA study shows for the first time how microscopic crystals form sound and gravity sensors inside the inner ear. Located at the ends of cilia - tiny cellular hairs in the ear that move and transmit signals - these crystals play an important role in detecting sound, maintaining balance and regulating movement.
International team discovers gene associated with epilepsy
A University of Iowa-led international research team has found a new gene associated with the brain disorder epilepsy.
Digital zebrafish embryo provides the first complete developmental blueprint of a vertebrate
Researchers at the European Molecular Biology Laboratory (EMBL) have generated a digital zebrafish embryo - the first complete developmental blueprint of a vertebrate. With a newly developed microscope scientists could for the first time track all cells for the first 24 hours in the life of a zebrafish.
Primordial fish had rudimentary fingers
Tetrapods, the first four-legged land animals, are regarded as the first organisms that had fingers and toes. Now researchers at Uppsala University can show that this is wrong. Using medical x-rays, they found rudiments of fingers in the fins in fossil Panderichthys, the "transitional animal," which indicates that rudimentary fingers developed considerably earlier than was previously thought.
Gladstone scientists identify single microRNA that controls blood vessel development
Scientists from the Gladstone Institute of Cardiovascular Disease (GICD) and UCSF have identified a key regulatory factor that controls development of the human vascular system, the extensive network of arteries, veins, and capillaries that allow blood to reach all tissues and organs.
New insight into most common forebrain malformation
St. Jude Children's Research Hospital scientists have identified one of the molecular mechanisms underlying the genetic brain malformation called holoprosencephaly (HPE). The findings not only yield insights into the most common developmental malformation of the anterior brain and face in newborns, but also help in understanding the intricate process by which the brain forms in the developing fetus.
Metabolic insight to illuminate causes of iron imbalance
New insight into key players in iron metabolism has yielded a novel tool for distinguishing among root causes of iron overload or deficiency in humans, the researchers report in the August issue of Cell Metabolism, a publication of Cell Press. While the body needs iron to produce hemoglobin, a substance in red blood cells that enables them to carry oxygen, too much iron can build up and eventually damage organs.
Pitt-led Research Provides Insight Into Development of Common Congenital Circulatory Defects
University of Pittsburgh-led researchers could provide new insight into how two common congenital circulatory problems-aortic arch deformity and arteriovenous malformations (AVMs)-develop in humans, as reported in the June 15 edition of Developmental Biology.
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