 |
|
Microbiotic technology developed for microinjection of zebrafish embryos
September 12, 2007Funded by an NSERC Idea to Innovations grant and an Ontario Early Researcher Award, Prof. Yu Sun's group, the Advanced Micro and Nanosystems Laboratory (http://amnl.mie.utoronto.ca) at the University of Toronto (U of T) recently developed a microrobotic technology for automated microinjection of zebrafish embryos.
Based on computer vision and motion control, the automated microrobotic system is capable of immobilizing a large number of zebrafish embryos into a regular pattern within seconds and injecting 15 embryos (chorion unremoved) per minute with a success rate, survival rate, and phenotypic rate all close to 100%. The system and performance were reported in the journal PLoS ONE in an article entitled, "A Fully Automated Robotic System for Microinjection of Zebrafish Embryos."
Zebrafish is a model organism widely used in life sciences. High-speed injection of zebrafish embryos is important for screening genes in genetics and drug molecules in drug discovery. The automated microrobotic system proves itself as a reliable tool for determining gene functions and more generally, for facilitating large-scale molecule screening.
Public Library of Science
Public Library of Science
Zebrafish Current Events and Zebrafish News RSSPenn Study Provides First Clear Idea of How Rare Bone Disease Progresses
An international team of scientists, led by researchers at the University of Pennsylvania School of Medicine, is taking the first step in developing a treatment for a rare genetic disorder called fibrodysplasia ossificans progressiva (FOP), in which the body's skeletal muscles and soft connective tissue turns to bone, immobilizing patients over a lifetime with a second skeleton.
Autism Consortium symposium draws record number of researchers, advocates, parents for autism update
The Autism Consortium, an innovative collaboration of researchers, clinicians, funders and families dedicated to catalyzing research and enhancing clinical care for autism spectrum disorders (ASDs), held its fourth annual symposium on October 28th, 2009, at Harvard Medical School in Boston.
Tiny injector to speed development of new, safer, cheaper drugs
It's no bigger than a stamp packet but it has the potential to allow rapid development of a new generation of drugs and genetic engineering organisms, and to better control in-vitro fertilization.
A master mechanism for regeneration?
Biologists long have marveled at the ability of some animals to re-grow lost body parts. Newts, for example, can lose a leg and grow a new one identical to the original. Zebrafish can re-grow fins.
Fish fend off invading germs with an initial response similar to the one found in people
Since the human response to infection is highly complex, research to understand how people fight infection is facilitated by studying how similar processes occur in simpler organisms.
With a flash of light, a neuron's function is revealed
There's a new way to explore biology's secrets. With a flash of light, scientists from the U.S. Department of Energy's Lawrence Berkeley National Laboratory and the University of California, Berkeley zeroed in on the type of neural cell that controls swimming in larval zebrafish.
Pitt team finds molecule that regulates heart size by using zebrafish screening model
Using zebrafish, researchers at the University of Pittsburgh have identified and described an enzyme inhibitor that allows them to increase the number of cardiac progenitor cells and therefore influence the size of the developing heart.
Embryology Study Offers Clues to Birth Defects
Gregg Duester, Ph.D., professor of developmental biology at Burnham Institute for Medical Research (Burnham), along with Xianling Zhao, Ph.D., and colleagues, have clarified the role that retinoic acid plays in limb development.
Hydrogen peroxide marshals immune system
When you were a kid your mom poured it on your scraped finger to stave off infection.
Zebrafish provide a model for cancerous melanoma in humans
In a new study published in Disease Models & Mechanisms, scientists use the zebrafish to gain insight into the influence of known cancer genes on the development and progression of melanoma, an aggressive form of human skin cancer with limited treatment options.
More Zebrafish Current Events and Zebrafish News Articles
 |
Zebrafish: Methods and Protocols (Methods in Molecular Biology) by Graham J. Lieschke (Editor), Andrew C. Oates (Editor), Koichi Kawakami (Editor) In the last 20 years, research activity using the zebrafish Danio rerio has increased dramatically, due in part to the ease of breeding and raising them, their genetic tractability, embryonic accessibility, and their imaging potential. In Zebrafish: Methods and Protocols, expert researchers provide a collection of experimental techniques for the genetic modification, manipulation, and in vivo live imaging of zebrafish. This volume collects sophisticated and practical techniques, including several not yet widely known, from laboratories around the world. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible... |
 |
Essential Zebrafish Methods: Cell and Developmental Biology (Reliable Lab Solutions) by Monte Westerfield (Editor), Leonard I. Zon (Editor), H. William Detrich III (Editor) Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues which make it the model organism for study of vertebrate development. This book will provide overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques. The methods and techniques in this volume were chosen by the editors of Methods in Cell Biology, whose goal was to provide fail-safe methods, tips, and "tricks of the trade" to experienced researchers and more junior members in the lab. ... |
 |
Zebrafish: A Practical Approach (The Practical Approach Series, 261) by Christiane Nusslein-Volhard (Editor), Ralf Dahm (Editor) The zebrafish has become one of the most important model organisms to study biologial processes within a living body. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. This book not only provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory. It also gives a broad overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques. |
 |
Essential Zebrafish Methods: Genetics and Genomics (Reliable Lab Solutions) by H. William Detrich III (Editor), Leonard I. Zon (Editor), Monte Westerfield (Editor) Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development. |
 |
ZEBRAFISH Street Sign ~ Fishing ~ Great Fish Sign for your Lodge, Gameroom, Basement, Garage, Barn by The Lizton Sign Shop ZEBRAFISH Street Sign A BRAND NEW SIGN!! Made of aluminum and high quality vinyl lettering and graphics this sign is 4 x 18 inches. Made to last for years outdoors the sign is nice enough to display indoors. Comes with two holes pre-punched for easy installation, corners are rounded. Great gift idea! WE A 100,000 SIGNS HERE ON AMAZON, PLEASE CHECK THEM ALL!!! |
 |
The Zebrafish: Genetics, Genomics and Informatics, Volume 77, Second Edition (Methods in Cell Biology) (Vol 77) by H. William Detrich III (Editor), Leonard I. Zon (Editor), Monte Westerfield (Editor) This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This second volume covers advances in forward and reverse genetic techniques, provides an update on the zebrafish genome and gene/mutant mapping technologies, examines the new systems for efficient transgenesis in the zebrafish, provides an in-depth view of informatics and the emerging field of comparative genomics, and considers the extensive infrastructure now available to the zebrafish community. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls *... |
 |
Zebrafish Zebrafish: Featuring Kate O'Brien (Primary Contributor) |
 |
ZEBRAFISH IRRAD DIET 16OZ - Adult Zebrafish Diet, Zeigler - Model 82031-061B - Bag by VWR Nutritionally complete diet for zebrafish reared in the laboratory. The diet is specially formulated to promote healthy fin and tissue development and has demonstrated increased fecundity in laboratory conditions. It utilizes highly digestible ingredients, which minimizes excreted waste thereby helping to maintain good water quality. The 0.6-0.84mm sinking crumble diet is formulated for low phosphorous effluent in water and is packaged in an irradiated, resealable 454g (16oz.) bag to ensure shelf stability. Manufactured under ISO 9001:2000 guidelines to ensure consistent, high quality. Ingredients include: fish meal, corn gluten meal, fish oil, blood flour, soy flour, wheat flour, wheat, liquid fish solubles, brewer's dried yeast, soy lecithin, calcium propionate, yeast culture,... |
 |
Fish Development And Genetics: The Zebrafish And Medaka Models (Molecular Aspects of Fish and Marine Biology) by Zhiyuan Gong (Editor), Vladimir Korzh (Editor) The zebrafish is the most important fish model in developmental and genetic analyses. This book contains 19 review articles covering a broad spectrum of topics, from development to genetic tools. The contents range from early development, the role of maternal factors and gastrulation, to tissue differentiation and organogenesis, such as development of the organizer, notochord, floor plate, nervous system, somites, muscle, skeleton and endoderm. The genetic tools cover morpholino knock-down, transgenics, fish cloning, transposons and genome evolution. The book also includes two chapters on genome mapping and embryonic stem cells in medaka, another important model fish. Summarizing the state-of-the-art studies of the zebrafish model and focusing on the molecular aspects of development, this... |
 |
The Zebrafish: Genetics and Genomics, Volume 60 (Methods in Cell Biology, Vol 60) by H. William Detrich III (Editor), Leonard I. Zon (Editor), Monte Westerfield (Editor), Leslie Wilson (Editor), Paul T. Matsudaira (Editor) This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Is relevant to clinicians interested in vertebrate models of human... |
| © 2009 BrightSurf.com |