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How is H pylori adhesion to gastric cells associated with MUC1 mucin VNTR size?
March 19, 2008The Gram negative bacterium H pylori is involved in the pathogenesis of several gastrointestinal diseases, ultimately leading to gastric carcinoma. Adhesion of the bacteria to the gastric mucosa is an essential step for colonization and infection.
It is therefore important to know factors that influence the binding of the bacteria to gastric cells. MUC1, a mucin with a highly variable repetitive region (VNTR), has been recently identified as an H pylori binding target. In this work we tested the hypothesis that MUC1 VNTR polymorphism affects H pylori adhesion to gastric cells and thus plays an important role in the colonization of gastric mucosa.
Enzyme linked immunosorbent assay (ELISA)-based adhesion assays were performed to measure the adhesion of different H pylori strains (HP26695 and HPTx30a) to gastric carcinoma cell lines (GP202 and MKN45) and GP202 clones expressing recombinant MUC1 with different VNTR lengths. The research article to be published on March 7, 2008 in the World Journal of Gastroenterology.
The results showed that MUC1 VNTR polymorphism influences the binding of H pylori to gastric cells, with the higher adhesion levels observed in clones with larger VNTR regions. Furthermore, higher adhesion was observed in co-cultures with the pathogenic strain (HP26695) when compared to the non-pathogenic strain (HPTx30a) and GP202 cell line when compared to the MKN45 cell line. This work contributes to the understanding of the interplay between host and bacterial factors in H pylori infection pathogenesis.
World Journal of Gastroenterology
The results showed that MUC1 VNTR polymorphism influences the binding of H pylori to gastric cells, with the higher adhesion levels observed in clones with larger VNTR regions. Furthermore, higher adhesion was observed in co-cultures with the pathogenic strain (HP26695) when compared to the non-pathogenic strain (HPTx30a) and GP202 cell line when compared to the MKN45 cell line. This work contributes to the understanding of the interplay between host and bacterial factors in H pylori infection pathogenesis.
World Journal of Gastroenterology
A potential route for human tumor gene therapy
The type 1 Na+/H+ exchanger (NHE1) is a transmembrane protein found in all eukaryotic cells. One of its functions is to evacuate excessive H+ in the cytoplasm by means of Na+-H+ exchange, resulting in stable intracellular pH value.
Gastric juice for diagnosis of H. pylori infection in patients on proton pump inhibitors
This study determined the efficiency of a gastric juice PCR test for the detection of H. pylori infection in patients receiving PPI therapy and compared it with histology and gastric biopsy PCR.
A new prognostic tool for gastric carcinomas? (and maybe other cancers)
A new way to identify gastric carcinoma patients with high probability of develop a more aggressive form of disease has just been described on the June issue of the journal Glycobiology.
Helicobacter pylori Acquisition Most Common In Young Children (P931)
A US study in this week's issue of THE LANCET highlights how most newly acquired infections of the intestinal bacterium Helicobacter pylori probably occur in children younger than 10 years of age. The authors of the study suggest that treatment and prevention strategies should therefore be targeted at young children. H pylori infection is associated with many gastro-intestinal disorders including gastritis, gastric ulcer, duodenal ulcer, gastric carcinoma, and primary gastric -cell lymphoma. H pylori is common worldwide, but the age at which it is acquired is unclear. Hoda Malaty and colleagues from Baylor College of Medicine, Houston, USA, studied 224 children (99 black, 125 white
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