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Cold Spring Harbor Protocols features methods to screen genomes and analyze evolution
April 02, 2008Identifying genes that are important in specific tissues or processes in the mouse used to be a monumental task. New technologies and strategies have simplified this search, making it effective for even the smallest laboratories. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc4_08.dtl) highlights a method for screening the mouse genome using ENU mutagenesis. The method, "Mouse Mutagenesis Using N-ethyl-N-nitrosourea (ENU)," was submitted by Monica Justice and colleagues from the Baylor College of Medicine http://www.bcm.edu/db/db_fac-justice.html). In her laboratory, Justice uses this "forward genetics" method to identify genes that may play a role in human disease. In particular, Justice's lab focuses on the process of hematopoiesis, the development of blood cells. Mutations in these genes can lead to leukemias or lymphomas. The method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/5/pdb.prot4985).
The second featured protocol for April is a guide for selecting the proper method for analyzing evolutionary relationships between genes. In "Choosing a Method for Phylogenetic Prediction," David Mount from the University of Arizona (http://bmcb.biology.arizona.edu/mount.html) provides a step by step process to determine which of the major methods one should use for predicting "phylogeny", the relatedness among gene sequences. The method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/5/pdb.ip49).
Cold Spring Harbor Laboratory
Mutagenesis Current Events and Mutagenesis News RSSTraDIS technique tackles typhoid
For the first time, researchers are able to look at the need for every gene in a bacterial cell in a single experiment. The new method will transform the study of gene activity and the search for weaknesses in bacterial armouries.
Genetic discovery could break wine industry bottleneck, accelerate grapevine breeding
One of the best known episodes in the 8000-year history of grapevine cultivation led to biological changes that have not been well understood - until now.
Fungal map of mutations key to increasing enzyme production for bioenergy use
In half a century, one fungus has gone from being the bane of the Army quartermasters' existence in the Pacific to industry staple and someday, as part of the U.S. Department of Energy's mission to promote national energy security through clean, renewable energy development, a biofuel producers' best friend.
Structural biology scores with protein snapshot
In a landmark technical achievement, investigators in the Vanderbilt Center for Structural Biology have used nuclear magnetic resonance (NMR) methods to determine the structure of the largest membrane-spanning protein to date.
Tool Helps Identify Gene Function in Soybeans, Could Lead to Better Crop Performance, say MU Researchers
In the race for bioengineered crops, sequencing the genome could be considered the first leg in a multi-leg relay.
Misreading of damaged DNA may spur tumor formation
The DNA in our cells is constantly under assault from oxygen, the sun's radiation and environmental stresses. Most of the time, our cells can repair the damage before it gets copied into a permanent mutation that could lead to cancer.
Study a step toward disease-resistant crops, sustainability
A five-year study that could help increase disease resistance, stress tolerance and plant yields is under way at Purdue University.
Forced evolution: Can we mutate viruses to death?
It sounds like a science fiction movie: A killer contagion threatens the Earth, but scientists save the day with a designer drug that forces the virus to mutate itself out of existence. The killer disease? Still a fiction. The drug? It could become a reality thanks to a new study by Rice University bioengineers.
The 21st century tomato
When tomatoes ripen in our gardens, we watch them turn gradually from hard, green globules to brightly colored, aromatic, and tasty fruits.
U of Saskatchewan distinguished researcher finds an SOS response to cancer-causing agents
University of Saskatchewan microbiologist Wei Xiao has found a way to trigger a protein combination called 9-1-1 that sends an SOS signal for cells to fight cancer-causing agents such as industrial toxins, ultraviolet radiation, and X-rays.
More Mutagenesis Current Events and Mutagenesis News Articles
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Mutagenesis by Helen Collins (Author) In the colony of Plain to recover strains of grasses long extinct on Earth, Dr. Mattie Manan has a falling out with its patriarchal leaders and must escape, along with some native women, into the plains of Anu. Reprint. PW. | |
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Targeted Mutagenesis in Mice [VHS] Starring: Janet Rossant | |
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DNA Repair And Mutagenesis by Errol C. Friedberg (Author), Graham C. Walker (Author), Wolfram Siede (Author), Richard D. Wood (Author), Roger A. Schultz (Author), Tom Ellenberger (Author) Featuring more than 10,000 references and a text lavishly complemented by over 700 illustrations, "DNA Repair and Mutagenesis, Second Edition" is a timely update to the original edition published in 1995. This work: features three new authors, including an expert in the field of structural biology, ensures a comprehensive review of the most current research in diverse subject areas; presents timely updates to the only comprehensive textbook in the field of DNA repair; offers contributions by recognized experts in the field; provides a strong historical context for comprehensive review of material; features a 12-page, full-color insert and over 700 illustrations, including protein structures; and, covers all aspects of biological responses to DNA damage. |
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Conditional Mutagenesis: An Approach to Disease Models (Handbook of Experimental Pharmacology) (Volume 178) by Robert Feil (Author), Robert Feil;Daniel Metzger (Editor) Leading experts provide timely and comprehensive information on methods for conditional mutagenesis in the mouse (part 1) and their application to model human physiology and pathophysiology (part 2). It illustrates how sophisticated genetic manipulations of the mouse genome are employed to model human diseases and to identify underlying molecular mechanisms. Finally the book considers the development of new drugs to treat them. |
![DNA Repair and Mutagenesis, 2nd Edition [A book review from: DNA Repair]](http://ecx.images-amazon.com/images/I/51FZ3K9Y7XL._SL160_.jpg) |
DNA Repair and Mutagenesis, 2nd Edition [A book review from: DNA Repair] by A.R. Lehmann (Author) This digital document is a journal article from DNA Repair, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: |
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Biotechnology Theory and Techniques Genetic Engineering Mutagenesis Separation Technology (Volume II) by Jack G. Chirikjian (Author) | |
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Progress Environ Mutagenesis: (Research Monographs in Cell and Tissue Physiology) by European Environmental Mutagen Society (Author), A. Ed. Kappas (Author) | |
![Induction of LacZ mutations in Muta(TM)Mouse can distinguish carcinogenic from non-carcinogenic analogues of diaminotoluenes and nitronaphthalenes [An ... Toxicology and Environmental Mutagenesis]](http://ecx.images-amazon.com/images/I/51VRJGWFK9L._SL160_.jpg) |
Induction of LacZ mutations in Muta(TM)Mouse can distinguish carcinogenic from non-carcinogenic analogues of diaminotoluenes and nitronaphthalenes [An ... Toxicology and Environmental Mutagenesis] by D. Kirkland (Author), C. Beevers (Author) This digital document is a journal article from Mut.Res.-Genetic Toxicology and Environmental Mutagenesis, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: 2,4-Diaminotoluene (2,4-DAT) is a liver carcinogen in rats and mice whereas 2,6-DAT is not. Both are genotoxic in vitro. Tests for mutations in transgenic mice, unscheduled DNA synthesis (UDS), DNA damage and enhancement of initiated foci in vivo have shown some discrimination between these two analogues, but only after oral administration. 1- and 2-nitronaphthalene (1- and 2-NNT) are also both genotoxic in vitro, although, unlike 2,4- and 2,6-DAT, they do not require... |
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Molecular mechanisms for maintenance of G-rich short tandem repeats capable of adopting G4 DNA structures [An article from: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis by H. Nakagama (Author), K. Higuchi (Author), E. Tanaka (Author), N. Tsuchiya (Author), N (Author) This digital document is a journal article from Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Abstract: Mammalian genomes contain several types of repetitive sequences. Some of these sequences are implicated in various specific cellular events, including meiotic recombination, chromosomal breaks and transcriptional regulation, and also in several human disorders. In this review, we document the formation of DNA secondary structures by the G-rich repetitive sequences that have been found in several minisatellites, telomeres and in various triplet repeats, and report... |
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Oxidative DNA damage induced by hair dye components ortho-phenylenediamines and the enhancement by superoxide dismutase [An article from: Mut.Res.-Genetic Toxicology and Environmental Mutagenesis] by M. Murata (Author), T. Nishimura (Author), F. Chen (Author), S. Kawanishi (Author) This digital document is a journal article from Mut.Res.-Genetic Toxicology and Environmental Mutagenesis, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser. Description: There is an association between occupational exposure to hair dyes and incidence of cancers. Permanent oxidant hair dyes are consisted of many chemical components including ortho-phenylenediamines. To clarify the mechanism of carcinogenesis by hair dyes, we examined DNA damage induced by mutagenic ortho-phenylenediamine (o-PD) and its derivatives, 4-chloro-ortho-phenylenediamine (Cl-PD) and 4-nitro-ortho-phenylenediamine (NO"2-PD), using ^3^2P-labeled DNA fragments obtained... |
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