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New cost-effective means to reconstruct virus populations
May 09, 2008Researchers from the United States and Switzerland have developed mathematical and statistical tools for reconstructing viral populations using pyrosequencing, a novel and effective technique for sequencing DNA. They describe their findings in an article published May 9th in the open-access journal PLoS Computational Biology.
The scientists knew that pyrosequencing reads are short and error-prone, and thus set out to improve upon this process. The new computational method they developed reduces the error rate and yields information faster and more efficiently. The method has been applied to four independent, diverse HIV-1 populations from drug-resistant patients and compared to 165 sequences obtained directly from clonal sequencing of the same samples.
"These new techniques produce results quite close to accepted techniques at a lower cost and potentially higher resolution," says Niko Beerenwinkel from ETH Zurich, one of the researchers.
Knowledge of the genetic structure of virus populations is critical for furthering biomedical research on disease progression, vaccine design, and drug resistance. The ability to better estimate the structure of virus populations holds great promise for new insights into viral evolution and disease control strategies.
http://www.ploscompbiol.org/doi/pcbi.1000074
Public Library of Science
Knowledge of the genetic structure of virus populations is critical for furthering biomedical research on disease progression, vaccine design, and drug resistance. The ability to better estimate the structure of virus populations holds great promise for new insights into viral evolution and disease control strategies.
http://www.ploscompbiol.org/doi/pcbi.1000074
Public Library of Science
Charting HIV's rapidly changing journey in the body
HIV is so deadly largely because it evolves so rapidly. With a single virus as the origin of an infection, most patients will quickly come to harbor thousands of different versions of HIV, all a little bit different and all competing with one another to most efficiently infect that person's cells.
Yale biologists 'trick' viruses into extinction
While human changes to the environment cause conservation biologists to worry about species extinction, Yale biologists are reversing the logic by trying to trap viruses in habitats that force their extinction, according to a report in Ecology Letters.
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