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Proteomics Study Yields Clues As To How Tuberculosis Might Be Thwarting The Immune System
November 06, 2008BERKELEY, CA - A link between the immune system and the self-cleaning system by which biological cells rid themselves of obsolete or toxic parts may one day yield new weapons in the fight against tuberculosis and other deadly infectious diseases. Scientists with the U.S. Department of Energy's (DOE) Lawrence Berkeley National Laboratory (Berkeley Lab) have discovered proteins residing in both systems that point to "cross-talk" between them.
In a collaboration between the research groups of Carolyn Bertozzi, director of Berkeley Lab's Molecular Foundry nanoscience center, and Jay Keasling, director of Berkeley Lab's Physical Biosciences Division, profiles were obtained for 546 different types of proteins in the membrane of a phagosome, an organelle of macrophages (a type of white blood cell) that essentially "eats" and destroys invading organisms (a process called phagocytosis). This represents the most comprehensive proteomic analysis of a phagosomal membrane to date.
"We were able to identify many new proteins that were not previously known to reside in the phagosome," said Wenqing Shui, a member of both the Bertozzi and Keasling research groups, and a proteomics specialist who was the lead author on a paper reporting these results in the Proceedings of the National Academy of Sciences (PNAS).
"One of the new proteins exclusively found in our study, LC3-II, is considered a marker of autophagy, the process that enables cells to clean up their own cytoplasm," Shui said. "Not only was LC3-II present in the phagosome, its level was increased upon the induction of autophagy in macrophages, and reduced when autophagy was suppressed. This indicates cross-talking between autophagy and phagocytosis that may play an important role in the response of the immune system."
The PNAS paper is entitled: "Membrane proteomics of phagosomes suggests a connection to autophagy." Co-authoring this paper in addition to Shui, Bertozzi and Keasling were Leslie Sheu, Jun Liuc, Brian Smart, Christopher Petzold, Tsung-yen Hsieh and Austin Pitcher. Bertozzi and Keasling are also professors at the University of California at Berkeley. In addition, Bertozzi is an investigator with the Howard Hughes Medical Institute, and Keasling is director of the Joint BioEnergy Institute.
When bacteria or other foreign particles invade the body, the first line of defense are the macrophages, which engulf and contain the invaders within the membrane-bound shells of their phagosomes. Once safely contained, the invaders can be killed with digestive enzymes from another cell organelle, called a lysosome, which fuses with the phagosome. Macrophages, like other kinds of cells, also use lysosomal enzymes for internal housekeeping. However, until now there has been no direct biochemical evidence of a link between phagocytosis and autophagy.
Working with latex bead-containing phagosomes isolated in cell lines from mice, Shui and her colleagues performed a detailed analysis of the protein contents of the phagosomal membrane. Unlike earlier proteomic studies, which profiled the entire organelle and focused on abundant water-soluble protein species, the study by the Bertozzi-Keasling groups was membrane-specific and included hydrophobic protein species that are present in the membrane in relatively low amounts.
"We were able to demonstrate the endogenous level of LC3-II in macrophage phagosomes through the combination of sensitive proteomic techniques and biochemical assays," said Shui. "This is an excellent show-case of how a non-biased high-throughput proteomic study can shed new light on the diverse functions and pathways an organelle may engage in."
The LC3-II protein is a critical component of the autophagy machinery and the discovery that the level of its presence in phagosomes is modulated by autophagic activity (along with several other newly identified phagosome proteins not previously associated with autophagy), points to autophagy playing a heretofore unknown role in immune response, particularly against intracellular pathogens such as mycobacterium tuberculosis.
As Shui explained, "After mycobacterium tuberculosis are phagocytosed into the macrophage cell, they are able to subvert various host defense mechanisms, including the killing of bacilli in the phagosome, and survive well inside the cell. Given that induced autophagy activity appears to enhance mycobacterial killing in the phagosome (from a study by the University of New Mexico's Vojo Deretic and his research group), we speculate that mycobacteria may produce specific factors to counteract the bactericidal effect of autophagy activation."
The Bertozzi and Keasling research groups are now examining whether certain mycobacterial products can modulate macrophage autophagy activity. They are also looking for proteins that could specifically mediate the autophagy as well as the phagosome maturation process.
Said Shui, "We might be able to open new avenues for pharmacologic intervention of tuberculosis as well as other infectious diseases."
This research was supported by grants from the National Institutes of Health and the U.S. Department of Energy's Genomics:GTL program.
Berkeley Lab is a U.S. Department of Energy national laboratory located in Berkeley, California. It conducts unclassified scientific research and is managed by the University of California. Visit our Website at www.lbl.gov/
Lawrence Berkeley National Laboratory
"One of the new proteins exclusively found in our study, LC3-II, is considered a marker of autophagy, the process that enables cells to clean up their own cytoplasm," Shui said. "Not only was LC3-II present in the phagosome, its level was increased upon the induction of autophagy in macrophages, and reduced when autophagy was suppressed. This indicates cross-talking between autophagy and phagocytosis that may play an important role in the response of the immune system."
The PNAS paper is entitled: "Membrane proteomics of phagosomes suggests a connection to autophagy." Co-authoring this paper in addition to Shui, Bertozzi and Keasling were Leslie Sheu, Jun Liuc, Brian Smart, Christopher Petzold, Tsung-yen Hsieh and Austin Pitcher. Bertozzi and Keasling are also professors at the University of California at Berkeley. In addition, Bertozzi is an investigator with the Howard Hughes Medical Institute, and Keasling is director of the Joint BioEnergy Institute.
When bacteria or other foreign particles invade the body, the first line of defense are the macrophages, which engulf and contain the invaders within the membrane-bound shells of their phagosomes. Once safely contained, the invaders can be killed with digestive enzymes from another cell organelle, called a lysosome, which fuses with the phagosome. Macrophages, like other kinds of cells, also use lysosomal enzymes for internal housekeeping. However, until now there has been no direct biochemical evidence of a link between phagocytosis and autophagy.
Working with latex bead-containing phagosomes isolated in cell lines from mice, Shui and her colleagues performed a detailed analysis of the protein contents of the phagosomal membrane. Unlike earlier proteomic studies, which profiled the entire organelle and focused on abundant water-soluble protein species, the study by the Bertozzi-Keasling groups was membrane-specific and included hydrophobic protein species that are present in the membrane in relatively low amounts.
"We were able to demonstrate the endogenous level of LC3-II in macrophage phagosomes through the combination of sensitive proteomic techniques and biochemical assays," said Shui. "This is an excellent show-case of how a non-biased high-throughput proteomic study can shed new light on the diverse functions and pathways an organelle may engage in."
The LC3-II protein is a critical component of the autophagy machinery and the discovery that the level of its presence in phagosomes is modulated by autophagic activity (along with several other newly identified phagosome proteins not previously associated with autophagy), points to autophagy playing a heretofore unknown role in immune response, particularly against intracellular pathogens such as mycobacterium tuberculosis.
As Shui explained, "After mycobacterium tuberculosis are phagocytosed into the macrophage cell, they are able to subvert various host defense mechanisms, including the killing of bacilli in the phagosome, and survive well inside the cell. Given that induced autophagy activity appears to enhance mycobacterial killing in the phagosome (from a study by the University of New Mexico's Vojo Deretic and his research group), we speculate that mycobacteria may produce specific factors to counteract the bactericidal effect of autophagy activation."
The Bertozzi and Keasling research groups are now examining whether certain mycobacterial products can modulate macrophage autophagy activity. They are also looking for proteins that could specifically mediate the autophagy as well as the phagosome maturation process.
Said Shui, "We might be able to open new avenues for pharmacologic intervention of tuberculosis as well as other infectious diseases."
This research was supported by grants from the National Institutes of Health and the U.S. Department of Energy's Genomics:GTL program.
Berkeley Lab is a U.S. Department of Energy national laboratory located in Berkeley, California. It conducts unclassified scientific research and is managed by the University of California. Visit our Website at www.lbl.gov/
Lawrence Berkeley National Laboratory
Autophagy Current Events and Autophagy News RSSCancer metabolism discovery uncovers new role of IDH1 gene mutation in brain cancer
Agios Pharmaceuticals today announced that its scientists have established, for the first time, that the mutated IDH1 gene has a novel enzyme activity consistent with a cancer-causing gene, or oncogene.
Inhibitor of heat shock protein is a potential anticancer drug, Penn study finds
Like yoga for office drones, cells do have coping strategies for stress. Heat, lack of nutrients, oxygen radicals - all can wreak havoc on the delicate internal components of a cell, potentially damaging it beyond repair.
HIV uses autophagy for its own means
Not satisfied with simply thwarting its host's defensive maneuvers, HIV actually twists one to its advantage, based on new findings from Kyei et al. in the July 27, 2009 issue of the Journal of Cell Biology (www.jcb.org). Vojo Deretic and colleagues suggest that autophagy-a stress response process-helps HIV to proliferate and that conversely, blocking autophagy lessens HIV production.
Waste disposal protein is mechanism behind cancer tumor suppression
"Taking out the trash" takes on a whole new meaning, as investigators at The Cancer Institute of New Jersey (CINJ) and Rutgers, The State University of New Jersey, have discovered that a waste disposal protein is the key to cancer tumor suppression in a process known as autophagy.
Scientists advance safety of nanotechnology
Scientists have identified for the first time a mechanism by which nanoparticles cause lung damage and have demonstrated that it can be combated by blocking the process involved, taking a step toward addressing the growing concerns over the safety of nanotechnology.
Muscle atrophy through thick but not thin
During desperate times, such as fasting, or muscle wasting that afflicts cancer or AIDS patients, the body cannibalizes itself, atrophying and breaking down skeletal muscle proteins to liberate amino acids.
Enhancing the effects of the drug used to treat chronic myeloid leukemia
Individuals with chronic myeloid leukemia (CML) are first treated with a drug known as imatinib mesylate. Although very effective, as the disease progresses it often becomes resistant to the drug.
Therapeutic effect of imatinib improved with addition of chloroquine
The therapeutic effects of the blockbuster leukemia drug imatinib may be enhanced when given along with a drug that inhibits a cell process called autophagy, researchers from the Kimmel Cancer Center at Jefferson reported in the Journal of Clinical Investigation.
TB breakthrough could lead to stronger vaccine
A breakthrough strategy to improve the effectiveness of the only tuberculosis vaccine approved for humans provided superior protection against the deadly disease in a pre-clinical test, report scientists at The University of Texas Health Science Center at Houston in Nature Medicine's Advance Online Publication March 1.
Self-digestion as a means of survival
In times of starvation, cells tighten their belts: they start to digest their own proteins and cellular organs. The process - known as autophagy - takes place in special organelles called autophagosomes.
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