RSS Feeds

Earth Science

Space Science

Life Science

Fields of Scientific Study

Nanotechnology

Cancer

Definitions of The Fields of Scientific Study

Cold Spring Harbor Protocols features tissue culture methods for immune cells and neurons

December 03, 2008

The complexity of vital organ systems makes them difficult to study in living organisms. Tissue culture methods for specific cell types allow researchers to break these systems down into component parts that can be readily manipulated and observed. This month's issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc12_08.dtl) features two articles detailing experimental culture methods for cells from the immune system and the nervous system.

"Bone Marrow-Derived Macrophages (BMM): Isolation and Applications," from Bo Porse and colleagues at the University of Copenhagen (http://www.bric.ku.dk/research/porse_group/), describes the derivation and growth of bone marrow-derived macrophages. Macrophages are specialized cells that carry out numerous tasks in the immune system such as phagocytosis, antigen presentation, cytokine production and migration. Cultured cells can be used to study these functions and to perform assays for gene expression, gene function and interaction with microbes. The protocol is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/13/pdb.prot5080).

Primary cultures of granule neurons from the cerebellum provide an excellent model system for molecular and cell biological studies of neuronal development and function. Many fundamental insights into the processes of neuronal apoptosis, migration, and differentiation in the mammalian central nervous system have come from investigating granule neurons in vitro. Azad Bonni's laboratory at Harvard University (http://pathology.hms.harvard.edu/labs/bonni/index.html) provides "Cultures of Cerebellar Granule Neurons," a protocol for isolating and culturing these cells. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://www.cshprotocols.org/cgi/content/full/2008/13/pdb.prot5107).

Cold Spring Harbor Laboratory

Related Neurons Current Events and Neurons News Articles

Neurons Current Events and Neurons News RSS

Scripps research scientists find new link between insulin and core body temperature
A team led by scientists at The Scripps Research Institute have discovered a direct link between insulin-a hormone long associated with metabolism and metabolic disorders such as diabetes-and core body temperature.

New Down syndrome treatment suggested by Stanford/Packard study in mice
At birth, children with Down syndrome aren't developmentally delayed. But as they age, these kids fall behind. Memory deficits inherent in Down syndrome hinder learning, making it hard for the brain to collect experiences needed for normal cognitive development.

Cognitive dysfunction reversed in mouse model of Down syndrome
A study by neuroscientist William C. Mobley, MD, PhD, chair of the Department of Neurosciences at the University of California, San Diego School of Medicine, and colleagues at Stanford University Medical School has demonstrated a possible new approach to slowing the inevitable progression of cognitive decline found in Down's syndrome.

Pushing the brain to find new pathways
Until recently, scientists believed that, following a stroke, a patient had about six months to regain any lost function. After that, patients would be forced to compensate for the lost function by focusing on their remaining abilities.

Scientists decipher the formation of lasting memories
Researchers at Karolinska Institutet have discovered a mechanism that controls the brain's ability to create lasting memories. In experiments on genetically manipulated mice, they were able to switch on and off the animals' ability to form lasting memories by adding a substance to their drinking water.

Developmental delay could stem from nicotinic receptor deletion
The loss of a gene through deletion of genetic material on chromosome 15 is associated with significant abnormalities in learning and behavior, said a consortium of researchers led by Baylor College of Medicine (www.bcm.edu) in a report that appears online today in the journal Nature Genetics.

New TMS clinic offers noninvasive treatment for major depression
Rush University Medical Center has opened the Transcranial Magnetic Stimulation (TMS) Clinic to offer patients suffering from major depression a safe, effective, non-drug treatment.

Researchers explore new ways to prevent spinal cord damage using a vitamin B3 precursor
Substances naturally produced by the human body may one day help prevent paralysis following a spinal cord injury, according to researchers at Weill Cornell Medical College. A recent $2.5 million grant from the New York State Spinal Cord Injury Research Board will fund their research investigating this possibility.

Estrogen therapy likely must be given soon after menopause to provide stroke protection
For estrogen replacement to provide stroke protection, it likely must be given soon after levels drop because of menopause or surgical removal of the ovaries, scientists report in the Journal of Neuroscience.

Researchers identify drug candidate for treating spinal muscular atrophy
A chemical cousin of the common antibiotic tetracycline might be useful in treating spinal muscular atrophy (SMA), a currently incurable disease that is the leading genetic cause of death in infants.

More Neurons Current Events and Neurons News Articles

	BRAIN CELL (NEURON) GIANT MICROBES PLUSH [Toy] [Toy] by Giant Microbes The more brain cells you have, the smarter you are.
	The Neuron: Cell and Molecular Biology by Irwin B. Levitan Ph.D. (Author), Leonard K. Kaczmarek Ph.D. (Author) The third edition of The Neuron provides a comprehensive first course in the cell and molecular biology of nerve cells. The first part of the book covers the properties of the many newly discovered ion channels that have emerged through mapping of the genome. These channels shape the way a single neuron generates varied patterns of electrical activity. Next are covered the molecular mechanisms that convert electrical activity into the secretion of neurotransmitter hormones at synaptic junctions between neurons. The second part of the book covers the biochemical pathways that are linked to the action of neurotransmitters and that can alter the cellular properties of neurons or sensory cells that transduce information from the outside world into the electrical code used by neurons. The...
	Brain Cell (Neuron) Giganticmicrobes By Giant Microbes [Toy] [Toy] by Giant Microbes The new LARGER size microbe plush measure 15 inches to 20 inches.
	Knowing Neurons Clearance Sale Art Poster Print, 18x24 by AllPosters.com AllPosters.com is the world's #1 seller of posters, prints, photographs, specialty products and framed art. We're dedicated to bringing our customers the best selection of high quality wall décor that is perfect for their home or office. Browse our catalog of over 300,000 items that include entertainment and specialty posters, decorative prints, and art reproductions. Whether you're looking for your favorite movie or music poster, a framed Monet reproduction, or a print of the Eiffel Tower you will find it at AllPosters.com. Visit our Amazon store today at www.amazon.com/allposters to find Special Offers and search by subject category or artist. AllPosters.com provides unmatched service with a 100% satisfaction guarantee. We ship internationally to over 80 countries. Decorate your...
	From Neurons to Neighborhoods : The Science of Early Childhood Development by Committee on Integrating the Science of Early Childhood Development (Author), Youth, and Families Board on Children (Author), Committee on Integrating the Science of Early Childhood Development (Author), National Research Council (Author), Jack P. Shonkoff (Editor), Deborah A. Phillips (Editor) Authoritative yet accessible, Neurons to Neighborhoods presents the newest evidence about early brain development and how children learn to speak, think, get along with others, and regulate their behavior. It examines the effect of the surrounding context-family, child care, community-within which the child grows. Are the early years a time of vulnerability or resilience? To what extent are our future prospects constrained by how well we navigate them? How can we get all children off to a good start in life? When should we worry? The committee provides a framework for approaching such vital questions and explores how scientists know what they know about childhood development. This timely release will be important to all those who care about children and their families:...
	Knowing Neurons Education Art Poster Print, 18x24 by Art.com Art.com is the world's largest retailer of art prints, posters, photographs, and framed artwork. With our huge selection of over 400,000 prints, you'll easily find the perfect piece for your home, office, or classroom. Our art is printed on quality paper. When you order framed artwork, the piece is built by our team of in-house professionals. Visit our Amazon store today at www.amazon.com/artdotcom to find Special Offers and search for products based on 'Artist Name' and 'Subject Categories' such as Movie, Music, Vintage, TV, Children, Travel, Kitchen, Museum Art, Animals, Floral, Motivational, and Sports. Art.com is dedicated to providing you with high quality products and service by offering you 100% satisfaction guaranteed. We ship internationally to over 80 countries. Decorate your...
	DANCE by The Neurons [CD on Demand] by THE NEURONS
	I of the Vortex: From Neurons to Self by Rodolfo R. Llinas (Author) In I of the Vortex, Rodolfo Llinás, a founding father of modern brain science, presents an original view of the evolution and nature of mind. According to Llinás, the "mindness state" evolved to allow predictive interactions between mobile creatures and their environment. He illustrates the early evolution of mind through a primitive animal called the "sea squirt." The mobile larval form has a brainlike ganglion that receives sensory information about the surrounding environment. As an adult, the sea squirt attaches itself to a stationary object and then digests most of its own brain. This suggests that the nervous system evolved to allow active movement in animals. To move through the environment safely, a creature must anticipate the outcome of each movement on the basis of incoming...
	Scientific Brain Training--Brain Fitness CD-ROM (PC) by Happy Neuron This product is a revolutionary new way to improve your thinking ability quickly and easily! 20 minutes per day is all that is needed to greatly improve your cognitive skills! Brain Fitness will give your mind a workout that tests your recent memory, language skills, concentration, and ability to decipher, classify and order concepts. Brain Fitness is vital for success in daily life for everyone, whether you are a student, professional, baby boomer or senior. This CD-ROM contains fun and innovative exercises and tools that will provide your brain with a full mental workout. Your brain requires complex stimulation to build brainpower and this program provides just that! In just 20 minutes per day, Brain Fitness will help increase thinking ability, prevent brain aging, and hone all five...
	T-Shirt Mens Navy Blue " Blogging is good for neuron development " Hobbies Large by TopExpressions