A sticky business -- how cancer cells become more 'gloopy' as they dieMarch 16, 2009The viscosity, or 'gloopiness', of different parts of cancer cells increases dramatically when they are blasted with light-activated cancer drugs, according to new images that provide fundamental insights into how cancer cells die, published in Nature Chemistry today (15 March). The images reveal the physical changes that occur inside cancer cells whilst they are dying as a result of Photodynamic Therapy (PDT). This cancer treatment uses light to activate a drug that creates a short-lived toxic type of oxygen, called singlet oxygen, which kills cancerous cells. The research team behind the study says that revealing what happens to viscosity within a dying cancer cell is important because it helps give a better understanding of how cells function and which factors are important for controlling reactions inside cells. Ultimately this could help scientists design more efficient drugs for Photodynamic Therapy and other treatments. The research is also of wider significance because these are the first ever real-time maps showing viscosity changing over a period of time inside a cell during a biologically important process like cell death. Previous studies have shown that the viscosity of human cells and organs also changes in patients with diseases including diabetes and atherosclerosis, says lead author Dr Marina Kuimova from Imperial College London's Department of Chemistry. She explains: "We're still not quite sure exactly what the relationship is between increased stickiness inside cells and disease, but we expect that the two are related." "Knowing more about these changes, and being able to map them when they occur in all kinds of different scenarios, from dying cancer cells, to diseased blood cells, could help us to better understand how some diseases and their treatments affect cell and organ function." Dr Kuimova and her colleagues were able to track viscosity as it changed inside live cancer cells thanks to a newly-developed Photodynamic Therapy drug, with unusual fluorescent properties. The drug, which is made of a molecule with a spinning component like a rotor, emits different wavelengths of light depending on the viscosity of its surroundings. The changing wavelengths of light emitted during experiments, and captured over a period of 10 minutes, showed that once the PDT drug was activated, the level of viscosity inside the cell increased dramatically. The researchers suggest that this increasing 'gloopiness' is caused by the toxic oxygen molecules released into the cell. They think that increased levels of viscosity might even contribute directly to the cancer cell's further deterioration by slowing down vital communication and transport processes inside the cell. Dr Stanley Botchway from the Science and Technology Facilities Council which worked in collaboration with Imperial College London on this research, said: "The huge viscosity we measured was surprising and it certainly gives a new insight into the change in cellular environment during cell death." However, the researchers noted that as viscosity in the cancerous cell increases, the toxic oxygen molecule's mission to kill the cell is slowed down too. Dr Kuimova explains: "It looks like whilst the increasing viscosity contributes to the cell's demise, these new 'sticky' cell conditions can slow the drug down too, so it's not as straightforward a relationship as it might first appear. "More work is needed to better understand the complex interplay between viscosity and cell death. We hope to use our imaging technique to track changes in viscosity in other kinds of cells as they occur in real-time, to unlock some of the secrets of what goes on inside cells when they're functioning, malfunctioning or dying." Imperial College London |
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| Related Viscosity Current Events and Viscosity News Articles New nanochemistry technique encases single molecules in microdroplets Inventing a useful new tool for creating chemical reactions between single molecules, scientists at the National Institute of Standards and Technology (NIST) have employed microfluidics-the manipulation of fluids at the microscopic scale-to make microdroplets that contain single molecules of interest. Multi-laboratory study sizes up nanoparticle sizing As a result of a major inter-laboratory study, the standards body ASTM International has been able to update its guidelines for a commonly used technique for measuring the size of nanoparticles in solutions. Researchers link jellyfish, other small sea creatures to large-scale ocean mixing The ocean's smallest swimming animals, such as jellyfish, can have a huge impact on large-scale ocean mixing, researchers have discovered. Green industrial lubricant developed A team of researchers from the University of Huelva has developed an environmentally-friendly lubricating grease based on ricin oil and cellulose derivatives, according to the journal Green Chemistry. Stirred, not shaken: Bio-inspired cilia mix medical reagents at small scales The equipment used for biomedical research is shrinking, but the physical properties of the fluids under investigation are not changing. Ancient volcanic eruptions caused global mass extinction A previously unknown giant volcanic eruption that led to global mass extinction 260million years ago has been uncovered by scientists at the University of Leeds. Back to basics: Scientists discover a fundamental mechanism for cell organization Scientists have discovered that cells use a very simple phase transition -- similar to water vapor condensing into dew -- to assemble and localize subcellular structures that are involved in formation of the embryo. New contraceptive device is designed to prevent sexual transmission of HIV Researchers from Weill Cornell Medical College have published results showing that a new contraceptive device may also effectively block the transmission of the HIV virus. Viscosity-enhancing nanomaterials may double service life of concrete Engineers at the National Institute of Standards and Technology (NIST) are patenting a method that is expected to double the service life of concrete. Researchers cooking up new gelled rocket fuels Engineers and food scientists are teaming up to develop a new type of gelled fuel the consistency of orange marmalade designed to improve the safety, performance and range of rockets for space and military applications. More Viscosity Current Events and Viscosity News Articles |
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