Is transforming growth factor-beta involved in intestinal wound healing?

March 31, 2009

Migration of colonic lamina propria fibroblasts (CLPF) plays an important role during the progression of fibrosis and fistulae in Crohn's disease. Transforming growth factor- beta (TGF- beta) is involved in the regulation of cell migration, cell differentiation, extracellular matrix deposition, and immune responses. Since the regulation of migration and differentiation of intestinal fibroblasts is an important mechanism during intestinal wound healing and fibrosis, it is important to investigate the effect of TGF- beta 1 on these processes and on fibronectin (FN) and FN isoform production

A research team led by Dr. Julia Brenmoehl from University of Jena addressed this issue and their study will be published on March 28, 2009 in the World Journal of Gastroenterology.

In their study, primary CLPF cultures were incubated with TGF-beta 1 and analyzed for production of alpha-smooth muscle actin (alpha-SMA), FN and FN isoforms. Migration assays were performed in a modified 48-well Boyden chamber. Levels of total and phosphorylated focal adhesion kinase (FAK) in CLPF were analyzed after induction of migration.

They found that incubation of CLPF with TGF-beta 1 for 2 days did not change alpha-SMA levels, while TGF-beta 1 treatment for 6 day significantly increased alpha-SMA production. Short term incubation (6 hours) with TGF-beta 1 enhanced CLPF migration, while long term treatment (6 day) of CLPF with TGF-beta 1 reduced migration to 15%-37% compared to untreated cells. FN and FN isoform mRNA expression were increased after short term incubation with TGF-beta 1 (2 days) in contrast to long term incubation with TGF-beta 1 for 6 days. After induction of migration, TGF-beta 1-preincubated CLPF showed higher amounts of FN and its isoforms and lower levels of total and phosphorylated FAK than untreated cells.

Their results indicated long term incubation of CLPF with TGF-beta 1 induced differentiation into myofibroblasts with enhanced alpha-SMA, reduced migratory potential and FAK phosphorylation, and increased FN production. In contrast, short term contact (6 hours) of fibroblasts with TGF-beta 1 induced a dose-dependent increase of cell migration and FAK phosphorylation without induction of alpha-SMA production. This study adds to the understanding of the role of TGF- beta in intestinal wound healing and stricture formation.

World Journal of Gastroenterology

---

---

	Intestinal Regeneration (Medical Intelligence Unit) by Shailendra K. Saxena (Author), Jon S. Thompson (Author), John G. Sharp (Author) Addresses current knowledge regarding intestinal regeneration and regulation. Specific chapters focus on the regulation of this complex process at both the tissue and cellular levels. Other chapters focus on the clinical potential and surgical implications.
	The New Healing Herbs: The Essential Guide to More Than 125 of Nature's Most Potent Herbal Remedies by Michael Castleman (Author) Straight from nature’s medicine cabinet, the latest herbal discoveries that cure hundreds of health concerns without the dangerous side effects or high cost of prescription drugsThe New Healing Herbs uses the latest studies to offer guidelines on dosages, drug interactions, and results for herbal healing. Included are herbal prescriptions to treat ailments ranging from the common cold, allergies, and back pain to more serious conditions such as diabetes and cancer. Featuring hundreds of cures and therapies proven to work, this book shows you which herbal remedy to take for each condition, how it’s taken, what interactions to watch for, and where to buy the featured herbs. The easy-to-use Cure Finder organizes herbs by health condition, healing actions, and alternative uses, guiding...
	Natural Stomach Care: Treating and Preventing Digestive Disorders with the Best of Eastern and Western Healing Therapies by Anil Minocha (Author) It's been said that health begins in the stomach. When digestion is not working properly, the whole body suffers. Skin rash, bad breath, headache, fatigue, overweight, and premature aging can all result from poor digestion-not to mention gastric disorders like heartburn, indigestion, and irritable bowel syndrome. But nearly half of digestive disorders can be averted with proper eating, exercise, stress management, and related lifestyle modifications. In Natural Stomach Care, gastroenterologist Anil Minocha, M.D., combines the wisdom of traditional Eastern healing with the advancements of conventional Western medicine to offer a wide range of easy-to-use, affordable, and effective at-home natural therapies that can strengthen digestion, keep the colon clean and the bowels moving, and...
	Gastrointestinal Disorders and Nutrition by Tonia Reinhard (Author) Gastrointestinal diseases profoundly affect a person's well being. When the GI system fails, nutritional status suffers and the person is unable to support a healthy immune system, making him or her more vulnerable to illness and infection. This comprehensive guide takes an in depth look at the role the gastrointestinal tract plays in maintaining life and health. The author provides a helpful overview of how the GI tract works and the types of problems that can arise. She also explores the new research into the causes and treatment of the diseases, with special emphasis on nutrition regimens.
	Nursing Care Plans: Diagnoses, Interventions, and Outcomes, 7e by Meg Gulanick (Author), Judith L. Myers (Author) The most comprehensive nursing care planning book available, Nursing Care Plans, 7th Edition features more than 200 care plans covering the most common medical-surgical nursing diagnoses and clinical problems. As in past editions, authors Meg Gulanick and Judith Myers meticulously updated content to ensure it reflects the most current clinical practice and professional standards in nursing, while still retaining the easy-to-use, reader-friendly format that make this book so unique. Functioning as two books in one, it provides you with both a collection of 68 nursing diagnosis care plans to use as starting points for creating individualized care plans and a library of 143 disease-specific care plans for medical-surgical conditions most frequently encountered in nursing practice.Includes...
	The Hempnut Cookbook: Tasty, Omega-Rich Meals from Hempseed by Richard Rose (Author), Brigitte Mars (Author), Christina Pirello (Author) Hempseed is the amazing 5,000-year-old super food from China. The "original nutraceutical," it's high in essential fatty acids (EFAs), complete protein, and much more. It has a long history of use in the treatment of degenerative diseases, and hempseed oil, the single richest source of omega-3 and omega-6 EFAs. This is the most comprehensive look at this amazing ancient food, its history, nutritional benefits, and uses. Over 200 recipes showcase the versatility of hempseed in everything from beverages and sauces to main dishes and desserts.
	Antimicrobial Therapy in Abdominal Surgery: Precepts and Practices by Haragopal Thadepalli (Author), Askis K. Mandal (Author) Over the past 50 years, important developments have taken place in surgical and antimicrobial aspects of the management of intestinal surgery and intra-abdominal sepsis. Many new pathogens like Clostridium difficile, Helibacter and Ruminococcus have been described, and many new beta-lactam drugs and quinolones have been introduced. Immunological aspects such as T cell factors and interleukins have been shown to play an important role in the pathogenesis of intra-abdominal sepsis. Important new pieces of surgical hardware, sutures, and surgical glues to salvage solid organs were added to the surgical armanentorium. This new book looks at all of these important developments and more. Over 900 articles were reviewed to synthesize the core information for this book. The editors' years of...
	The Most Effective Natural Cures on Earth: The Surprising, Unbiased Truth about What Treatments Work and Why by Jonny Bowden Ph.D. C.N.S. (Author) A comprehensive look at natural treatments and healing methods that work.Jonny Bowden takes his practical, knowledgeable, and open-minded approach—the same approach that made The 150 Healthiest Foods on Earth so successful—and focuses it on natural cures, revealing the best of alternative medicine for a mainstream audience. Through his personal use, extensive research, and wide-ranging expertise in nutrition and health, Jonny sorts through the myriad home remedies from every discipline and tradition to show which work and how best to use these proven healing techniques. He also explains through approachable and articulate descriptions why they work and on what basis he selected these cures—whether it is patient testimonials or the latest scientific studies to give you peace of mind...
	Advanced Monitoring and Procedures for Small Animal Emergency and Critical Care by Jamie M. Burkitt Creedon (Editor), Harold Davis (Editor) Advanced Monitoring and Procedures for Small Animal Emergency and Critical Care is a comprehensive yet practical reference, providing hands-on information essential to veterinarians and veterinary technicians involved in emergency and critical care. Written by an expert team of veterinarians and veterinary technicians, this well-referenced book offers step-by-step protocols for performing advanced emergency and critical care procedures and monitoring techniques. Packed with practical guidance in an easy-to-use format, this book is ideally suited for quick access in emergency rooms or intensive care units.Organized primarily by body system, each chapter covers general principles, indications, equipment, techniques, basic interpretation, troubleshooting, and contraindications. Standardized...
	Comprehensive Gynecology: Expert Consult - Online and Print, 6e (Comprehensive Gynecology (Mishell/Herbst)) by Gretchen M Lentz MD (Author), Rogerio A. Lobo MD (Author), David M Gershenson MD (Author), Vern L. Katz MD (Author) Comprehensive Gynecology provides you with complete, timely, and easy access to vital information on all the medical and surgical issues affecting your practice. Whether you're a resident or practioner, you'll get all the practical, in-depth coverage you need to stay at the forefront of your field. Now in its sixth edition, this core reference, formerly edited by Drs. Droegemueller, Stenchever, Mishell and Herbst, continues to be your primary resource for in-depth and up-to-date information.Stay up to date with the latest gynecologic advances through clear writing, a clinical focus, and a focus on evidence-based practices. Prepare for the challenges you'll face in practice with a completely overhauled legal chapter containing factual scenarios. Search the full text online at...