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A novel method of isolating high quality RNA from Kupffer cells
April 17, 2009Kupffer cells, resident tissue macrophages that line the liver sinusoids, play a key role in modulating inflammation in a number of experimental models of liver injury. Since Kupffer cells represent only a small portion of the entire liver cell population, greatly outnumbered by the parenchymal cells, Kupffer cell isolation faces major technical obstacles. Laser capture microdissection (LCM) offers a method of isolating a single cell type from specific regions of tissue sections.
A research team led by Dr Stephen H Gregory from United States outlines isolation of Kupffer using LCM. This will be published on April 14, 2009 in the World Journal of Gastroenterology.
They found that mRNA extracted from the microdissected cell population displayed marked increases in colony stimulating factor-1 receptor and Kupffer cell receptor message expression, which demonstrated Kupffer cell enrichment. Gene expression by Kupffer cells derived from bile-duct-ligated, versus sham-operated, mice was compared. Microarray analysis revealed a significant (2.5-fold, q value < 10) change in 493 genes. Based on this fold-change and a standardized PubMed search, 10 genes were identified that were relevant to the ability of Kupffer cells to suppress liver injury
The methodology outlined herein provides an approach to isolating high quality RNA from Kupffer cells, without altering the tissue integrity.
World Journal of Gastroenterology
New lights on the pathogenic mechanisms of liver cirrhosis with ascites
The pathogenic mechanisms implicated in the failure of intestinal barrier in cirrhosis have not been fully elucidated as yet and remains to be investigated.
More Kupffer Cells Current Events and Kupffer Cells News Articles
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Proceedings of the 6th International Kupffer Cell Symposium: Cells of the Hepatic Sinusoid Held Aug 23-27, 1992 by Knook (Author), Wisse (Author) | |
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International Kupffer Cell Symposium: Kupffer Cells and Other Liver Sinusoidal Cells 1st: Proceedings by D.L. Knook (Editor), E. Wisse (Editor) | |
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Liver(Hepatic) Cells Kupffer Cells, X400 Photographic Poster Print, 24x18 by AllPosters.com AllPosters.com is the world's #1 seller of posters, prints, photographs, specialty products and framed art. We're dedicated to bringing our customers the best selection of high quality wall décor that is perfect for their home or office. Browse our catalog of over 300,000 items that include entertainment and specialty posters, decorative prints, and art reproductions. Whether you're looking for your favorite movie or music poster, a framed Monet reproduction, or a print of the Eiffel Tower you will find it at AllPosters.com. Visit our Amazon store today at www.amazon.com/allposters to find Special Offers and search by subject category or artist. AllPosters.com provides unmatched service with a 100% satisfaction guarantee. We ship internationally to over 80 countries. Decorate your... |
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Endotoxin and Kupffer cell activation in alcoholic liver disease.: An article from: Alcohol Research & Health by Michael D. Wheeler (Author) This digital document is an article from Alcohol Research & Health, published by U.S. Government Printing Office on December 22, 2003. The length of the article is 5191 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser. Citation Details Title: Endotoxin and Kupffer cell activation in alcoholic liver disease. Author: Michael D. Wheeler Publication: Alcohol Research & Health (Refereed) Date: December 22, 2003 Publisher: U.S. Government Printing Office Volume: 27 Issue: 4 Page: 300(7) Distributed by Thomson... | |
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Liver(Hepatic) Cells Kupffer Cells, X400 Photographic Poster Print, 16x12 by Art.com Art.com is the world's largest retailer of art prints, posters, photographs, and framed artwork. With our huge selection of over 400,000 prints, you'll easily find the perfect piece for your home, office, or classroom. Our art is printed on quality paper. When you order framed artwork, the piece is built by our team of in-house professionals. Visit our Amazon store today at www.amazon.com/artdotcom to find Special Offers and search for products based on 'Artist Name' and 'Subject Categories' such as Movie, Music, Vintage, TV, Children, Travel, Kitchen, Museum Art, Animals, Floral, Motivational, and Sports. Art.com is dedicated to providing you with high quality products and service by offering you 100% satisfaction guaranteed. We ship internationally to over 80 countries. Decorate your... |
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Proceedings of the 7th International Kupffer Cell Symposium: Cells of the Hepatic Sinusoid Held Sept. 4-6, 1994 by Wisse (Author), Knook (Author), Wake (Author) | |
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Liver(Hepatic) Cells Kupffer Cells, X400 Photographic Poster Print, 32x24 by Art.com Art.com is the world's largest retailer of art prints, posters, photographs, and framed artwork. With our huge selection of over 400,000 prints, you'll easily find the perfect piece for your home, office, or classroom. Our art is printed on quality paper. When you order framed artwork, the piece is built by our team of in-house professionals. Visit our Amazon store today at www.amazon.com/artdotcom to find Special Offers and search for products based on 'Artist Name' and 'Subject Categories' such as Movie, Music, Vintage, TV, Children, Travel, Kitchen, Museum Art, Animals, Floral, Motivational, and Sports. Art.com is dedicated to providing you with high quality products and service by offering you 100% satisfaction guaranteed. We ship internationally to over 80 countries. Decorate your... |
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Hepatocyte and Kupffer Cell Interactions by Timothy R. Billiar (Author), Ronald D. Curran (Author) Hepatocyte and Kupffer Cell Interactions presents a comprehensive discussion of historical and recent information regarding this diverse field of research. The role of Kupffer cells and hepatoctyes in normal physiology, nonseptic pathological states, and in sepsis is examined. Microanatomy and methods of experimental study are covered as well. In each of the book's chapters, the role of the Kupffer cell and hepatocyte interaction is placed in context with information on particular liver functions or disease states. Hepatocyte and Kupffer Cell Interactions is an essential reference for leukocyte specialists, gastroenterologists, immunologists, and other researchers working in this fascinating field. |
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Kupffer Cells, Liver, X400 Ingesting Carbon Particles Photographic Poster Print, 40x30 by Art.com Art.com is the world's largest retailer of art prints, posters, photographs, and framed artwork. With our huge selection of over 400,000 prints, you'll easily find the perfect piece for your home, office, or classroom. Our art is printed on quality paper. When you order framed artwork, the piece is built by our team of in-house professionals. Visit our Amazon store today at www.amazon.com/artdotcom to find Special Offers and search for products based on 'Artist Name' and 'Subject Categories' such as Movie, Music, Vintage, TV, Children, Travel, Kitchen, Museum Art, Animals, Floral, Motivational, and Sports. Art.com is dedicated to providing you with high quality products and service by offering you 100% satisfaction guaranteed. We ship internationally to over 80 countries. Decorate your... |
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