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Protein analysis methods, viral vectors featured in Cold Spring Harbor Protocols
May 05, 2009COLD SPRING HARBOR, N.Y. - Many proteins do not function by themselves as stand-alone units. Instead, multiple proteins associate to form larger structures called protein complexes. The May issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc5_09.dtl) features a set of methods that can be used to analyze protein complexes. An additional featured article details the generation of viral vectors for gene transfer.
"Systematic Monitoring of Protein Complex Composition and Abundance by Blue-Native PAGE," written by Harvey Millar and colleagues from the University of Western Australia, describes multiple experimental approaches using polyacrylamide gel electrophoresis (PAGE). Blue-native PAGE (BN-PAGE) allows a range of protein complexes to be visualized. When combined with sodium dodecyl sulfate PAGE (SDS-PAGE), the procedure can resolve the complexes and their subunits by their molecular weight. In conjunction with differential in-gel electrophoresis (DIGE), BN-PAGE can be used to quantify changes in protein complex abundance or subunit composition between different samples. The article presents detailed methodology for BN-PAGE, SDS-PAGE, and DIGE. It is freely available on the website for Cold Spring Harbor Protocols.
Genetically modified adenoviruses serve as one of the most versatile and efficient gene delivery systems in use today. Laboratories throughout the world use adenoviruses for the delivery of DNA to cells for basic science and for gene therapy applications. Unlike most other vectors, adenoviruses can infect post-mitotic cells, which makes them particularly useful as vectors for gene delivery into cells like neurons. In the May issue of Cold Spring Harbor Protocols, Robin Parks and colleagues from the Ottawa Health Research Institute provide "Construction and Characterization of Adenovirus Vectors," a set of detailed instructions for the generation, propagation, purification, and characterization of adenovirus vectors. This method is freely accessible on the website for Cold Spring Harbor Protocols.
Cold Spring Harbor Laboratory
Cold Spring Harbor Laboratory
Protein Complexes Current Events and Protein Complexes News RSSChromosomes dance and pair up on the nuclear membrane
Meiosis - the pairing and recombination of chromosomes, followed by segregation of half to each egg or sperm cell - is a major crossroads in all organisms reproducing sexually.
Structure of protective protein in the eye lens revealed
The human eye lens consists of a highly concentrated mix of several proteins. Protective proteins prevent these proteins from aggregating and clumping.
New computer simulation helps explain folding in important cellular protein
Most parts of living organisms come packaged with ribbons. The ribbons are proteins-chains of amino acids that must fold into three-dimensional structures to work properly.
GUMC discovery highlights new direction for drug discovery
In a discovery that rebuffs conventional scientific thinking, researchers at Georgetown University Medical Center (GUMC) have discovered a novel way to block the activity of the fusion protein responsible for Ewing's sarcoma, a rare cancer found in children and young adults.
Plant protein 'doorkeepers' block invading microbes, study finds
A group of plant proteins that "shut the door" on bacteria that would otherwise infect the plant's leaves has been identified for the first time by a team of researchers in Denmark, at the University of California, Davis, and at UC Berkeley.
New images may improve vaccine design for deadly rotavirus
Howard Hughes Medical Institute researchers are reporting the first detailed molecular snapshots of a deadly gastrointestinal virus as it is caught in the grasp of an immune system molecule with the capacity to destroy it.
Learning how the pieces responsible for interpreting the human genome work
The human genome complete sequencing project in 2003 revealed the enormous instruction manual necessary to define a human being. However, there are still many unanswered questions. There are few indications on where the functional elements are found in this manual.
Jefferson scientists discover a key protein regulator of inflammation and cell death
Reporting in the journal Nature, researchers led by Emad Alnemri, Ph.D., professor of Biochemistry and Molecular Biology in the Kimmel Cancer Center at Jefferson, discovered a key protein component involved in inflammation.
E. coli persists against antibiotics through HipA-induced dormancy
Bacteria hunker down and survive antibiotic attack when a protein flips a chemical switch that throws them into a dormant state until treatment abates, researchers at The University of Texas M. D. Anderson Cancer Center report in the Jan.16 edition of Science.
Interactive gene 'networks' may predict if leukemia is aggressive or slow-growing
Rather than testing for individual marker genes or proteins, researchers at the University of California, San Diego (UC San Diego) and the Moores UCSD Cancer Center have evidence that groups, or networks, of interactive genes may be more reliable in determining the likelihood that a form of leukemia is fast-moving or slow-growing.
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