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Faster, more cost-effective DNA test for crime scenes, disease diagnosis
July 09, 2009Analytical Chemistry
Scientists in Japan are reporting development of a faster, less expensive version of the fabled polymerase chain reaction (PCR) a DNA test widely used in criminal investigations, disease diagnosis, biological research and other applications. The new method could lead to expanded use of PCR in medicine, the criminal justice system and elsewhere, the researchers say. Their study is scheduled for the July 15 issue of Analytical Chemistry, a semi-monthly journal.
In the new study, Naohiro Noda and colleagues note that PCR works by "amplifying" previously undetectable traces of DNA almost like photocopiers produce multiple copies of documents. With PCR, crime scene investigators can change traces of DNA into amounts that can be identified and linked to a suspect. Biologists can produce multiple copies of individual genes to study gene function, evolution, and other topics. Doctors can amplify the DNA from microbes in a patient's blood to diagnose an infection. Current PCR methods, however, are too expensive and cumbersome for wide use.
The scientists describe development and testing of a new PCR method, called the universal QProbe system, that overcomes these problems. Existing PCR processes require several "fluorescent probes" to seek out DNA. QProbe substitutes a single "fluorescent probe" that can detect virtually any target, saving time and cutting costs. The new method also is more specific, accurately detecting DNA even in the presence of unfavorable PCR products in the samples that may interfere with quantification results.
American Chemical Society
American Chemical Society
Disease Diagnosis Current Events and Disease Diagnosis News RSSTracking down the human 'odorprint'
Each of the 6.7 billion people on Earth has a signature body odor - the chemical counterpart to fingerprints - and scientists are tracking down those odiferous arches, loops, and whorls in the "human odorprint" for purposes ranging from disease diagnosis to crime prevention.
Declines in other thinking and learning skills may precede memory loss in Alzheimer's disease
Cognitive abilities other than memory, including visuospatial skills needed to perceive relationships between objects, may decline years prior to a clinical diagnosis in patients with Alzheimer's disease, according to a report in the October issue of Archives of Neurology, one of the JAMA/Archives journals.
A better test to detect DNA for diagnosing diease, investigating crimes
Researchers in Singapore are reporting development of a new electronic sensor that shows promise as a faster, less expensive, and more practical alternative than tests now used to detect DNA.
New ultrasensitive electronic sensor array speeds up DNA detection
A novel electronic sensor array for more rapid, accurate and cost-efficient testing of DNA for disease diagnosis and biological research has been developed by scientists at Singapore's Institute of Bioengineering and Nanotechnology (IBN).
M. D. Anderson study finds strong relationship between high body mass index, pancreatic cancer
In reviewing the weight history of pancreatic cancer patients across their life spans, researchers at The University of Texas M. D. Anderson Cancer Center have determined that a high body mass index in early adulthood may play a significant role in an individual developing the disease at an earlier age.
Researchers make progress toward early identification of muscular dystrophy
The saying "Knowing is half the battle" is never more true than when discussing early treatment of disease. Muscular dystrophy is one such disease where patients can benefit from early treatment. Now, new research is moving doctors and scientists closer to disease diagnosis in advance of patient symptoms.
A quicker, cheaper SARS virus detector -- one easily customizable for other targets
Members of a USC-led research team say they've made a big improvement in a new breed of electronic detectors for viruses and other biological materials - one that may be a valuable addition to the battle against epidemics.
Ultrasound more cost efficient than other medical imaging choices
In comparing ultrasound with other medical imaging methods such as MRI and CT scans, a literature review of published studies in the May/June issue of Journal of Diagnostic Medical Sonography (JDMS) describes the use of ultrasound to provide an accurate diagnosis more cost effectively than the alternatives.
Singapore scientists synthesize gold to shed light on cells' inner workings
Highly fluorescent gold nanoclusters for sub-cellular imaging have been synthesized by researchers at the Institute of Bioengineering and Nanotechnology (IBN).
Is there a prospective association between obesity and periodontal disease?
This is the question asked by a team of investigators from the Harvard School of Public Health and the University of Puerto Rico, reporting their findings today during the 87th General Session of the International Association for Dental Research, convening at the Miami Beach Convention Center.
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