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Methods for gene transfer in stem cells featured in Cold Spring Harbor Protocols
August 06, 2009Vectors derived from retroviruses are useful tools for long-term gene transfer because they allow stable integration of transgenes and propagation into daughter cells. Lentiviral vectors are preferred because they can transduce non-proliferating cellular targets. These vectors can be engineered to target specific tissues.
In the August issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc8_09.dtl), François-Loïc Cosset and colleagues from Ecole Normale Supérieure de Lyon (http://hvd.ens-lyon.fr/human_virology_dpt) present a method for targeting hematopoietic stem cells using engineered viral vectors. The article, "Hematopoietic Stem Cell Targeting with Surface-Engineered Lentiviral Vectors," is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/8/pdb.prot5276).
Though viral vectors are highly efficient, their use can raise concerns about recombination, immune responses and other safety issues. DNA transposons offer an effective, alternative method for nonviral gene transfer that avoids the safety concerns associated with viral vectors. Use of the "Sleeping Beauty Transposon System for Stable Gene Expression in Mouse Embryonic Stem Cells" from Catherine Krull and colleagues at the University of Michigan (http://www.med.umich.edu/cdb/sub_pages/People/krull.htm) provides a method for stable integration and reliable long-term expression of a transgene. Sleeping Beauty transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat sequences that result in precise integration into a TA dinucleotide. The article is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/8/pdb.prot5270).
Cold Spring Harbor Laboratory
Cold Spring Harbor Laboratory
Gene Transfer Current Events and Gene Transfer News RSSMichigan hospital launches gene therapy study for Parkinson's disease
A Michigan hospital is embarking on a research study for advanced Parkinson's disease using a state-of-the-art treatment called gene transfer.
Genes key to staph disease severity, drug resistance found hitchhiking together
Scientists studying Staphylococcus bacteria, including methicillin-resistant S. aureus (MRSA), have discovered a potent staph toxin responsible for disease severity.
Nervous system may be culprit in deadly muscle disease
Brain may win out over brawn as the primary cause of breathing problems in children with a severe form of muscular dystrophy known as Pompe disease.
Novel vaccine approach offers hope in fight against HIV
A research team may have broken the stubborn impasse that has frustrated the invention of an effective HIV vaccine, by using an approach that bypasses the usual path followed by vaccine developers.
New tool isolates RNA within specific cells
A team of University of Oregon biologists, using fruit flies, has created a way to isolate RNA from specific cells, opening a new window on how gene expression drives normal development and disease-causing breakdowns.
Protein analysis methods, viral vectors featured in Cold Spring Harbor Protocols
Many proteins do not function by themselves as stand-alone units. Instead, multiple proteins associate to form larger structures called protein complexes.
Gene therapy for muscular dystrophy shows promise beyond safety
Researchers have cleared a safety hurdle in efforts to develop a gene therapy for a form of muscular dystrophy that disables patients by gradually weakening muscles near the hips and shoulders.
UI biologist studies ocean plant cell adaptation in climate change
How will plant cells that live in the oceans and serve as the basic food supply for many of the world's sea creatures react to climate change?
Gene therapy shows early promise for treating obesity
With obesity reaching epidemic levels, researchers at the Ohio State University Medical Center are studying a potentially long-term treatment that involves injecting a gene directly into one of the critical feeding and weight control centers of the brain.
Magnetic nanoparticles navigate therapeutic genes through the body
Health professionals send genes and healthy cells on their way through the bloodstream so that they can, for example, repair tissue damage to arteries.
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Gene Transfer: Delivery and Expression of DNA And RNA, A Laboratory Manual by Theodore Friedmann (Author), Theodore Friedmann (Editor), John Rossi (Editor) Understanding GENE FUNCTION and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer; Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring appropriate expression. The detailed, step by step protocols cover a variety of methods, both well established and newly evolving. These includeviral and... |
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Jukin' The Manhattan Transfer (Performer), Gene Pistilli (Performer) |
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An American in Paris [VHS] Starring: Gene Kelly, Leslie Caron, Oscar Levant, Georges Guétary, Nina Foch Directed By: Vincente Minnelli Also With: Alfred Gilks (Cinematographer), Adrienne Fazan (Editor), Arthur Freed (Producer), Roger Edens (Producer), Alan Jay Lerner (Writer) A GI (Gene Kelly) stays in Paris after the war to become an artist, and has to choose between the patronage of a rich American woman (Nina Foch) and a French gamine (Leslie Caron) engaged to an older man. The plot is mostly an excuse for director Vincente Minnelli to pool his own extraordinary talent with those of choreographer-dancer-actor Kelly and the artists behind the screenplay, art direction, cinematography, and score, creating a rapturous musical not quite like anything else in cinema. The final section of the film comprises a 17-minute dance sequence that took a month to film and is breathtaking. Songs include "'S Wonderful," "I Got Rhythm," and "Love Is Here to Stay." --Tom Keogh |
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Muscle Beach Party Starring: Frankie Avalon, Annette Funicello, Luciana Paluzzi, John Ashley, Don Rickles Directed By: William Asher Also With: Samuel Z. Arkoff (Producer), Robert Dillon (Producer), James H. Nicholson (Producer), William Asher (Writer), Robert Dillon (Writer) |
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Gene Transfer and the Ethics of First-in-Human Research: Lost in Translation by Cambridge University Press (Publisher) Human gene transfer is widely regarded as one of the most promising technologies for the treatment of a variety of disorders, but it presents practitioners with a variety of difficult ethical questions. Gene Transfer and the Ethics of First-in-Human Research examines the ethical and policy dimensions of testing interventions in human beings for the first time. The book discusses the difficult ethical challenges that arise from attempting to translate laboratory discoveries into clinical applications. These range from which available techniques to use, when to initiate human testing, questions of consent, expectation in public arenas, how to define acceptable risk, and the inclusion of vulnerable or disadvantaged subjects in early phase trials. This book is relevant to ethicists, legal... |
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Horizontal Gene Transfer in the Evolution of Pathogenesis (Advances in Molecular and Cellular Microbiology) by Michael Hensel (Editor), Herbert Schmidt (Editor) Horizontal gene transfer is a major driving force in the evolution of many bacterial pathogens. The development of high-throughput sequencing tools and more sophisticated genomic and proteomic techniques in recent years has resulted in a better understanding of this phenomenon. Written by leading experts in the field, this edited volume is aimed at graduate students and researchers and provides an overview of current knowledge relating to the evolution of microbial pathogenicity. This volume provides an overview of the mechanisms and biological consequences of the genome rearrangements resulting from horizontal gene transfer, in both prokaryotes and eukaryotes, as well as overviews of the key mobile genetic elements involved. Subsequent chapters focus on paradigms for the evolution of... |
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Immunology of Gene Therapy by Roland W. Herzog (Author) Summarises and reviews the important field of genetic therapy with respect to the latest immunological advances in the lab and clinic Unique treatment of immunology and immunotherapy in gene - approached from a vector and target organ point of view rather than from the perspective a specific diseases Broad appeal - applicable for immunology and genetics / gene therapy, recombinant DNA studies, transplantation, virology, cancer research and tumor research |
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