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Short, sharp shock treatment for E. coli
January 12, 2012
A short burst of low voltage alternating current can effectively eradicate E. coli bacteria growing on the surface of even heavily contaminated beef, according to a study published in the International Journal of Food Safety, Nutrition and Public Health. The technique offers an inexpensive and easy to implement approach to reducing the risk of food poisoning, which can occur despite handlers complying with hygiene standards. Food poisoning is a serious public-health issue, especially with the emergence of lethal and highly virulent strains of Escherichia coli (E. coli O157:H7, for example). Infection with this bacterium causes serious diarrhea, dehydration, kidney problems and can lead to serious long-term problems or even be fatal in children, the elderly and people with pre-existing health problems. Tens of thousands of people are affected by E. coli infection each year through eating contaminated beef and other food products. The US Centers for Disease Control and Prevention (CDC) estimates that about 2500 people are hospitalized and there are several dozen deaths each year. Now, Ajit Mahapatra and colleagues at Fort Valley State University, in Georgia and Virginia Tech have demonstrated that applying a low-voltage alternating current to beef samples inoculated with large numbers of the potentially lethal E. coli O157:H7 can almost completely deactivate the bacterium, which is usually present on the surface of contaminated meat. The team points out that the level of contamination used in their tests far exceeded the contamination that would be seen in commercial carcasses after slaughter. Previous researchers had demonstrated that electricity can kill bacteria effectively. The study by Mahapatra and colleagues proves efficacy against E. coli O157:H7 at low voltage and low alternating current. It offers a quick and easy way to decontaminate at-risk, but otherwise safe beef without recourse to microbicidal chemicals or other more complicated treatment processes. Inderscience Publishers Related E Coli Current Events and E Coli News ArticlesAttacking MRSA with metals from antibacterial claysIn the race to protect society from infectious microbes, the bugs are outrunning us. The need for new therapeutic agents is acute, given the emergence of novel pathogens as well as old foes bearing heightened antibiotic resistance. Study IDs key protein for cell death When cells suffer too much DNA damage, they are usually forced to undergo programmed cell death, or apoptosis. However, cancer cells often ignore these signals, flourishing even after chemotherapy drugs have ravaged their DNA. Using 'bacteria-eaters' to prevent infections on medical implant materialsThey're ba-ack! But in a new disease-fighting role. Viruses that infect and kill bacteria - used to treat infections in the pre-antibiotic era a century ago and in the former Soviet Union today - may have a new role in preventing formation of the sticky "biofilms" of bacteria responsible for infections on implanted medical devices. Early infant growth rate linked to composition of gut microbiotaThe composition of gut microbiota in a new-born baby's gut has been linked to the rate of early infant growth, reports research published this week in PLOS Computational Biology. Nanosponges soak up toxins released by bacterial infections and venomEngineers at the University of California, San Diego have invented a "nanosponge" capable of safely removing a broad class of dangerous toxins from the bloodstream - including toxins produced by MRSA, E. coli, poisonous snakes and bees. Clinging to crevices, E. coli thriveNew research from Harvard University helps to explain how waterborne bacteria can colonize rough surfaces-even those that have been designed to resist water. Same-day water pollution test could keep beaches open more oftenWith warm summer days at the beach on the minds of millions of winter-weary people, scientists are reporting that use of a new water quality test this year could prevent unnecessary beach closures while better protecting the health of swimmers. Scissor-like enzyme points toward treatment of infectious diseaseUT Southwestern Medical Center researchers report that a pathogen annually blamed for an estimated 90 million cases of food-borne illness defeats a host's immune response by using a fat-snipping enzyme to cut off cellular communication. New study identifies unique mechanisms of antibiotic resistanceAs public health authorities across the globe grapple with the growing problem of antibiotic resistance, Tufts University School of Medicine microbiologists and colleagues have identified the unique resistance mechanisms of a clinical isolate of E. coli resistant to carbapenems. Researchers divide enzyme to conquer genetic puzzleRice University researchers have found a way to divide and modify enzymes to create what amounts to a genetic logic gate. More E Coli Current Events and E Coli News Articles

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Microcosm: E. Coli and the New Science of Life (Vintage)
by Carl Zimmer (Author)
A Best Book of the YearSeed Magazine • Granta Magazine • The Plain-DealerIn this fascinating and utterly engaging book, Carl Zimmer traces E. coli's pivotal role in the history of biology, from the discovery of DNA to the latest advances in biotechnology. He reveals the many surprising and alarming parallels between E. coli's life and our own. And he describes how E. coli changes in real time, revealing billions of years of history encoded within its genome. E. coli is also the most engineered species on Earth, and as scientists retool this microbe to produce life-saving drugs and clean fuel, they are discovering just how far the definition of life can be stretched.
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Poisoned: The True Story of the Deadly E. Coli Outbreak That Changed the Way Americans Eat
by Jeff Benedict (Author)
"Your perfect beach book has arrived. With Poisoned, Jeff Benedict manages to deliver the full literary experience of a medico-legal thriller in a work of nonfiction that, fortuitously enough, could not be more relevant to recent headlines."—The New York TimesIn this riveting work of narrative nonfiction, award-winning journalist and best-selling author Jeff Benedict chronicles the events surrounding the biggest food-poisoning epidemic in US history and how this unprecedented crisis sparked public awareness about unsanitary practices in the fast food industry. Poisoned draws on access to confidential documents and exclusive interviews with the real-life characters at the center of the drama.Jeff Benedict is considered one of America's top nonfiction writers. He is the author of nine...
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E.coli 0157
by Mary Heersink (Author)
In this fast-paced, emotionally charged true-life medical drama which verges on science fiction, a mother fights to save her son from a deadly bacteria.
At camp, eleven-year-old Damian Heersink suddenly becomes ill. Within days, an infection ravages through his bloodstream and attaches his organs one by one. A confusion of diagnosis and treatment ensues. Damian is rushed into surgery seven times in the battle to save his life.
Meanwhile the killer microbe, which has attached Damian like the virus in Richard Preston's The Hot Zone, is the cause of epidemics elsewhere. Tough investigators and heroic doctors work around the clock trying to stop its advance.
E.coli 0157 is a powerful human drama about a mother waging a desperate fight for her child's life, grappling with...
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E. coli in Motion (Biological and Medical Physics, Biomedical Engineering)
by Howard C. Berg (Author)
Escherichia coli, commonly referred to as E. coli, has been the organism of choice for molecular genetics for decades. Its machinery and mobile behavior is one of the most fascinating topics for cell scientists. Scientists and engineers, not trained in microbiology, and who would like to learn more about living machines, can see it as a unique example. This cross-disciplinary monograph covers more than thirty years of research and is accessible to graduate students and scientists alike.
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My Beef with Meat: The Healthiest Argument for Eating a Plant-Strong Diet--Plus 140 New Engine 2 Recipes
by Rip Esselstyn (Author)
For the millions who are following a plant-based diet, as well as those meat-eaters who are considering it, MY BEEF WITH MEAT is the definitive guide to convincing all that it's truly the best way to eat! New York Times Bestelling author of The Engine 2 Diet and nutrition lecturer Rip Esselstyn, is back and ready to arm readers with the knowledge they need to win any argument with those who doubt the health benefits of a plant-based diet--and convince curious carnivores to change their diets once and for all. Esselstyn reveals information on the foods that most people believe are healthy, yet that scientific research shows are not. Some foods, in fact, he deems so destructive they deserve a warning label. Want to prevent heart attacks, stroke, cancer and Alzheimer's? Then learn the facts...
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E.coli serotype and some Cytokines associated with UC and IBD patients: E.coli Serotypes and some Cytokines associated with UC and IBS
by Ali R. Hasim (Author), Saad S. Hamim (Author), Awatif H. Issa (Editor)
Irritable Bowel Syndrome and Inflammatory Bowel Diseases , including Crohn's disease and Ulcerative colitis, are two of the leading causes of chronic intestinal conditions in the world. Ulcerative colitis as an Inflammatory bowel disease is characterized by strong activation of the mucosa associated immune system due to a complex interaction of genetic, immunological and environmental factors .It is well established that the composition of the intestinal microbial flora has an impact on host immunity and influences the coarse of mucosal inflammation .Several immunological mucosal abnormalities have been described in ulcerative colitis patients; and these can be grouped into those that involve the epithelial barrier, those that involve the innate immune response (nonspecific...
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E.coli: Molecular phylogeny and pathogenicity islands: Molecular phylogeny and pathogenicity islands of E.coli
by Mohammed Sabri (Author), Lamees Abdul-Razzak (Author)
Phylogeny is the study of evolutionary relatedness among various groups of organism. Molecular phylogeny has also revealed that horizontal transfer plays an important and unexpected role in evolution. Phylogenetic analysis has shown that E. coli strains fall into four main phylogenetic groups (A, B1, B2,and D). the phylogeny groups of E. coli are distributed into intestinal and extraintestinal. There are three markers used in the phylogenetic analysis of E. coli as follows:- chuA, yjaA, and TspE4.C2. The presence of pathogenicity islands is one of the most indicator of the possession of E. coli of virulence factors. Pathogenicity of E. coli has been correlated with the presence of gene encoding for one or more virulence factors including (adhesins P and S fimbriae), iron acquisition...
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Proof of Heaven: A Neurosurgeon's Journey into the Afterlife
by Eben Alexander (Author)
A SCIENTIST’S CASE FOR THE AFTERLIFE Thousands of people have had near-death experiences, but scientists have argued that they are impossible. Dr. Eben Alexander was one of those scientists. A highly trained neurosurgeon, Alexander knew that NDEs feel real, but are simply fantasies produced by brains under extreme stress. Then, Dr. Alexander’s own brain was attacked by a rare illness. The part of the brain that controls thought and emotion—and in essence makes us human—shut down completely. For seven days he lay in a coma. Then, as his doctors considered stopping treatment, Alexander’s eyes popped open. He had come back. Alexander’s recovery is a medical miracle. But the real miracle of his story lies elsewhere. While his body lay in coma, Alexander journeyed beyond this world...
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E. coli Gene Expression Protocols (Methods in Molecular Biology)
by Peter E. Vaillancourt (Editor)
Peter E. Vaillancourt presents a collection of popular and emerging methodologies that take advantage of E. coli's ability to quickly and inexpensively express recombinant proteins. The authors focus on two areas of interest: the use of E. coli vectors and strains for production of pure, functional protein, and the use of E. coli as host for the functional screening of large collections of proteins and peptides. Among the cutting-edge techniques demonstrated are those for rapid high-level expression and purification of soluble and functional recombinant protein and those essential to functional genomics, proteomics, and protein engineering.
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E.coli: Probiotic for Piglet Diarrhoea: Escherichia Coli K88+ post Weaning Diarrhoea
by Amit Setia Dr Denis O Krause (Author)
Aim of this study was to select environmental E. coli isolates that produced colicins against the swine pathogen E. coli K88+. In initial evaluation using a modified plate method with 18 colicinogenic E. coli constructs, colicins E3, E4, E5, E9, Ia, K and N were found to possess inhibitory activity against 12 ETEC K88+ strains. A total of 463environmental isolates from cattle rumen, cattle feces, pig feces and hog manure-amendedsoil were screened for colicin production by a modified plate test. Further,colicinogenic isolates were screened for five toxin genes LT, STa, STb, VT1 and VT2 as well as K88 (F4) fimbriae using PCR reactions. Fourteen non-pathogenic isolates weresubjected to characterization of colicin genes by PCR using 9 new primer sequences, antibiotic susceptibilities and...
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