New Findings in Breast CancerApril 19, 2012
"It is fashionable to think of breast cancer as a disease that can be treated if cancer stem cells are killed off, but we are reporting a sub-population of cancer cells that have no obvious stem cell properties and yet can be tumorigenic and highly aggressive," Bissell says. "The existence of these additional sub-populations of tumorigenic cells tells us that the entire tumor needs to be eradicated, not just one sub-population of basal-like cells."
Bissell is a leading authority on breast cancer and a Distinguished Scientist with Berkeley Lab's Life Sciences Division. Petersen is a professor of Tissue Morphogenesis and Differentiation for CU's Cellular and Molecular Medicine department and a principal investigator for the Danish Stem Cell Center. They are the corresponding authors of a paper in the Proceedings of the National Academy of Sciences that describes this work. The paper is titled "Tumor initiating but differentiated luminal-like breast cancer cells are highly invasive in the absence of basal-like activity." Other co-authors were Jiyoung Kim, René Villadsen, Therese Sørlie, Louise Fogh, Signe Grønlund, Agla Fridriksdottir, Irene Kuhn, Fritz Rank, Vera Timmermans Wielenga, Hiroko Solvang, Paul Edwards, Anne-Lise Børresen-Dale and Lone Rønnov-Jessen.
"Our findings were a surprise to us as we had hypothesized the opposite scenario," Petersen says. "We wanted to learn more about possible tumor suppressive properties so we set out to characterize the bulk of the tumor, which we assumed would not be tumor-initiating."
Most breast cancers originate in the milk ducts and lobules, which consist of luminal cells surrounded by basal cells, and the two most frequent subtypes of human breast cancer cells are named luminal-like and basal-like after their resemblance to the two major lineages in the normal human breast. For the past several years, it has been widely believed that basal-like cells with the undifferentiated qualities of stem cells were the source of invasive tumors. However, since a majority of breast cancers exhibit luminal cell differentiation, the Petersen-Bissell collaboration investigated whether luminal-like differentiated cells within a basal-like cell hierarchy could also become tumorigenic, or must these luminal-like cells acquire basal-like traits to become malignant.
"We applied some rare and mutually exclusive lineage markers to isolate subsets of luminal-like and basal-like breast cancer cells, which enabled us to do single cell cloning with a reasonable success rate," Petersen says. "We enriched for populations with or without prominent basal-like traits from individual tumors, and from single cell cloning of cell lines and recovered cells with a luminal-like phenotype."
What they discovered was that luminal-like cells without specific basal-like traits were fully capable of initiating tumors in laboratory test mice; in fact, the tumors generated by luminal-like cells were larger than those from basal-like cells. In addition, when tested in an invasion assay, these phenotypically pure luminal-like cells were more invasive than the basal-like cells.
"Our findings demonstrate that basal-like cells, as defined currently, are not a requirement for breast tumor aggressiveness and that within a single tumor there are multiple cells with tumorigenic potential," Bissell says. "This casts doubt on the current hypothesis of hierarchical or differentiative loss of tumorigenicity."
The marker used by the Petersen-Bissell collaboration to isolate the luminal-like cancer cells was milk mucin (MM), one of the glycans, the sugar molecules that are ubiquitous to cell surfaces and central to cellular signaling. Basal-like cancer cells were marked with the protein CD271. The MM glycan was detected by the antibody M18. The CD271 marker was detected by the ME20.4 antibody.
In their investigation, the collaborators found that the ability of the luminal-like breast cancer cells to form large invasive tumors depended on the expression of the glycoprotein gene GCNT1, which in turn is required for the MM glycan to be detected by the M18 antibody. The collaborators believe this finding might be of potential clinical importance for breast cancer as co-author Kuhn, a member of Bissell's research group, explains.
"There has been an increasing appreciation that glycans play an important role in malignant behavior and our work shows that inhibiting the activity of GCNT1 could prevent the invasive behavior of the aggressive luminal-like cancer cells if they express the MM epitope," she says.
Although Petersen emphasizes that any clinical implications of this work remains speculative at this time, the finding that multiple types of cells in breast cancers are capable of initiating invasive tumors, he says, could hold implications for future personalized medicine.
"While new avenues in breast cancer targeting have been strongly influenced by the concepts of tumor subtypes and cancer stem cells, we believe our data and recent data from other laboratories opens the possibility of targeting individual stages of differentiation of breast cancer cells within a tumor," Petersen says. "In that way personalized medicine in the future could rely on a cellular-composition-profile with each cellular component accounted for in the treatment design."
This research was supported by grants from the Danish Cancer Society, the Novo Nordisk Foundation, and the Danish Council for Strategic Research, and in part the National Cancer Institute, the breast cancer program of the U.S. Department of Defense, and the U.S. Department of Energy's Office of Science
Lawrence Berkeley National Laboratory
Related Breast Cancer Current Events and Breast Cancer News Articles
21-gene recurrence score and receipt of chemotherapy in patients with breast cancer
Use of the 21-gene recurrence test score was associated with lower chemotherapy use in high-risk patients and greater use of chemotherapy in low-risk patients compared with not using the test among a large group of Medicare beneficiaries, according to an article published online by JAMA Oncology.
Stiffer breast tissue in obese women promotes tumors
Women who are obese have a higher risk and a worse prognosis for breast cancer, but the reasons why remain unclear.
Use of tamoxifen by young women is influenced by fertility concerns
The risk of breast cancer recurrence and mortality is decreased by endocrine therapy (ET) such as tamoxifen, but younger patients may decline it or discontinue treatment early if they are concerned about fertility, according to a study published August 24 in the JNCI: Journal of the National Cancer Institute.
Blood vessel cells help tumours evade the immune system
A study by researchers at Sweden's Karolinska Institutet is the first to suggest that cells in the tumour blood vessels contribute to a local environment that protects the cancer cells from tumour-killing immune cells.
Study examines breast cancer mortality after ductal carcinoma in situ diagnosis
Researchers estimate the 20-year breast cancer-specific death rate for women diagnosed with ductal carcinoma in situ to be 3.3 percent, although the death rate is higher for women diagnosed before age 35 and for black women.
Ductal carcinoma in situ carries a higher risk of death than previously thought
Women diagnosed with ductal carcinoma in situ (DCIS) are twice as likely to die from breast cancer compared to the general U.S. population, according to a new study led by Dr. Steven Narod.
Tool boosts accuracy in assessing breast cancer risk
A national risk model that gauges a woman's chance of developing breast cancer has been refined to give a more accurate assessment.
Light/moderate drinking linked to increased risk of some cancers in women & male smokers
Even light and moderate drinking (up to one drink a day for women and up to two drinks a day for men) is associated with an increased risk of certain alcohol related cancers in women and male smokers, suggests a large study published by The BMJ today.
Drinking coffee daily may improve survival in colon cancer patients
Regular consumption of caffeinated coffee may help prevent the return of colon cancer after treatment and improve the chances of a cure, according to a new, large study from Dana-Farber Cancer Institute that reported this striking association for the first time.
First-of-its-kind study finds music therapy lowers anxiety during surgical breast biopsies
A first-of-its-kind study published in the Journal of Clinical Oncology finds that music therapy lessened anxiety for women undergoing surgical breast biopsies for cancer diagnosis and treatment. The two-year study out of University Hospitals Seidman Cancer Center involved 207 patients.
More Breast Cancer Current Events and Breast Cancer News Articles