Polyurethane phase morphology induces endothelial cell organization

January 05, 2016

A group of researchers from the Biomaterials and Regenerative Therapeutics Laboratory in the Department of Biomedical Engineering at University at Buffalo has shown that nanostructured phases of segmental polyurethanes can guide endothelial cells into networks which are critical for initiating vascular structures in regenerative tissue engineering applications. This study has provided an interesting avenue to guide cells with the nanoscale domains of synthetic matrix which has not been perceived as a matrix cue for endothelial cell organization.

Cell-material interactions play a key role in regulating cellular function and organization during tissue regeneration and therefore controlling these interactions on synthetic matrices are crucial for successful tissue engineering. Particularly, endothelial cells require synchronized cell-matrix and cell-cell interactions to organize into networks for capillary tube formation. In contrast to current approaches either utilizes nano and microscale topographies or immobilized cell adhesive ligand to control cellular organization, this study has shown that material induced solid state phase morphology of matrix can provide cues to endothelial cells to form networks, even without any biological stimuli.

The main concept herein describes the significance of segmental polyurethanes which forms nanostructured domains as phases owing to the inter- and intra-segmental interactions and these domains can guide the cells into networks. Using L-tyrosine based biodegradable and biocompatible polyurethanes, researchers have shown that polyurethane matrices display different phase morphologies depending on the segmental composition. The main concept was to utilize these nanodomains of polyurethane as matrix guided signal because natural extracellular matrix also exhibits self-assembled domains. When endothelial cells were cultured on 2-D polyurethane matrices, the cells organized into interconnected networks on polyurethane matrices which displayed well defined segregated phases in nanoscale dimensions. In comparison, cells on phase-mixed polyurethanes were not able to form organized structures. Endothelial cells can sense the nanoscale features of polyurethane matrix and the nanostructured phases provided contact-mediated adhesive guidance to the cells to form interconnected structures.

This concept is significant because material driven phases provided signals for endothelial organization in absence of biological matrix or growth factors and it demonstrates the role of nanophase morphology as a critical regulator for cellular organization.

The future goal is to further characterize the phase morphology with defined nanostructures through modulating polyurethane structure and composition, establishing relationships between varying phase morphology and endothelial cell response, and investigating these responses in 3D scaffolds. Application of this material driven phase engineering presents an exciting new direction for development of tissue engineered synthetic matrices where cellular responses can be tuned with precise spatial and temporal control.

Corresponding author for this study in TECHNOLOGY is Professor Debanjan Sarkar, Ph.D., debanjan@buffalo.edu.
-end-


World Scientific

Related Endothelial Cells Articles from Brightsurf:

JACC: BTS study looks at COVID-19's impact on cardiovascular tissue, endothelial cells
In the paper, ''Cardiorenal tissues express SARS-CoV-2 entry genes and basigin (BSG/CD147) increases with age in endothelial cells,'' publishing in JACC: Basic to Translational Research, researchers used publicly available gene expression data to determine the relative expression of key SARS-CoV-2 host entry/ processing genes in human cardiorenal tissues, including aorta, coronary artery, heart (atria and left ventricle), whole blood and the kidney and for comparison the colon, spleen and lung.

New way to target some rapidly dividing cancer cells, leaving healthy cells unharmed
Scientists at Johns Hopkins Medicine and the University of Oxford say they have found a new way to kill some multiplying human breast cancer cells by selectively attacking the core of their cell division machinery.

Nutrient deficiency in tumor cells attracts cells that suppress the immune system
A study led by IDIBELL researchers and published this week in the American journal PNAS shows that, by depriving tumor cells of glucose, they release a large number of signaling molecules.

Scientists modify CAR-T cells to target multiple sites on leukemia cells
In a preclinical study, scientists engineer new CAR-T cells to attack three sites on leukemia cells, instead of one.

Sphingotec's endothelial function biomarker bio-ADM® improves risk stratification of sepsis patients at ICUs
New study data show that monitoring blood levels of sphingotec's endothelial function biomarker bio-ADM® on top of guideline parameter lactate improves risk stratification of sepsis patients admitted to intensive care units.

sphingotec's endothelial function biomarker bio-ADM® predicts need for organ support in general ICU patient population
Data from more than 2,000 patients enrolled in the FROG-ICU study demonstrate that high levels of bioactive adrenomedullin (bio-ADM®) predict the need for organ support, ionotropes, and vasopressors in the general patient population at admission to the intensive care unit (ICU).

First-of-its-kind study in endothelial stem cells finds exposure to flavored e-cigarette liquids, e-cigarette use exacerbates cell dysfunction
There has been a rapid rise in e-cigarette use, but its health effects have not been well-studied and their effect on vascular health remains unknown.

Dead cells disrupt how immune cells respond to wounds and patrol for infection
Immune cells prioritise the clearance of dead cells overriding their normal migration to sites of injury.

Transplanted bone marrow endothelial progenitor cells delay ALS disease progression
Transplanting human bone marrow-derived endothelial progenitor cells into mice mimicking symptoms of amyotrophic lateral sclerosis (ALS) helped more motor neurons survive and slowed disease progression by repairing damage to the blood-spinal cord barrier, University of South Florida researchers report.

Revealed: How the 'Iron Man' of immune cells helps T cells fight infection
The immune system's killer T cells are crucial in fighting viral infections.

Read More: Endothelial Cells News and Endothelial Cells Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.