Peeking inside 'mini-brains' could boost understanding of the human brain

January 07, 2021

Geneva, Switzerland, 7 January 2021 - 'Mini-brains' are pin-head sized collections of several different types of human brain cell. They are used as a tool, allowing scientists to learn about how the brain develops, study disease and test new medicines. Personalized 'mini-brains' can be grown from stem cells generated from a sample of human hair or skin and could shed light on how brain disease progresses in an individual and how this person may respond to drugs.

Research published today by a team of scientists and engineers from HEPIA and the Wyss Center for Bio and Neuroengineering, in the journal Frontiers in Bioengineering and Biotechnology, has revealed the detailed internal anatomy of 'mini-brains', for the first time.

"Despite advances in growing 'mini-brains', it has been difficult to understand in detail what is going on inside - until now," said Professor Adrien Roux from the Tissue Engineering Laboratory, HEPIA, senior author on the paper.

"Typically, to look inside a 'mini-brain', we slice it thinly and view it on a slide under a microscope. This is a slow process that can damage the sample. Now, for the first time, we have produced high resolution 3D images of single neurons within intact 'mini-brains', revealing their remarkable complexity," added Dr Subashika Govindan, lead author on the paper, who carried out the work at HEPIA and is now Wellcome DBT early career fellow at the Indian Institute of Technology Madras (IITM).

The team combined a novel technique for labeling individual neurons with a method to make the whole sample completely transparent.

Leveraging the Wyss Center's microscopy capabilities, the team developed a state-of-the-art custom module, including a bespoke sample holder and sensitive imaging detectors, for capturing 3D images of entire intact 'mini-brains', without slicing them. They were then able to visualize and analyze the 3D morphology of specific neurons and their anatomical distribution inside the 'mini-brains'.

Dr Laura Batti, Microscopy Facility Manager at the Wyss Center said: "Human 'mini-brains' have a life span of more than a year and, with our new ability to visualize them in more detail, we can envision benefits such as reducing some animal testing."

The new approach could also enable imaging of large numbers of 'mini-brains', making it suitable for high-throughput screening for drug discovery or toxicity testing. It is reproducible and cost-effective and could potentially help accelerate personalized medicine studies.
-end-
The paper 'Mass generation, neuron labeling, and 3D imaging of minibrains' by Subashika Govindan, Laura Batti, Samira F. Osterop, Luc Stoppini and Adrien Roux is published in Frontiers in Bioengineering and Biotechnology. DOI: 10.3389/fbioe.2020.582650 https://wysscenter.ch/updates/peeking-inside-mini-brains-could-boost-understanding-of-the-human-brain-in-health-and-disease

About HEPIA

Apart from education and training, one of the missions of the HES (Schools of Applied Sciences) is to conduct applied research projects in conjunction with businesses, cultural institutions and health and welfare organisations, as well as research laboratories in their regions. These projects support technology transfers to economic, industrial and institutional partners.

Applied Research and Development (AR&D) activities at HEPIA are organised into four institutes, covering all the main areas of engineering and architecture, to carry out cross-disciplinary research projects.

Applied research also helps enhance the teaching delivered to students. HEPIA plays a flagship role in the development of innovative solutions and technologies and offers experience in practical projects, paving the way to a wealth of fascinating career prospects.

http://www.hesge.ch/hepia

About the Wyss Center for Bio and Neuroengineering, Geneva, Switzerland

The Wyss Center is an independent, non-profit research and development organization that advances our understanding of the brain to realize therapies and improve lives. The Wyss Center staff, together with the Center's academic, clinical and industrial collaborators, pursue innovations and new approaches in neurobiology, neuroimaging and neurotechnology. Wyss Center advances reveal unique insights into the mechanisms underlying the dynamics of the brain and the treatment of disease to accelerate the development of devices and therapies for unmet medical needs. The Wyss Center was established by a generous donation from the Swiss entrepreneur and philanthropist Hansjörg Wyss in 2014. Additional resources from funding agencies and other sources help the Wyss Center accelerate its mission. http://www.wysscenter.ch/

MEDIA CONTACT:

Wyss Center
Jo Bowler, Communications Manager
+41 (0) 58 201 03 09
johanna.bowler@wysscenter.ch

HEPIA
Prof. Dr Adrien Roux
adrien.roux@hesge.ch
+41 22 546 25 99

Video

Peeking inside 3D human 'mini-brains' to understand the brain https://youtu.be/TZC-LqtsUSM

Original video files are available to download from the Wyss Center on request.

Image downloads

A seven month old 3D 'mini-brain' imaged with a confocal microscope to reveal the structure of individual neurons throughout.
Image credit: HEPIA
Download 4MB

The Wyss Center's lightsheet microscope
Image credit: Wyss Center
Download 15MB

Wyss Center for Bio and Neuroengineering

Related Brain Articles from Brightsurf:

Glioblastoma nanomedicine crosses into brain in mice, eradicates recurring brain cancer
A new synthetic protein nanoparticle capable of slipping past the nearly impermeable blood-brain barrier in mice could deliver cancer-killing drugs directly to malignant brain tumors, new research from the University of Michigan shows.

Children with asymptomatic brain bleeds as newborns show normal brain development at age 2
A study by UNC researchers finds that neurodevelopmental scores and gray matter volumes at age two years did not differ between children who had MRI-confirmed asymptomatic subdural hemorrhages when they were neonates, compared to children with no history of subdural hemorrhage.

New model of human brain 'conversations' could inform research on brain disease, cognition
A team of Indiana University neuroscientists has built a new model of human brain networks that sheds light on how the brain functions.

Human brain size gene triggers bigger brain in monkeys
Dresden and Japanese researchers show that a human-specific gene causes a larger neocortex in the common marmoset, a non-human primate.

Unique insight into development of the human brain: Model of the early embryonic brain
Stem cell researchers from the University of Copenhagen have designed a model of an early embryonic brain.

An optical brain-to-brain interface supports information exchange for locomotion control
Chinese researchers established an optical BtBI that supports rapid information transmission for precise locomotion control, thus providing a proof-of-principle demonstration of fast BtBI for real-time behavioral control.

Transplanting human nerve cells into a mouse brain reveals how they wire into brain circuits
A team of researchers led by Pierre Vanderhaeghen and Vincent Bonin (VIB-KU Leuven, Université libre de Bruxelles and NERF) showed how human nerve cells can develop at their own pace, and form highly precise connections with the surrounding mouse brain cells.

Brain scans reveal how the human brain compensates when one hemisphere is removed
Researchers studying six adults who had one of their brain hemispheres removed during childhood to reduce epileptic seizures found that the remaining half of the brain formed unusually strong connections between different functional brain networks, which potentially help the body to function as if the brain were intact.

Alcohol byproduct contributes to brain chemistry changes in specific brain regions
Study of mouse models provides clear implications for new targets to treat alcohol use disorder and fetal alcohol syndrome.

Scientists predict the areas of the brain to stimulate transitions between different brain states
Using a computer model of the brain, Gustavo Deco, director of the Center for Brain and Cognition, and Josephine Cruzat, a member of his team, together with a group of international collaborators, have developed an innovative method published in Proceedings of the National Academy of Sciences on Sept.

Read More: Brain News and Brain Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.