Nav: Home

Scientists Studied bacterial cells in the photoemission spectrum

January 20, 2020

A team of Russian scientists working in cooperation with international colleagues used a new method combining visual microscopic observations and photoemission spectrum registration that can be used to create a map of the physical and chemical state of cell surface. The team studied the cells of the colon bacillus Escherichia coli that are a promising material for the development of nature-like technologies. The study was supported with a grant of the Russian Science Foundation, and the article about it was published in the Results in Physics journal.

Nature-like object studies are an actively developing field of science based on the use of biological materials. Among other things, it includes technologies for the development of nanosized constructions on the basis of biological macromolecules: DNA, protein capsules and conjugators, and nucleoprotein complexes. However, to create such devices scientists need to understand how a biological system functions as a whole, and also to have technologies of such constructions composition and structure fine tuning.

One of the best objects for the development of these technologies are the cells of the colon bacillus E. coli that can be easily cultivated in lab conditions. The bacteria produce ferritin-like proteins called Dps. One of their main functions in a cell is the accumulation of different iron compounds (of fixed shape and not more than 5 nm in size) inside the protein globule. Such molecules can be obtained using a long and relatively quite expensive method of extraction with different means of fractioning. Alternatively, the E. coli cells themselves can work as a factory for controlled production, formation, transportation, and distribution of these proteins with inorganic nuclei. However, the issues of physical and chemical state of iron compounds, as well as their local atomic and electronic constriction within the bacterial cells and on their surfaces remain open. Currently there are no universal direct experimental methods that are precise and chemically sensitive enough for the studies of microparticles on the surfaces of biological objects (e.g. cells).

A team of scientists from Voronezh State University together with their colleagues (including the representatives of Immanuel Kant Baltic Federal University) were the first to study bacterial cells using the PhotoEmission Electron Microscopy (PEEM) method. It allowed the researchers to visually observe individual E. coli cells and potentially to study their surfaces physical and chemical state. The team confirmed that PEEM was qualitatively applicable for this type of research.

"A team from Voronezh State University with the support of Russian Science Foundation used a set of high-resolution research methods including X-ray Photoelectron Spectroscopy and Scanning Electron Microscopy that confirmed the efficiency of the approach used. The results shown by the group hold out a hope for a wider use of PEEM for the bioimaging of cell objects with integrated inorganic nanoparticles. In other words, PEEM can be used to map inorganic inclusions on the cell surface, that is, to gain information on what atoms and in what state are localized on the membrane of a bacterial cell on the microscopic scale. X-ray Photoelectron Spectroscopy technique was applied with the use of the National Research Center Kurchatov Institute storage ring synchrotron radiation" said the head of the project Associate Professor Sergey Turishchev.

"In the future we plan to increase the resolving power of this approach in order to be able to obtain precise data about the surface of single cells or even specific areas on them. Moreover, we'd like to consider the application of this method not only to bacterial cells with quite resistant membranes, but also to eukaryotic cells," commented Sergey Antipov, Associate Professor, the head of the Molecular Biophysics and Bionanotechnologies science group at the School of Life Sciences Immanuel Kant Baltic Federal University.
-end-
The participants of the research represented Voronezh State University, Helmholtz-Zentrum Berlin for Materials and Energy (Berlin, Germany), Leibniz Institute of Photonic Technology (Jena, Germany), National Research Center Kurchatov Institute (Moscow), Institute for Cell Biophysics of the Russian Academy of Sciences (Pushchino), and Immanuel Kant Baltic Federal University (Kaliningrad).

Immanuel Kant Baltic Federal University

Related Science Articles:

75 science societies urge the education department to base Title IX sexual harassment regulations on evidence and science
The American Educational Research Association (AERA) and the American Association for the Advancement of Science (AAAS) today led 75 scientific societies in submitting comments on the US Department of Education's proposed changes to Title IX regulations.
Science/Science Careers' survey ranks top biotech, biopharma, and pharma employers
The Science and Science Careers' 2018 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.
Science in the palm of your hand: How citizen science transforms passive learners
Citizen science projects can engage even children who previously were not interested in science.
Applied science may yield more translational research publications than basic science
While translational research can happen at any stage of the research process, a recent investigation of behavioral and social science research awards granted by the NIH between 2008 and 2014 revealed that applied science yielded a higher volume of translational research publications than basic science, according to a study published May 9, 2018 in the open-access journal PLOS ONE by Xueying Han from the Science and Technology Policy Institute, USA, and colleagues.
Prominent academics, including Salk's Thomas Albright, call for more science in forensic science
Six scientists who recently served on the National Commission on Forensic Science are calling on the scientific community at large to advocate for increased research and financial support of forensic science as well as the introduction of empirical testing requirements to ensure the validity of outcomes.
World Science Forum 2017 Jordan issues Science for Peace Declaration
On behalf of the coordinating organizations responsible for delivering the World Science Forum Jordan, the concluding Science for Peace Declaration issued at the Dead Sea represents a global call for action to science and society to build a future that promises greater equality, security and opportunity for all, and in which science plays an increasingly prominent role as an enabler of fair and sustainable development.
PETA science group promotes animal-free science at society of toxicology conference
The PETA International Science Consortium Ltd. is presenting two posters on animal-free methods for testing inhalation toxicity at the 56th annual Society of Toxicology (SOT) meeting March 12 to 16, 2017, in Baltimore, Maryland.
Citizen Science in the Digital Age: Rhetoric, Science and Public Engagement
James Wynn's timely investigation highlights scientific studies grounded in publicly gathered data and probes the rhetoric these studies employ.
Science/Science Careers' survey ranks top biotech, pharma, and biopharma employers
The Science and Science Careers' 2016 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.
Three natural science professors win TJ Park Science Fellowship
Professor Jung-Min Kee (Department of Chemistry, UNIST), Professor Kyudong Choi (Department of Mathematical Sciences, UNIST), and Professor Kwanpyo Kim (Department of Physics, UNIST) are the recipients of the Cheong-Am (TJ Park) Science Fellowship of the year 2016.
More Science News and Science Current Events

Trending Science News

Current Coronavirus (COVID-19) News

Top Science Podcasts

We have hand picked the top science podcasts of 2020.
Now Playing: TED Radio Hour

Listen Again: The Power Of Spaces
How do spaces shape the human experience? In what ways do our rooms, homes, and buildings give us meaning and purpose? This hour, TED speakers explore the power of the spaces we make and inhabit. Guests include architect Michael Murphy, musician David Byrne, artist Es Devlin, and architect Siamak Hariri.
Now Playing: Science for the People

#576 Science Communication in Creative Places
When you think of science communication, you might think of TED talks or museum talks or video talks, or... people giving lectures. It's a lot of people talking. But there's more to sci comm than that. This week host Bethany Brookshire talks to three people who have looked at science communication in places you might not expect it. We'll speak with Mauna Dasari, a graduate student at Notre Dame, about making mammals into a March Madness match. We'll talk with Sarah Garner, director of the Pathologists Assistant Program at Tulane University School of Medicine, who takes pathology instruction out of...
Now Playing: Radiolab

What If?
There's plenty of speculation about what Donald Trump might do in the wake of the election. Would he dispute the results if he loses? Would he simply refuse to leave office, or even try to use the military to maintain control? Last summer, Rosa Brooks got together a team of experts and political operatives from both sides of the aisle to ask a slightly different question. Rather than arguing about whether he'd do those things, they dug into what exactly would happen if he did. Part war game part choose your own adventure, Rosa's Transition Integrity Project doesn't give us any predictions, and it isn't a referendum on Trump. Instead, it's a deeply illuminating stress test on our laws, our institutions, and on the commitment to democracy written into the constitution. This episode was reported by Bethel Habte, with help from Tracie Hunte, and produced by Bethel Habte. Jeremy Bloom provided original music. Support Radiolab by becoming a member today at Radiolab.org/donate.     You can read The Transition Integrity Project's report here.