Nicotine: The link between cigarette smoking and kidney disease progression?

January 29, 2007

(BETHESDA, MD) -- Cigarette smoke (CS) is the most preventable cause of death and chronic disease in the United States. In addition to being a risk factor for atherosclerosis and cancer, recent epidemiologic studies suggest that cigarette smoke promotes the progression of kidney disease.

The mechanisms by which cigarette smoke may accelerate some types of chronic kidney disease are currently unknown. A new study, being published by the American Physiological Society (http://www.the-aps.org/), demonstrates for the first time that human mesangial cells (MC) - cells in the blood vessels of the kidneys - are endowed with nicotinic receptors (nAChRs) á4, á5, á7, â2, â3, â4 and â5 (cells that interact with the nicotine in tobacco) and may play an active role in the development of certain kidney diseases.

The Study

The study, "Nicotine: The Link Between Cigarette Smoking and the Progression of Renal Injury?," was conducted by Edgar A. Jaimes, MD, Run-Xia Tian, MD, and Leopoldo Raij, MD, all of the Miller School of Medicine, University of Miami, Miami, FL. It appears in the Articles in Press Section of the American Journal of Physiology - Heart and Circulatory Physiology (http://ajpheart.physiology.org/), one of 11-peer reviewed journals published by the APS.

Overview of Methodology

Human mesangial cells were grown in CSC-Complete media, supplemented with 10 percent fetal calf serum. Cells were passed by trypsinization when confluent and used between the third and ninth passages. 3H-thymidine was used as an index of cell proliferation and cells were fasted for 72 hours in Maintenance Media and stimulated for 24 hours with nicotine (10-10 M to 10-7 M) or platelet derived growth factor.

Western blots were performed after the cells fasted and had been stimulated for 24 hours with nicotine (10-7). Cell homogenates were washed and thereafter centrifuged for 30 minutes. Blots were incubated overnight with one of nine antibodies, which included mouse anti-á2-nAChR mAb's, anti-á3-nAChR mAb's, or anti-á4-nAChR mAb's. Fibronectin mRNA expression was determined by real time PCR and protein expression was measured by western blot. Flow cytometry measurement was generated for reactive oxygen species.

Data were expressed as mean ± SEM. For statistical comparisons involving two groups, an unpaired Student t test was used. For comparisons involving more than two groups, ANOVA was used. Significance was considered when P<0.05.

Highlights of Results

Highlights of the results include the following: Summary and Conclusions

These studies are believed to be the first of their kind to: Recent epidemiologic studies demonstrate that cigarette smoking increases the risk for progressive chronic kidney disease, but the role of smoking in primary renal diseases is less known. The results of this research, coupled with earlier findings, reveal previously unrecognized mechanisms showing nicotine - a component of cigarette smoke - as an agent that may accelerate and promote the progression of chronic kidney disease.
-end-
JOURNAL PUBLICATION INFORMATION American Journal of Physiology - Heart and Circulatory Physiology, (Articles in Press) http://ajpheart.physiology.org/.

NOTE TO EDITORS To schedule an interview with a member of the research team, please contact Donna Krupa at 301.634.7209 (direct dial), 703.967.2751 (cell) or dkrupa@The-APS.org.

Physiology is the study of how molecules, cells, tissues and organs function to create health or disease. The American Physiological Society (APS) has been an integral part of this scientific discovery process since it was established in 1887.

American Physiological Society

Related Kidney Disease Articles from Brightsurf:

Waistline matters in kidney disease
Does fat matter in kidney disease? The investigators found that all measures of higher abdominal fat content (including visceral fat, liver fat, or subcutaneous fat) and slower walk times were associated with increased levels of cardiometabolic risk factors in adults with non-dialysis dependent kidney disease.

Reducing urinary protein for patients with rare kidney disease slows kidney decline
New findings show that reducing the amount of protein in the urine of patients with focal segmental glomerulosclerosis can significantly slow declines in kidney function and extend time before patients' kidneys fail.

Antioxidant agent may prevent chronic kidney disease and Parkinson's disease
Researchers from Osaka University developed a novel dietary silicon-based antioxidant agent with renoprotective and neuroprotective effects.

Acute kidney injury and end stage kidney disease in severe COVID-19
Many COVID-19 patients experience hematuria, proteinuria and elevated serum creatinine concentration early in the course of the disease.

Genes tell a story about diabetic kidney disease
Studying Finnish genes leads to unique revelations about the development of a serious complication of diabetes, and informs an ongoing genomic study of a Singaporean cohort as part of Singapore's Diabetes Study in Nephropathy and other Microvascular Complications (DYNAMO).

New study provides insight into chronic kidney disease
Researchers have further analyzed a known signaling pathway they believe brings them one step closer to understanding the complex physiology of patients with chronic kidney disease (CKD), which might provide a path to new treatment options.

Predicting risk of chronic kidney disease
Data from about 5 million people (with and without diabetes) in 28 countries were used to develop equations to help identify people at increased five-year risk of chronic kidney disease, defined as reduced estimated glomerular filtration rate (eGFR).

A healthy diet may help prevent kidney disease
In an analysis of published studies, a healthy dietary pattern was associated with a 30% lower incidence of chronic kidney disease.

Is kidney failure a man's disease?
A new analysis of the ERA-EDTA Registry [1] reveals a striking gender difference in the incidence and prevalence of end-stage renal disease.

Chronic kidney disease: Everyone's concern
850 million people worldwide are affected by kidney disease. This worrying figure was published last June.

Read More: Kidney Disease News and Kidney Disease Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.
Can't connect to localhost. Errorcode: 1203