New stem cell therapy in dogs -- a breakthrough in veterinary medicine

February 03, 2021

Dogs have been faithful human companions ever since their domestication thousands of years ago. With various improvements in veterinary medicine in the past decades, their life expectancy has increased. However, an unfortunate side effect of this longevity, much like in humans, has been an increase in the occurrence of chronic and degenerative conditions.

In humans, modern efforts to fight such diseases have culminated in the development of regenerative therapies, largely based on stem cells. These "baby" cells have the potential to differentiate and mature into many specialized cell types-- called "pluripotency." By transplanting stem cells and guiding their differentiation into desired cell types, researchers are effectively able to regenerate damaged tissues, thereby reversing the course various complex diseases. Although this technology is widely studied in humans, the potential for stem cell therapy in dogs is lacking.

To this end, a research team from Japan, led by Associate Professor Shingo Hatoya from Osaka Prefecture University, has been working on isolating "induced pluripotent stem cells" (iPSCs) from canine blood samples. iPSCs are a type of stem cell that can be "programmed" from a developed (or "differentiated") cell by introducing a specific set of genes into them. These genes code for proteins called "transcription factors," which induce the change from a differentiated to a pluripotent stem cell, which then have the ability to mature into various cell types. iPSCs can proliferate very rapidly, providing a reliable supply of suitable stem cells for regenerative therapies. "We successfully established an efficient and easy generation method of canine iPSCs from peripheral blood mononuclear cells" explains Dr. Hatoya. He highlights the significance of these findings for veterinary science, stating he hopes that in the near future, "it may be possible to perform regenerative medicinal treatments in dogs." These findings were published in the journal Stem Cells and Development.

The previous attempts by these scientists to generate iPSCs from canine blood cells, using viral "vectors" to deliver the pluripotency-inducing transcription factors, were not as effective as hoped. Therefore, in this study, they tested a different combination of inducing factors, which they believe were key to harvesting the full potential of these cells. Most importantly, the researchers needed to control how the reprogrammed cells replicated in the host body. Viral vectors that encode pluripotency-inducing transcription factors can be used to infect cells obtained from the blood and convert them into iPSCs; however, the researchers needed to be cautious: because these vectors integrate into the host genome, re-expression of these pluripotency factors in the host cell can cause tumor formation when these cells are transplanted in patients. To avoid this, the team developed "footprint-free" stem cells by using a particular type of viral vector that can generate iPSCs without genomic insertion and can be automatically "silenced" via "microRNAs" expressed by the cells. Then, they grew these cells in a special type of medium that contained various factors enhancing their pluripotency (including a "small-molecule cocktail"). Indeed, these cells grew and successfully developed germ layers (which form the basis of all organs).

Fascinatingly, these findings have paved the way for an easy stem cell therapy technique for man's best friends. "We believe that our method can facilitate the research involving disease modeling and regenerative therapies in the veterinary field," says Dr. Hatoya. Furthermore, the authors also believe that additional research into regenerative therapies for canines might have some ripple effects for human medicine. "Dogs share the same environment as humans and spontaneously develop the same diseases, particularly genetic diseases."

Translating findings from one field to another might mean veterinarians are able to find treatments, maybe even cures, for some of the diseases that still plague humanity.
-end-
About Osaka Prefecture University, Japan

Osaka Prefecture University (OPU) is one of the largest public universities in Japan.

OPU comprises three campuses, with a main campus in Sakai, Osaka. With four colleges for undergraduate students and seven graduate schools, the university offers stellar education in a myriad of fields like engineering, life and environmental sciences, science, economics, humanities and social sciences, and nursing. Not just this, the university also houses various international students, who can enrich their lives with opportunities for internships and exchange programs.

In April 2022, OPU will unite with Osaka City University (OCU) to form Japan's largest public university, University of Osaka (tentative name: undergoing approval).

For more details, please visit:

OPU: https://www.osakafu-u.ac.jp/en

University of Osaka (tentative name): https://u-of-osaka.jp/

About Associate Professor Shingo Hatoya from Osaka Prefecture University

Dr. Shingo Hatoya is an Associate Professor at the Department of Advanced Pathobiology of the Graduate School of Life and Environmental Science at the Osaka Prefecture University. Holding both PhD and DVM degrees, he specializes in research on canine and feline stem cells and their applications in regenerative therapies in veterinary medicine.

Osaka Prefecture University

Related Stem Cells Articles from Brightsurf:

SUTD researchers create heart cells from stem cells using 3D printing
SUTD researchers 3D printed a micro-scaled physical device to demonstrate a new level of control in the directed differentiation of stem cells, enhancing the production of cardiomyocytes.

More selective elimination of leukemia stem cells and blood stem cells
Hematopoietic stem cells from a healthy donor can help patients suffering from acute leukemia.

Computer simulations visualize how DNA is recognized to convert cells into stem cells
Researchers of the Hubrecht Institute (KNAW - The Netherlands) and the Max Planck Institute in Münster (Germany) have revealed how an essential protein helps to activate genomic DNA during the conversion of regular adult human cells into stem cells.

First events in stem cells becoming specialized cells needed for organ development
Cell biologists at the University of Toronto shed light on the very first step stem cells go through to turn into the specialized cells that make up organs.

Surprising research result: All immature cells can develop into stem cells
New sensational study conducted at the University of Copenhagen disproves traditional knowledge of stem cell development.

The development of brain stem cells into new nerve cells and why this can lead to cancer
Stem cells are true Jacks-of-all-trades of our bodies, as they can turn into the many different cell types of all organs.

Healthy blood stem cells have as many DNA mutations as leukemic cells
Researchers from the Princess Máxima Center for Pediatric Oncology have shown that the number of mutations in healthy and leukemic blood stem cells does not differ.

New method grows brain cells from stem cells quickly and efficiently
Researchers at Lund University in Sweden have developed a faster method to generate functional brain cells, called astrocytes, from embryonic stem cells.

NUS researchers confine mature cells to turn them into stem cells
Recent research led by Professor G.V. Shivashankar of the Mechanobiology Institute at the National University of Singapore and the FIRC Institute of Molecular Oncology in Italy, has revealed that mature cells can be reprogrammed into re-deployable stem cells without direct genetic modification -- by confining them to a defined geometric space for an extended period of time.

Researchers develop a new method for turning skin cells into pluripotent stem cells
Researchers at the University of Helsinki, Finland, and Karolinska Institutet, Sweden, have for the first time succeeded in converting human skin cells into pluripotent stem cells by activating the cell's own genes.

Read More: Stem Cells News and Stem Cells Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.