Organoids grown from bile duct cells repair human livers; may aid liver transplant processes

February 18, 2021

Organoids grown from bile duct epithelial cells can be used to repair damaged bile ducts in transplanted human livers, researchers report. The results provide proof of concept for using ex vivo cell-based therapy to improve organ function before transplantation, which could ultimately increase the number of useable organs on the transplant waiting list. Bile produced in the liver is carried to the small intestine through a network of bile ducts formed by biliary epithelial cells known as cholangiocytes. While crucial for digestion, bile becomes toxic when it accumulates in the liver. As a result, chronic liver diseases that affect cholangiocytes often result in liver failure and account for a significant number of human liver transplants. Though liver donors are almost always in short supply, organoid technology holds great promise for regenerative medicine and is often considered as a potential alternative to liver transplantation for biliary diseases. However, in vivo engraftment, survival and function of organoids in humans has yet to be established. Using single-cell RNA sequencing and a mouse model of biliary injury, Fotios Sampaziotis and colleagues found that organoids grown from primary human cholangiocytes retain their plasticity, allowing cells from one region to repair different regions of the biliary tree. Sampaziotis et al. transplanted cholangiocyte organoids into the intrahepatic ducts of deceased human donor livers undergoing normothermic perfusion - a technique used to keep organs alive outside the human body - and found that the engrafted organoids repaired bile ducts in the ex vivo livers. The findings provide proof of concept that cholangiocytes from non-diseased areas, such as the gallbladder, could be used in cell-based therapies for cholangiopathies affecting bile ducts within the liver. The findings bring "cell therapy for intrahepatic biliary disease a step closer to clinical translation," write Simone Kurial and Holder Willenbring in a related Perspective.
-end-


American Association for the Advancement of Science

Related Transplantation Articles from Brightsurf:

A revolutionary new treatment alternative to corneal transplantation
A new approach in ophthalmology that offers a revolutionary alternative to corneal transplantation has just been developed by researchers and clinicians in North America, Europe, and Oceania.

Fewer complications after organ transplantation
A large international study coordinated by University Hospital Regensburg and Charité - Universitätsmedizin Berlin has demonstrated the safety of new cell therapy approaches for use in kidney transplant recipients.

Elderly patients also benefit from kidney transplantation
So far, kidney transplantation has generally not been offered to elderly patients (>75 years) because of the perioperative risks.

New material will allow abandoning bone marrow transplantation
Scientists from the National University of Science and Technology 'MISIS' developed nanomaterial, which will be able to restore the internal structure of bones damaged due to osteoporosis and osteomyelitis.

Fewer medical tests -- timely listing for transplantation
Younger patients would benefit greatly from kidney transplantation. Their expected remaining lifetime may even be doubled by having a transplant.

Uterus transplantation -- ethically just as problematic as altruistic surrogacy
In 2014, the first child to have been gestated in a donated uterus was born.

Advancing transplantation: Hepatitis C-infected organs safe for transplantation when followed by antiviral treatment
Twenty patients at Penn Medicine have been cured of the hepatitis C virus (HCV) following lifesaving kidney transplants from deceased donors who were infected with the disease, according to a study published today in Annals of Internal Medicine.

Transplantation followed by antiviral therapy cured hepatitis C
Twenty patients who received kidneys transplanted from hepatitis C virus (HCV)-infected donors experienced HCV cure, good quality of life, and excellent renal function at one year.

The Journal of Heart and Lung Transplantation: 50 years of heart transplantation progress
This month marks the 50th anniversary of the world's first human heart transplant performed at Groote Schuur Hospital in Cape Town by South African surgeon, Christiaan Barnard.

Older donor lungs should be considered for transplantation
With a scarcity of lungs available for transplantation, the use of lungs from donors older than age 60 has been shown to achieve reasonable outcomes and should be considered as a viable option, according to research published online today in The Annals of Thoracic Surgery.

Read More: Transplantation News and Transplantation Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.