Right place, right time

February 19, 2020

The brain is a ravenous organ. A three-pound adult human brain consumes about a fifth of the body's energy, yet it cannot store energy on its own and requires constant nourishment from the cardiovascular system. The organ's energy needs fluctuate greatly depending on neural activity, and sufficient blood must be delivered to the right place at precisely the right time to ensure healthy brain function.

To meet these ever-shifting demands, a process known as neurovascular coupling rapidly increases blood flow to areas of heightened neural activity. This process is impaired in conditions such as hypertension, diabetes and Alzheimer's disease, and also serves as the foundation for imaging technologies such as fMRI, which uses blood flow as a readout for brain activity.

Despite its importance, it was unclear how the brain and blood vessels communicate to enable neurovascular coupling. Now, in a study published in Nature on Feb. 19, Harvard Medical School neuroscientists report the discovery of a control mechanism in the brains of mice that ensures adequate blood flow to areas of heightened neural activity in a rapid and precise manner.

Their experiments reveal that arteries in the brain actively regulate neurovascular coupling in response to neural activity, and that the protein Mfsd2a, previously implicated as a key regulator of the protective blood-brain barrier, is critical for this process. The findings shed light on mechanisms that enable new avenues of study into the role of neurovascular coupling in neurological diseases.

"We now have a firm handle on the mechanisms involved in neurovascular coupling, including its molecular, cellular and subcellular components, which we've never had before," said senior study author Chenghua Gu, professor of neurobiology in the Blavatnik Institute at HMS and a Howard Hughes Medical Institute faculty scholar.

"This puts us in a position to dissect this process and determine, for example, whether the neurovascular coupling impairments that we see in diseases like Alzheimer's are the result of a pathology or the cause," Gu said.

Tickling whiskers

In previous studies, Gu and colleagues demonstrated that the protective integrity of the blood-brain barrier is ensured by the protein Mfsd2a, which suppresses the formation of caveolae--small lipid bubbles containing signaling molecules--from capillaries in the brains of mice.

To their surprise, they found that arteries, which carry nutrient-rich blood from the lungs and account for around five percent of the blood vessels in the brain, had the opposite characteristics as capillaries. Arteries lacked Mfsd2a and exhibited high amounts of caveolae.

In the current study, the team investigated this observation. Spearheaded by co-first authors Brian Chow and Vicente Nunez, HMS research fellows in neurobiology, the researchers stimulated the whiskers of awake, healthy mice and simultaneously live-imaged the animals' brain activity using a powerful technique known as 2-photon microscopy.

In response to whisker stimulation, normal mice showed increased neural activity, arterial diameter and blood flow in the corresponding sensory area of the brain. However, mice genetically engineered to lack caveolae had the same neural activity but significantly reduced blood flow and arterial dilation, indicating deficits in neurovascular coupling.

The team specifically blocked endothelial cells that make up the lining of arteries from forming caveolae, by forcing these cells to express the normally absent Mfsd2a. This again resulted in significant impairments to neurovascular coupling, demonstrating the importance of caveolae in the arteries.

Additional experiments demonstrated the unique role of arterial endothelial cells. It was previously known that neural activity relaxes the smooth-muscle cells that surround arteries, which leads to vessel dilation and increased blood flow. In contrast, the results of the current study revealed a different mechanism in which caveolae in arterial endothelial cells enable neurovascular coupling by relaying the signal to relax from neurons to smooth-muscle cells.

"For over a century, we've known that this phenomenon exists, where neural activity rapidly increases blood flow in a very local and temporally precise manner," Chow said. "But the mechanisms for how the nervous system talks to the vascular system to coordinate this event were largely unknown, and it was extremely surprising to find that arterial endothelial cells play such an active role in the process."

Precision consumption

The team also discovered that caveolae functioned independently of nitric oxide signaling--an important systemic pathway that regulates blood vessel dilation, famously targeted by medications like nitroglycerine for heart failure or sildenafil (Viagra) for erectile dysfunction.

When both caveolae and nitric oxide signaling were blocked, the team saw a complete absence of neurovascular coupling. Each mechanism appears to play an equally important but independent role in regulating blood flow in response to neural activity. This finding suggests that caveolae in arteries may be responsible for more precisely targeted increases in blood flow, whereas nitric oxide acts more broadly, the authors said.

Gu and colleagues are now investigating the exact composition of the signaling molecules contained within caveolae to better understand this process. The researchers said they hope that the newly revealed mechanistic underpinnings of neurovascular coupling will enable new experimental approaches to study the biology of this process and how it goes awry in disease.

"We've established a very powerful set of genetic tools that allow us to not only identify but manipulate the molecular mechanisms at the heart of neurovascular coupling," Gu said. "This is important given how many aspects of neurovascular coupling are still unclear."

"For example, even if increased local blood supply is impaired, the brain still has blood flow and oxygen. What is the impact of this on neurons? How does this affect brain function? And does it contribute to conditions like neurovascular dementia?" Gu said. "We are now in the position to perform rigorous science that could allow us to answer questions like these."
-end-
The study was supported by the National Institutes of Health (K99 NS102429, R37 NS046579, DP1 NS092473, P30 NS072030), a Quan Fellowship, the Jane Coffin Childs Memorial Fund, Fidelity Biosciences Research Initiative and a faculty scholar grant from the Howard Hughes Medical Institute.

Additional authors include Luke Kaplan, Adam J. Granger, Karina Bistrong, Hannah L. Zucker, Payal Kumar and Bernardo L. Sabatini.

Harvard Medical School

Related Science Articles from Brightsurf:

75 science societies urge the education department to base Title IX sexual harassment regulations on evidence and science
The American Educational Research Association (AERA) and the American Association for the Advancement of Science (AAAS) today led 75 scientific societies in submitting comments on the US Department of Education's proposed changes to Title IX regulations.

Science/Science Careers' survey ranks top biotech, biopharma, and pharma employers
The Science and Science Careers' 2018 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.

Science in the palm of your hand: How citizen science transforms passive learners
Citizen science projects can engage even children who previously were not interested in science.

Applied science may yield more translational research publications than basic science
While translational research can happen at any stage of the research process, a recent investigation of behavioral and social science research awards granted by the NIH between 2008 and 2014 revealed that applied science yielded a higher volume of translational research publications than basic science, according to a study published May 9, 2018 in the open-access journal PLOS ONE by Xueying Han from the Science and Technology Policy Institute, USA, and colleagues.

Prominent academics, including Salk's Thomas Albright, call for more science in forensic science
Six scientists who recently served on the National Commission on Forensic Science are calling on the scientific community at large to advocate for increased research and financial support of forensic science as well as the introduction of empirical testing requirements to ensure the validity of outcomes.

World Science Forum 2017 Jordan issues Science for Peace Declaration
On behalf of the coordinating organizations responsible for delivering the World Science Forum Jordan, the concluding Science for Peace Declaration issued at the Dead Sea represents a global call for action to science and society to build a future that promises greater equality, security and opportunity for all, and in which science plays an increasingly prominent role as an enabler of fair and sustainable development.

PETA science group promotes animal-free science at society of toxicology conference
The PETA International Science Consortium Ltd. is presenting two posters on animal-free methods for testing inhalation toxicity at the 56th annual Society of Toxicology (SOT) meeting March 12 to 16, 2017, in Baltimore, Maryland.

Citizen Science in the Digital Age: Rhetoric, Science and Public Engagement
James Wynn's timely investigation highlights scientific studies grounded in publicly gathered data and probes the rhetoric these studies employ.

Science/Science Careers' survey ranks top biotech, pharma, and biopharma employers
The Science and Science Careers' 2016 annual Top Employers Survey polled employees in the biotechnology, biopharmaceutical, pharmaceutical, and related industries to determine the 20 best employers in these industries as well as their driving characteristics.

Three natural science professors win TJ Park Science Fellowship
Professor Jung-Min Kee (Department of Chemistry, UNIST), Professor Kyudong Choi (Department of Mathematical Sciences, UNIST), and Professor Kwanpyo Kim (Department of Physics, UNIST) are the recipients of the Cheong-Am (TJ Park) Science Fellowship of the year 2016.

Read More: Science News and Science Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.