Unhealthy attachments

February 25, 2014

(Santa Barbara, Calif.) -- Using the surface forces apparatus and an atomic force microscope, researchers at UC Santa Barbara have taken a molecular approach to myelin membrane interactions, leading to insights into demyelinating diseases, such as multiple sclerosis. Their research is published in the Proceedings of the National Academy of the Sciences.

For a healthy nervous system, axons -- the long projections of our nerve cells that run throughout our bodies -- must be properly insulated. Much like conventional power cords need electrical insulators around the conducting wires for efficient and effective transfer of current, axons rely on multiple bilayers of myelin to maintain a rapid and optimal transfer of impulses between, for instance, brain and organ, or spinal cord and muscle. These bilayers are composed of lipids (fat molecules), protein and water.

"Basically, myelin is this multiple stacking of lipid bilayers," said Dong Woog Lee, a researcher in UCSB's Department of Chemical Engineering, and the study's lead author. "They need to be compact, and with very little water between the bilayers."

However, even the slightest change in the composition of these myelin bilayers to affect their ability to insulate axons, the researchers found. To observe and measure the characteristics and differences between healthy and diseased myelin bilayers, they studied the ability of these layers to adhere to each other.

On each of the two opposing surfaces of the surface forces apparatus -- a highly sensitive instrument that can measure interactions between membranes -- the researchers deposited a lipid bilayer on a mica substrate. Then they immersed the setup in a buffer solution containing myelin basic protein (MBP), a biomolecule commonly found in myelin that gives them adhesive properties and plays a role in maintaining the optimal structure of the myelin sheath. They brought the two bilayers close together, allowing them to stick to each other, and then pulled them apart, measuring the strength of the adhesion brought about by the MBP "glue" between the bilayers, and also the MBP's adsorption -- the ability of the MBP molecules to stick to the bilayers' surfaces. They performed this experiment with both healthy myelin and with "disease-like" myelin bilayers.

"A lipid bilayer simulating a normal or healthy myelin membrane adsorbs this protein much better than a lipid bilayer simulating a multiple sclerosis-type of myelin membrane," said UCSB researcher Kai Kristiansen, "meaning that the protein attaches more strongly to the lipid bilayer and can make two apposing lipid bilayers adhere more firmly to each other and at a smaller distance -- which is highly desirable for a well functioning myelin around a neuron."

One common characteristic of diseased myelin is swelling, due to various causes such as the autoimmune responses associated with MS and its variants, or in cases of infection or exposure to certain chemicals. Genetics also play a role in the health of myelin.

"When the disease progresses, people can see that they swell and eventually vesiculate, creating scars," said Lee. The MBP layer between the lipid bilayers also swells with water that seeps in between the double lipid layers. Instead of being a compact, molecule-thick film, the MBP layer becomes more gel-like.

"And since there's more water between the bilayers, their insulation property decreases," added Lee. From there, impulses slow down along the axon, or dissipate before they reach their destinations, causing paralysis and loss of function.

Disease-related changes in the lipid domain structures' size and distribution also causes irregular adsorption of MBP onto the lipid bilayers and weakens their adhesion properties, leading to lower nerve insulation. This in turn also leads to lower nerve insulation.

The molecular study of healthy and diseased myelin bilayers differs from conventional genetic, cellular or immunological approaches and may provide future insights into causes and mechanisms of demyelinating diseases.

The next step, according to Jacob Israelachvili, professor of chemical engineering and of materials at UCSB, is to develop a user-friendly bench-top instrument that could be used in hospitals and clinics to visualize the membranes of certain cells, both healthy and pathological, whose domain structure can be an indicator of the progression of a disease.

"We are currently planning a collaboration with a local hospital to provide us with such membranes, for example, from leukemic blood cells, that we would tag with a suitable fluorescent dye to enable this imaging," he said.
-end-


University of California - Santa Barbara

Related Multiple Sclerosis Articles from Brightsurf:

New therapy improves treatment for multiple sclerosis
A new therapy that binds a cytokine to a blood protein shows potential in treating multiple sclerosis, and may even prevent it.

'Reelin' in a new treatment for multiple sclerosis
In an animal model of multiple sclerosis (MS), decreasing the amount of a protein made in the liver significantly protected against development of the disease's characteristic symptoms and promoted recovery in symptomatic animals, UTSW scientists report.

Not all multiple sclerosis-like diseases are alike
Scientists say some myelin-damaging disorders have a distinctive pathology that groups them into a unique disease entity.

New therapeutic options for multiple sclerosis in sight
Strategies for treating multiple sclerosis have so far focused primarily on T and B cells.

Diet has an impact on the multiple sclerosis disease course
The short-chain fatty acid propionic acid influences the intestine-mediated immune regulation in people with multiple sclerosis (MS).

The gut may be involved in the development of multiple sclerosis
It is incompletely understood which factors in patients with multiple sclerosis act as a trigger for the immune system to attack the brain and spinal cord.

Slowing the progression of multiple sclerosis
Over 77,000 Canadians are living with multiple sclerosis, a disease whose causes still remain unknown.

7T MRI offers new insights into multiple sclerosis
Investigators from Brigham and Women's Hospital have completed a new study using 7 Tesla (7T) MRI -- a far more powerful imaging technology -- to further examine LME in MS patients

How to improve multiple sclerosis therapy
Medications currently used to treat multiple sclerosis (MS) can merely reduce relapses during the initial relapsing-remitting phase.

Vaccinations not a risk factor for multiple sclerosis
Data from over 12,000 multiple sclerosis (MS) patients formed the basis of a study by the Technical University of Munich (TUM) which investigated the population's vaccination behavior in relation to MS.

Read More: Multiple Sclerosis News and Multiple Sclerosis Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.