News tips from the Journal of Neuroscience

March 04, 2008

1. Transcriptional Regulation of Acetylcholinesterase
Shelley Camp, Antonella De Jaco, Limin Zhang, Michael Marquez, Brian De La Torre, and Palmer Taylor

Acetylcholinesterase (AChE) expression increases dramatically as myoblasts and neuroblasts differentiate respectively into myotubes and neurons, but the molecular regulators responsible for this switch have not been identified. By expressing the entire AChE gene (with successive deletions) in a myoblast cell line, Camp et al. have now identified a region necessary for upregulation of AChE in muscle cells. Remarkably, the regulatory region is not necessary for expression of AChE in neurons. Upregulation of AChE in muscles required the endogenous promoter as well as this regulatory region--which includes consensus binding sites for the muscle regulatory factors MyoD and MEF2--suggesting that the region constitutes an enhancesome. When the regulatory region was removed by homologous recombination in transgenic mice, AChE expression was normal in neurons but almost completely absent from muscles and neuromuscular junctions. This provides the first strong evidence that AChE at neuromuscular junctions is produced in the muscle rather than motor neurons.

2. Mosaic Analysis of Cerebellar Development
J. Sebastian Espinosa and Liqun Luo

Mosaic analysis with double markers is a recently developed technique that can be used to label the progeny of single precursor cells. Espinosa and Luo used this technique to examine the proliferation and differentiation of cerebellar granule cells. Initial work revealed that all the daughters of a single granule cell precursor extend axons in a single sublayer of the molecular layer. The authors now show that although proliferation of precursors generally slows during development, individual precursors and their progeny begin dividing rapidly (and symmetrically) just before differentiation. These neurons form a clonally related group that exits the cell cycle together. As these cells differentiate, their axons extend along the surface of the developing molecular layer, creating a cluster of axons in one sublayer. This is significant because axons in different sublayers synapse onto different interneurons and different regions of Purkinje cells, and therefore clonally related cells may also be functionally related.

3. Cannabinoid Effects on Threat Perception
K. Luan Phan, Mike Angstadt, Jamie Golden, Ikechukwu Onyewuenyi, Ana Popovska, and Harriet de Wit

Cannabinoid receptors are expressed at high levels in the amygdala, where they are thought to play a role in reducing anxiety and extinguishing fear. Although many experimenters report anxiolytic effects of D9-tetrahydrocannabinol (THC), the neural basis for these subjective effects has not been demonstrated in humans. To begin to address this question, Phan et al. used functional magnetic resonance imaging to examine activity in the amygdala when subjects viewed threatening (angry or fearful) or nonthreatening (happy) faces--a probe of reactivity to social threat. As expected, control subjects had more activity in the amygdala when viewing threatening faces than when viewing nonthreatening faces. The difference between threatening and nonthreatening faces was smaller in subjects who had taken THC. This effect depended mostly on decreased responses to threatening faces, but a small increase in responses to nonthreatening faces was also detected.

4. Conditional Model of Parkinson's Disease
Silke Nuber, Elisabeth Petrasch-Parwez, Beate Winner, Jürgen Winkler, Stephan von Hörsten, Thorsten Schmidt, Jana Boy, Melanie Kuhn, Huu P. Nguyen, Peter Teismann, Jörg B. Schulz, Manuela Neumann, Bernd J. Pichler, Gerald Reischl, Carsten Holzmann, Ina Schmitt, Antje Bornemann, Wilfried Kuhn, Frank Zimmermann, Antonio Servadio, and Olaf Riess

The pathological mediators of neurodegeneration in Parkinson's disease (PD) are unknown. One hypothesis postulates that neurodegeneration results from proteolytic stress due to accumulation and aggregation of misfolded or overexpressed proteins. In support of this, mutations and duplications of the α-synuclein gene are found in some PD families, and aggregates of α-synuclein in cytoplasmic inclusions (Lewy bodies) are a hallmark of PD. This week, Nuber et al. report the development of transgenic mice that conditionally overexpress α-synuclein. The mice showed progressive loss of motor and cognitive function and some degeneration of hippocampal neurons and of dopaminergic neurons in the substantia nigra. Although cell death occurred in the substantia nigra, levels were below statistical significance, and most was not apoptotic as is seen in PD. α-Synuclein aggregated but did not form Lewy bodies. Interestingly, turning off α-synuclein overexpression slowed the progression of motor dysfunction but did not alter cellular pathology.
Please click here for the current table of contents.

Society for Neuroscience

Related Neurons Articles from Brightsurf:

Paying attention to the neurons behind our alertness
The neurons of layer 6 - the deepest layer of the cortex - were examined by researchers from the Okinawa Institute of Science and Technology Graduate University to uncover how they react to sensory stimulation in different behavioral states.

Trying to listen to the signal from neurons
Toyohashi University of Technology has developed a coaxial cable-inspired needle-electrode.

A mechanical way to stimulate neurons
Magnetic nanodiscs can be activated by an external magnetic field, providing a research tool for studying neural responses.

Extraordinary regeneration of neurons in zebrafish
Biologists from the University of Bayreuth have discovered a uniquely rapid form of regeneration in injured neurons and their function in the central nervous system of zebrafish.

Dopamine neurons mull over your options
Researchers at the University of Tsukuba have found that dopamine neurons in the brain can represent the decision-making process when making economic choices.

Neurons thrive even when malnourished
When animal, insect or human embryos grow in a malnourished environment, their developing nervous systems get first pick of any available nutrients so that new neurons can be made.

The first 3D map of the heart's neurons
An interdisciplinary research team establishes a new technological pipeline to build a 3D map of the neurons in the heart, revealing foundational insight into their role in heart attacks and other cardiac conditions.

Mapping the neurons of the rat heart in 3D
A team of researchers has developed a virtual 3D heart, digitally showcasing the heart's unique network of neurons for the first time.

How to put neurons into cages
Football-shaped microscale cages have been created using special laser technologies.

A molecule that directs neurons
A research team coordinated by the University of Trento studied a mass of brain cells, the habenula, linked to disorders like autism, schizophrenia and depression.

Read More: Neurons News and Neurons Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to