BAT's novel vaping product shows minimal toxicity in laboratory tests

March 12, 2019

A series of in vitro toxicology tests provide evidence that British American Tobacco's novel vaping product produces greatly reduced mutagenicity, cytotoxicity and effects on wound healing as compared to cigarette smoke.

Scientists at BAT carried out tests to characterise the biological impact of a prototype e-cigarette. The tests assessed and compared the mutagenicity, genotoxicity, cytotoxicity and tumour-promoting potential of a reference cigarette (3R4F) and a novel e-cigarette with a unique aerosolisation technology.

Total particulate matter (TPM) from each was tested, following OECD testing guidelines, using the Ames bacterial reverse mutation assay; the mouse lymphoma assay; the in vitro micronucleus assay; the neutral red uptake cell viability assay; and the Bhas 42 cell transformation assay.

Unsurprisingly, TPM from cigarette smoke tested positive in all assays. In stark contrast, TPM from the novel vaping product failed to elicit a response, even at much higher doses.

The scientists then tested extreme doses (exposures) of aerosol from the novel e-cigarette using the cytotoxicity assay and a modified mutagenicity assay, but these tests also showed significantly reduced responses compared to the reference cigarette, confirming the TPM results.

"The vapour from the novel e-cigarette did not have an appreciable effect even at doses exceeding those of cigarette smoke," said Marianna Gaca, Head of Pre-Clinical Assessment at BAT. "This study further supports the growing consensus that e-cigarettes are significantly less toxic than cigarette smoke and that the novel aerosolisation technology introduced with this prototype is no different."

Further in vitro studies were conducted and included endothelial wound healing and the application of contemporary screening approaches to study over 12 different toxicity and oxidative stress endpoints relating to lung function. Where 3R4F inhibited wound repair in endothelial cells and resulted in positive responses for oxidative stress and 6 other toxicity endpoints, the novel e-cigarette showed little or no activity in any of the in vitro assays where it was assessed.

The results show that this novel vaping product has the potential to be a reduced risk product compared to cigarette smoking, though further longer-term studies are required to substantiate this potential.

R&D at British American Tobacco

Related Wound Healing Articles from Brightsurf:

Wound-healing biomaterials activate immune system for stronger skin
Researchers at Duke University and the University of California, Los Angeles, have developed a biomaterial that significantly reduces scar formation after a wound, leading to more effective skin healing.

'What wound did ever heal but by degrees?' delayed wound healing due to gene mutations
Scientists at Fujita Health University, Japan, have discovered how deficiencies of the IL-36Ra protein -- caused by mutations in the IL36RN gene -- delay wound healing via the flooding of the wound with several types of immune cells.

Wound-healing waves
How do cells in our bodies ask for directions? Without any maps to guide them, they still know where to go to heal wounds and renew our bodies.

A new approach to understanding the biology of wound healing
Researchers use discarded wound dressings as a novel and non-invasive way to study the mechanisms that promote healing.

New insights into wound healing
Research from a multidisciplinary team led by Washington University may provide new insights into wound healing, scarring and how cancer spreads

Towards improved wound healing -- Chemical synthesis of a trefoil factor peptide
The family of trefoil factor peptides brings hope to both research and industry to improve the treatment of chronic disorders.

Researchers say genetics may determine wound infection and healing
In a first-of-its-kind study, researchers have determined that genetics may play a role in how wounds heal.

Researchers develop microscopy technique for noninvasive evaluation of wound healing
The GSK Center for Optical Molecular Imaging at the University of Illinois' Beckman Institute has designed a new microscopy technique that can be used to study the progression of wound healing.

How tissues harm themselves during wound healing
Researchers from Osaka University discovered that increased expression of Rbm7 in apoptotic tissue cells results in the recruitment of segregated-nucleus-containing atypical monocytes, leading to tissue fibrosis.

Linking wound healing and cancer risk
When our skin is damaged, a whole set of biological processes springs into action to heal the wound.

Read More: Wound Healing News and Wound Healing Current Events is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to