Going beyond the genome

March 14, 2002

A collaboration of scientists from Yale University and Invitrogen Corporation (Carlsbad, CA) has completed the largest analysis, to date, of protein localization in any eukaryote. Published in the March 15th issue of Genes & Development, Dr. Michael Snyder and colleagues report on their effort to characterize the proteome of baker's yeast, Saccharomyces cerevisiae.

Although the S. cerevisiae genome sequence was completed in 1996, over 2000 of the protein products encoded by the 6000 yeast genes are yet to be functionally characterized.

Dr. Snyder and colleagues have taken the next step in this post-genomic era: large scale analysis of the proteins encoded by the genome - the proteome. Although the genome sequence provided vast arrays of genetic information, it provided only limited information about how the dynamic nature of the encoded protein products drives cellular processes.

All proteins (with the exception of some mitochondrial and chloroplast proteins) are synthesized on ribosomes in the cytosol, but depending upon their function, the proteins are transported to their specific place of action. Since subcellular localization is a strong indicator of protein function, Dr. Snyder and colleagues developed a high-throughput method to tag individual proteins and visualize their movements within living cells.

Dr. Snyder and colleagues successfully determined the subcellular localization of over 2700 yeast proteins. Using a combination of various approaches, including a statistical computer algorithm, the researchers were able to predict the localization of all 6100 yeast proteins. In doing so, Dr. Snyder and colleagues have provided insight into the potential function of nearly half of all previously uncharacterized yeast proteins.

This work represents a major advance in S. cerevisiae proteomics, but it promises to be just the tip of the iceberg in these post-genomic times.
Note: Dr. Snyder has posted this data on a searchable database available online at ygac.med.yale.edu.

Cold Spring Harbor Laboratory

Related Genome Articles from Brightsurf:

Genome evolution goes digital
Dr. Alan Herbert from InsideOutBio describes ground-breaking research in a paper published online by Royal Society Open Science.

Breakthrough in genome visualization
Kadir Dede and Dr. Enno Ohlebusch at Ulm University in Germany have devised a method for constructing pan-genome subgraphs at different granularities without having to wait hours and days on end for the software to process the entire genome.

Sturgeon genome sequenced
Sturgeons lived on earth already 300 million years ago and yet their external appearance seems to have undergone very little change.

A sea monster's genome
The giant squid is an elusive giant, but its secrets are about to be revealed.

Deciphering the walnut genome
New research could provide a major boost to the state's growing $1.6 billion walnut industry by making it easier to breed walnut trees better equipped to combat the soil-borne pathogens that now plague many of California's 4,800 growers.

Illuminating the genome
Development of a new molecular visualisation method, RNA-guided endonuclease -- in situ labelling (RGEN-ISL) for the CRISPR/Cas9-mediated labelling of genomic sequences in nuclei and chromosomes.

A genome under influence
References form the basis of our comprehension of the world: they enable us to measure the height of our children or the efficiency of a drug.

How a virus destabilizes the genome
New insights into how Kaposi's sarcoma-associated herpesvirus (KSHV) induces genome instability and promotes cell proliferation could lead to the development of novel antiviral therapies for KSHV-associated cancers, according to a study published Sept.

Better genome editing
Reich Group researchers develop a more efficient and precise method of in-cell genome editing.

Unlocking the genome
A team led by Prof. Stein Aerts (VIB-KU Leuven) uncovers how access to relevant DNA regions is orchestrated in epithelial cells.

Read More: Genome News and Genome Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.