Nav: Home

A moveable feast: Antibiotics give C. diff a nutrient-rich environment, no competition

March 28, 2018

Using a mouse model, researchers from North Carolina State University have found that antibiotic use creates a "banquet" for Clostridium difficile (C. diff), by altering the native gut bacteria that would normally compete with C. diff for nutrients. The findings could lead to the development of probiotics and other strategies for preventing C. diff infection.

C. diff is a harmful bacterium that can cause severe, recurrent and sometimes fatal infections in the gut. Although the bacteria are commonly found throughout our environment, C. diff infections primarily occur in patients who are taking, or who have recently finished taking, antibiotics.

"We know that antibiotics are major risk factors for C. diff infection because they alter the gut microbiota, or composition of bacteria in the gut, by eliminating the bacteria that are normally there," says Casey Theriot, assistant professor of infectious disease at NC State and corresponding author of a paper describing the research. "Our latest work suggests that the microbiota may provide natural resistance to C. diff colonization by competing with C. diff for nutrients in that environment; specifically, for an amino acid called proline."

Theriot and postdoctoral fellow Joshua Fletcher introduced C. diff to antibiotic-treated mice and monitored their gut environment at four intervals: 0, 12, 24, and 30 hours after introduction. They conducted metabolomic and RNA sequencing analysis of the gut contents and the C. diff at these time points to find out which nutrients the bacteria were "eating." Metabolomics allowed the team to trace the abundance of the nutrients in the gut, and RNA analysis indicated which genes in the C. diff were active in metabolizing nutrients.

The researchers found that the amount of proline in the gut decreased as the population of C. diff increased. Additionally, the amount of a proline byproduct called 5-aminovalerate also increased, indicating that C. diff was metabolizing the proline. The RNA analysis further confirmed C. diff's use of proline, as genes related to proline metabolism in C. diff increased during the early stages of colonization, when proline was abundant.

"We've been able to show that in the absence of competition C. diff is metabolizing proline and other amino acids in the mouse model, using it as fuel to survive and thrive," Theriot says. "Hopefully this information could lead to the development of better probiotics, or 'good' bacteria that can outcompete C. diff for nutrients in the gut. The ultimate goal is to control these bacteria in ways that don't rely solely on antibiotics."
The research appears in mSphere, and was funded by the National Institutes of Health (grants R35GM119438 and K01GM109236). Fletcher is first author. Animal studies and RNA sequencing were done at the University of Michigan. Metabolon, Inc. conducted the metabolomics analysis. Cristina Lanzas, associate professor of infectious disease at NC State, and postdoctoral fellow Samantha Erwin also contributed to the work.

Note to editors: An abstract of the paper follows.

"Shifts in the gut metabolome and Clostridium difficile transcriptome throughout colonization and infection in a mouse model"

DOI: 10.1128/mSphere.00089-18

Authors: Joshua Fletcher, Samantha Erwin, Cristina Lanzas, Casey Theriot, North Carolina State University

Published: mSphere


Antibiotics alter the gut microbiota and decrease colonization resistance against C. difficile, however the mechanisms driving colonization resistance are not well understood. Loss of colonization resistance against C. difficile due to antibiotic treatment is associated with alterations in the gut metabolome, specifically increases in nutrients that C. difficile can utilize for growth in vitro. To define the nutrients C. difficile requires for colonization and pathogenesis in vivo, we used a combination of mass spectrometry and RNA seq to model the gut metabolome and C. difficile transcriptome throughout an acute infection in a mouse model at the following time points 0, 12, 24, and 30 hours. We also performed multivariate-based integration of the omics data to define the signatures that were most important throughout colonization and infection. Here we show that amino acids, in particular proline and branched chain amino acids, and carbohydrates decrease in abundance over time in the mouse cecum, and that C. difficile gene expression is consistent with their utilization in vivo. This was also reinforced by the multivariate-based integration of the omics data where we were able to discriminate metabolites and transcripts that support C. difficile physiology between the different time points throughout colonization and infection. This study illustrates how important the availability of amino acids and other nutrients are for the initial stages of C. difficile colonization and progression of disease. Future studies identifying the source of the nutrients, and engineering bacteria capable of outcompeting C. difficile in the gut will be important for developing new targeted bacterial therapeutics.

North Carolina State University

Related Bacteria Articles:

Conducting shell for bacteria
Under anaerobic conditions, certain bacteria can produce electricity. This behavior can be exploited in microbial fuel cells, with a special focus on wastewater treatment schemes.
Controlling bacteria's necessary evil
Until now, scientists have only had a murky understanding of how these relationships arise.
Bacteria take a deadly risk to survive
Bacteria need mutations -- changes in their DNA code -- to survive under difficult circumstances.
How bacteria hunt other bacteria
A bacterial species that hunts other bacteria has attracted interest as a potential antibiotic, but exactly how this predator tracks down its prey has not been clear.
Chlamydia: How bacteria take over control
To survive in human cells, chlamydiae have a lot of tricks in store.
Stress may protect -- at least in bacteria
Antibiotics harm bacteria and stress them. Trimethoprim, an antibiotic, inhibits the growth of the bacterium Escherichia coli and induces a stress response.
'Pulling' bacteria out of blood
Magnets instead of antibiotics could provide a possible new treatment method for blood infection.
New findings detail how beneficial bacteria in the nose suppress pathogenic bacteria
Staphylococcus aureus is a common colonizer of the human body.
Understanding your bacteria
New insight into bacterial cell division could lead to advancements in the fight against harmful bacteria.
Bacteria are individualists
Cells respond differently to lack of nutrients.

Related Bacteria Reading:

Best Science Podcasts 2019

We have hand picked the best science podcasts for 2019. Sit back and enjoy new science podcasts updated daily from your favorite science news services and scientists.
Now Playing: TED Radio Hour

Jumpstarting Creativity
Our greatest breakthroughs and triumphs have one thing in common: creativity. But how do you ignite it? And how do you rekindle it? This hour, TED speakers explore ideas on jumpstarting creativity. Guests include economist Tim Harford, producer Helen Marriage, artificial intelligence researcher Steve Engels, and behavioral scientist Marily Oppezzo.
Now Playing: Science for the People

#524 The Human Network
What does a network of humans look like and how does it work? How does information spread? How do decisions and opinions spread? What gets distorted as it moves through the network and why? This week we dig into the ins and outs of human networks with Matthew Jackson, Professor of Economics at Stanford University and author of the book "The Human Network: How Your Social Position Determines Your Power, Beliefs, and Behaviours".