Nav: Home

Using chemistry to unlock the difference between cold- and hot-brew coffee (video)

April 02, 2020

WASHINGTON, April 2, 2020 -- Cold brew may be the hottest trend in coffee-making, but not a lot is known about how this process alters the drink's chemical characteristics. Scientists now report that the content of potentially health-promoting antioxidants in coffee brewed without heat can significantly differ from a cup of joe prepared with the same beans in the traditional way, particularly for dark roasts.

The researchers are presenting their results through the American Chemical Society (ACS) SciMeetings online platform.

A brand-new video on the research is available at

"This study can inform coffee enthusiasts about how they may want to craft their own coffee at home, based on science and on analytical chemistry," says Meghan Grim, an undergraduate student who worked on the project.

"After I first tried cold-brew coffee, I wanted to make it at home, but it didn't turn out too great," says Niny Z. Rao, Ph.D., the project's principal investigator. With her collaborator, Megan Fuller, Ph.D., Rao looked into the scientific literature to learn what she had done wrong. "It turns out there weren't any studies on cold-brew coffee at the time," Rao says, "so we decided to do some."

In the cold-brew process, ground coffee is mixed with room-temperature or colder water and steeped for as long as two days, sometimes in a refrigerator. It can be drunk cold or hot. In hot brewing, ground coffee is mixed with boiling or near-boiling water and steeped at most for a few minutes. In either case, the coffee grounds are sometimes pressed, and the beverage is then filtered to remove the grounds.

The researchers, who are at Thomas Jefferson University, previously assessed acidity and antioxidants in hot- and cold-brew java from lightly roasted beans. In another project, they analyzed the effect of medium and dark roasting, and of medium and coarse grinding, on a few coffee attributes. Now they're taking a deeper dive by roasting Columbian beans at five different temperatures -- from light roasting at 174 C to dark roasting at 209 C -- and studying the impact on acidity, antioxidants and other natural chemicals in hot- and cold-brew coffee.

With the lighter roasts, Rao's team finds that the content of caffeine and antioxidants is pretty similar in hot and cold brews. However, significant differences show up with the darker roasts. "Hot brewing extracts more antioxidants from the grind than cold brew, and this difference increases with the degree of roasting," Rao says. So hot-brew of dark roasts produces a potentially healthier drink. Hot brew also contains more of certain kinds of acids and total dissolved solids. The pH is about the same for both hot and cold brews at equivalent roast, though both become less acidic as roasting temperature increases.

"My advice to consumers has always been to drink what they like," Rao says. "But if you want to craft a coffee beverage with antioxidants or acidity in mind, you may want to pay attention to roast. If you want a low-acid drink, you may want to use a darker roast. But remember that the gap between the antioxidant content of hot- and cold-brew coffee is much larger for a darker roast."

One of the biggest challenges in all these projects is reproducibility. "We have performed experiments in which we're using the same beans, the same machine, the same settings, and it comes out not quite the same as the previous batch," Rao says. "The same goes for brewing. To create a cup that's consistent every time is really difficult. I have a lot of respect for the baristas who can do that." To approach that level of perfection, the researchers became like drill sergeants, carefully standardizing their processes. They developed a procedure for when the water should be added to the ground coffee, how to pour the water and for how long, how to shake the solution, how to press the brewed coffee and how to analyze it. They set time limits for each step, with margins of just a few seconds. This rigor produced much more reproducible results.

The team has now begun comparing the impact of the two brewing processes and degree of roast on furans, flavor compounds present in raw coffee beans that are also generated through roasting. Such compounds are particularly important in cold-brew joe, which doesn't have the same distinct aroma as the hot drink because it lacks steam to carry volatile organic compounds to the nose.
The researchers acknowledge support and funding from the U.S. Department of Agriculture's National Institute of Food and Agriculture.

The American Chemical Society (ACS) is a nonprofit organization chartered by the U.S. Congress. ACS' mission is to advance the broader chemistry enterprise and its practitioners for the benefit of Earth and its people. The Society is a global leader in providing access to chemistry-related information and research through its multiple research solutions, peer-reviewed journals, scientific conferences, eBooks and weekly news periodical Chemical & Engineering News. ACS journals are among the most cited, most trusted and most read within the scientific literature; however, ACS itself does not conduct chemical research. As a specialist in scientific information solutions (including SciFinder® and STN®), its CAS division powers global research, discovery and innovation. ACS' main offices are in Washington, D.C., and Columbus, Ohio.

To automatically receive news releases from the American Chemical Society, contact

Follow us on Twitter | Facebook


Role of roast on chemical characteristics of cold brew coffee


Hot and cold brew coffee samples brewed using Columbian coffee beans roasted at five different roast temperatures (174°C, 183°C, 194°C, 203°C, and 209°C) were analyzed. Caffeine concentration, total caffeoylquinic acid (CQA) concentration, total antioxidant activity, pH, total titratable acids, and total dissolved solids (TDS) were evaluated. Caffeine concentration ranged from 943.21 mg/L to 1055.60 mg/L and from 417.67 mg/L to 1147.59 mg/L for hot and cold samples respectively. Total CQA concentration ranged from 291.30 mg/L to 3213.21 mg/L and from 311.95 mg/L to 1648.47 mg/L for hot and cold samples respectively. In general, the hot brew method yielded higher caffeine and total CQA concentration than the cold brew method. Hot brew coffee samples exhibited a higher antioxidant capacity over cold brew coffee samples using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) decolorization assay. Coffee pH increased as roast temperature increased. At the same roast level, the pH between hot brew and cold brew coffee samples were comparable. The hot brew coffee samples were also found to have higher concentration of total titratable acids. Total dissolved solids (TDS) was observed to be greater in hot brew coffee samples than in cold brew counterparts. Increases in roasting temperature also led to an increase in TDS across coffee samples for both hot and cold brewing methods, with the highest TDS present in hot brew coffee samples roasted at 209°C. It is noted that with the exception of pH, all attributes of hot brew coffee samples have a direct relationship with roasting temperature, while increasing roast temperature exhibited an inverted u-shaped trend in the cold brew coffee samples. The results suggest that the bean roasting temperature significant impacts the chemistry of the final coffee brew and should not be ignored when crafting cold brew coffee beverages.

American Chemical Society

Related Coffee Articles:

A coffee and catnap keep you sharp on the nightshift
A simple coffee and a quick catnap could be the cure for staying alert on the nightshift as new research from the University of South Australia shows that this unlikely combination can improve attention and reduce sleep inertia.
Latest findings on bitter substances in coffee
Coffee is very popular around the world despite or perhaps because of its bitter taste.
Coffee linked to lower body fat in women
Women who drink two or three cups of coffee a day have been found to have lower total body and abdominal fat than those who drink less, according to a new study published in The Journal of Nutrition.
How to make the healthiest coffee during COVID-19 lockdown
We may all be drinking more coffee to help us survive the COVID-19 lockdown.
Coffee changes our sense of taste
Sweet food is even sweeter when you drink coffee. This is shown by the result of research from Aarhus University.
'Whiskey webs' are the new 'coffee ring effect'
Spilled coffee forms a ring as the liquid evaporates, depositing solids along the edge of the puddle.
Is your coffee contributing to malaria risk?
Researchers at the University of Sydney and University of São Paulo, Brazil, estimate 20% of the malaria risk in deforestation hot spots is driven by the international trade of exports including: coffee, timber, soybean, cocoa, wood products, palm oil, tobacco, beef and cotton.
The complex biology behind your love (or hatred) of coffee
Why do some people feel like they need three cups of coffee just to get through the day when others are happy with only one?
Room for complexity? The many players in the coffee agroecosystem
The BioScience Talks podcast features discussions of topical issues related to the biological sciences.
Coffee may protect against gallstones
Drinking more coffee may help reduce the risk of developing gallstones, according to a new study published in the Journal of Internal Medicine.
More Coffee News and Coffee Current Events

Trending Science News

Current Coronavirus (COVID-19) News

Top Science Podcasts

We have hand picked the top science podcasts of 2020.
Now Playing: TED Radio Hour

Debbie Millman: Designing Our Lives
From prehistoric cave art to today's social media feeds, to design is to be human. This hour, designer Debbie Millman guides us through a world made and remade–and helps us design our own paths.
Now Playing: Science for the People

#574 State of the Heart
This week we focus on heart disease, heart failure, what blood pressure is and why it's bad when it's high. Host Rachelle Saunders talks with physician, clinical researcher, and writer Haider Warraich about his book "State of the Heart: Exploring the History, Science, and Future of Cardiac Disease" and the ails of our hearts.
Now Playing: Radiolab

Insomnia Line
Coronasomnia is a not-so-surprising side-effect of the global pandemic. More and more of us are having trouble falling asleep. We wanted to find a way to get inside that nighttime world, to see why people are awake and what they are thinking about. So what'd Radiolab decide to do?  Open up the phone lines and talk to you. We created an insomnia hotline and on this week's experimental episode, we stayed up all night, taking hundreds of calls, spilling secrets, and at long last, watching the sunrise peek through.   This episode was produced by Lulu Miller with Rachael Cusick, Tracie Hunte, Tobin Low, Sarah Qari, Molly Webster, Pat Walters, Shima Oliaee, and Jonny Moens. Want more Radiolab in your life? Sign up for our newsletter! We share our latest favorites: articles, tv shows, funny Youtube videos, chocolate chip cookie recipes, and more. Support Radiolab by becoming a member today at