Nav: Home

Defects in epithelial tissue organization -- A question of life or death

April 13, 2017

Researchers from the Mechanobiology Institute, Singapore (MBI) at the National University of Singapore have discovered the primary mechanism driving the extrusion of dying cells from epithelial monolayers. This work was published in Nature on 13 April 2017.

Misalignment of cells predicts cell death and removal in epithelial tissue

The removal of cells from a tissue occurs regularly. Not only are damaged or dying cells removed, but the process of cell extrusion can prevent regions from becoming overcrowded. This is particularly important not only during developmental processes when tissues and organs are being formed, but also in diseases such as cancer, when tumors grow uncontrollably. Despite the importance of cell extrusion in development and aging, as well as the pathological importance in cancer progression, the cues that flag a cell for removal were poorly understood.

Now, by studying single-layers of epithelial cells grown in the lab, scientists from MBI and the Institut Jacques Monod, Centre national de la recherche scientifique (CNRS) and University Paris Diderot in collaboration with researchers from Oxford University and Institut Curie have found that the major factor driving cell death and removal relies on the physical arrangement of cells in the surrounding cell layer. In particular, the appearance of defects in the cellular patterns of epithelial layers promotes cell death and elimination from the tissues.

There are several examples in nature where a molecule or cell type aligns in a defined manner. Bacteria colonies, fat molecules, and even internal components of the cell, are just a few examples. Another well-known example corresponds to liquid crystals, a state of matter between a solid and a liquid, which can consist of rod-shaped molecules. Under certain conditions, these molecules can align along a preferential orientation when altered by electric, magnetic fields or temperature. This phenomenon is particularly well-known since it is exploited in technologies such as liquid crystal displays. In this case the optical properties of liquid crystals are determined by their alignment. Shifts in their alignment determine what we see on the display. As in any crystal, a perfect arrangement does not exist in liquid crystals and defects emerge in their arrangement that strongly modify their physical properties. The starting point of this study was to show how the behaviour of the cell sheet is analogous to liquid crystals.

Here, PhD candidate Mr Thuan Beng Saw, together with Prof Chwee Teck Lim of MBI, and Professor Benoit Ladoux of Institut Jacques Monod (IJM, CNRS) and MBI, in collaboration with Dr Amin Doostmohammadi and Professor Julia M. Yeomans (Oxford), Professor Philippe Marcq (Curie Institute) and Assistant Professor Yusuke Toyama (MBI), found that like the liquid crystals in a phone or laptop monitor, epithelial cells were arranged parallel to each other with their 'long' sides all facing the same direction. Following this analogy, they also observed the emergence of 'topological defects', which caused the cells to realign so that they resembled a comet. In this case, cells at the head of the comet pattern had shifted so that they now aligned perpendicular to the cells that made up the tail. Some cells turned up to 90 degrees. In a liquid crystal display, such realignments of the molecules merely alter the optical properties of the material. However, in an epithelial sheet, such changes in the pattern can mean life or death for the cells involved. Remarkably, it was after this cell realignment that cells near the head of the 'comet pattern' died and were removed from the surrounding tissue.

To further investigate the relationship between cell death and topological defects, they examined the forces being generated around these particular areas of cell misalignment. They found that compressive force concentrated at the head of the comet pattern. This force generated over an hour prior to cell extrusion, and was sufficient to trigger cell death at topological defects. As cells are connected to each other by protein cables and adhesions structures, any movement of a cell causes tension to be propagated to its neighbours. The misalignment of cells causes significant bending of cells and this leads to high compressive stresses in these regions. These stresses are sufficient to trigger apoptosis and cell extrusion of a nearby cell.

Tissue engineering and regenerative medicine requires scientists to carefully control cell growth and tissue development in a lab. The findings presented in this work are of fundamental importance towards achieving this control. Indeed, the researchers successfully controlled how cells aligned by introducing shapes in areas where the cells grew that mimicked the topological defects associated with cell death and extrusion. This allowed them to pinpoint where in the cell sheet extrusion would occur. These discoveries provide a significant step forward in our understanding of how the physical microenvironment plays a role in tissue development, and provides new approaches with which researchers can control, analyse and study cell growth and death.

National University of Singapore

Related Cell Death Articles:

Cell death or cancer growth: A question of cohesion
Activation of CD95, a receptor found on all cancer cells, triggers programmed cell death -- or does the opposite, namely stimulates cancer cell growth.
Cell death blocker prevents healthy cells from dying
Researchers have discovered a proof-of-concept drug that can prevent healthy cells from dying in the laboratory.
Road to cell death mapped in the Alzheimer's brain
Scientists have identified a new mechanism that accelerates aging in the brain and gives rise to the most devastating biological features of Alzheimer's disease.
Preventing cell death as novel therapeutic strategy for rheumatoid arthritis
A collaborative study by research groups from the University of Cologne, VIB, Ghent University, the Βiomedical Sciences Research Center 'Alexander Fleming' in Athens and the University of Tokyo identified a new molecular mechanism causing rheumatoid arthritis.
Atherosclerosis: Induced cell death destabilizes plaques
Many chronic disorders arise from misdirected immune responses. A Ludwig-Maximilians-Universitaet (LMU) in Munich team led by Oliver Söhnlein now shows that neutrophils exacerbate atherosclerosis by inducing smooth muscle-cell death and that a tailored peptide inhibits the process.
Cell death may be triggered by 'hit-and-run' interaction
A 'hit-and-run' interaction between two proteins could be an important trigger for cell death, according to new research from Walter and Eliza Hall Institute researchers.
How a mitochondrial enzyme can trigger cell death
Cytochrome c is a small enzyme that plays an important role in the production of energy by mitochondria.
Tuberculosis: Inhibiting host cell death with immunotherapy
DZIF scientists from the University Hospital Cologne are working on an immunotherapy that supports antibiotic treatment of tuberculosis.
Fine-tuning cell death: New component of death machinery revealed
An important component of the microscopic machinery that drives cell death has been identified by Australian scientists.
Road to cell death more clearly identified for Parkinson's disease
In experiments performed in mice, Johns Hopkins researchers report they have identified the cascade of cell death events leading to the physical and intellectual degeneration associated with Parkinson's disease.
More Cell Death News and Cell Death Current Events

Top Science Podcasts

We have hand picked the top science podcasts of 2019.
Now Playing: TED Radio Hour

In & Out Of Love
We think of love as a mysterious, unknowable force. Something that happens to us. But what if we could control it? This hour, TED speakers on whether we can decide to fall in — and out of — love. Guests include writer Mandy Len Catron, biological anthropologist Helen Fisher, musician Dessa, One Love CEO Katie Hood, and psychologist Guy Winch.
Now Playing: Science for the People

#543 Give a Nerd a Gift
Yup, you guessed it... it's Science for the People's annual holiday episode that helps you figure out what sciency books and gifts to get that special nerd on your list. Or maybe you're looking to build up your reading list for the holiday break and a geeky Christmas sweater to wear to an upcoming party. Returning are pop-science power-readers John Dupuis and Joanne Manaster to dish on the best science books they read this past year. And Rachelle Saunders and Bethany Brookshire squee in delight over some truly delightful science-themed non-book objects for those whose bookshelves are already full. Since...
Now Playing: Radiolab

An Announcement from Radiolab