Researchers describe role of novel mutations in fosfomycin resistance

April 22, 2018

Madrid, Spain: Researchers identified novel chromosomal mutations and described their role in the development of resistance of Escherichia coli (E. coli) to broad-spectrum antibiotic fosfomycin, according to research presented at the 28th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) [1].

Researchers from France studied the genetic basis of fosfomycin resistance in a panel of E. coli isolates and found that certain mutations rendered fosfomycin ineffective at lower doses compared with other mutations. They obtained four mutants in vitro and used a set of 20 clinical isolates, 11 of which were susceptible to antibiotics and nine of which were resistant. The team analysed the minimum inhibitory concentration (MIC) of fosfomycin, which is the concentration at which bacterial growth was prevented. A low MIC means that a smaller amount of the antibiotic was needed to stop growth compared to samples with high MICs.

"In this study, we have identified novel chromosomal mutations both selected in vitro and in vivo and experimentally determined their role in fosfomycin resistance," explained presenting author Prof. Vincent Cattoir. "Mutations in uhpB and uhpC appear to be more frequent than those in already known genes."

Researchers found no genetic mutations in the 11 E. coli isolates that responded to fosfomycin, with MICs ranging from 0.5 to 8 mg/L. However, they found several mutations in each of the nine fosfomycin-resistant isolates, which exhibited MICs in the range of 64-256 mg/L.

Cattoir's team obtained two mutants that corresponded with mutations in two novel genes, uhpB and uhpC. Additional mutations were noted on genes galU and Ion. When researchers introduced the uhpB and uhpC mutations, the amount of fosfomycin needed to stop the visible growth of E. coli was 64-fold. Single mutations in the galU and Ion genes only caused a two-fold increase in the MIC. Three other uhpB/uhpC mutations each led to a 128-fold increase in fosfomycin MICs.

Fosfomycin is an antibiotic used to treat bladder and urinary tract infections. Fosfomycin resistance results from a set of known chromosomal mutations or the acquisition of mutated genes from elsewhere, such as other bacterial species. But resistance is also observed in some strains that do not have these known mutations or acquired genes.
-end-
Paper Poster no: P1625, Novel mechanisms of fosfomycin resistance in Escherichia coli, session Fosfomycin resistance: So many news!, 1:30 - 2:30, Monday, 23 April 2018, Paper Poster Area

[1] The European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) is the annual meeting of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID). This year it will take place from 21 - 24 April 2018 in Madrid, Spain. At the world's largest congress combining the fields of infectious diseases and clinical microbiology, researchers will present more than 3,000 regular and late-breaking abstracts with the latest findings and recommendations, which are set to help improve diagnosis, prevention and treatment of infection-related diseases. The congress offers almost 200 sessions, including keynote lectures, symposia, oral sessions, educational workshops and meet-the-expert session. ECCMID expects approximately 13,000 participants from more than 100 countries.

About ESCMID

The European Society of Clinical Microbiology and Infectious Diseases (ESCMID) is a non-profit organization dedicated to improving the diagnosis, treatment and prevention of infectious diseases in Europe and beyond. The society promotes and supports research, education, training and good medical practice in infection-related disciplines with a special focus on antimicrobial resistance to build capacity throughout the world. http://www.escmid.org

Contact

Chantal Britt
ESCMID Communications Manager
ESCMID Executive Office
P.O. Box 214, CH-4010 Basel
Phone +41 61 508 01 57
Mobile +41 76 588 08 24
Email chantal.britt@escmid.org
http://www.escmid.org

Tara Giroud
ECCMID Communications Assistant
Mobile +41 78 705 79 85
Email taragiroud@gmail.com

European Society of Clinical Microbiology and Infectious Diseases

Related Infectious Diseases Articles from Brightsurf:

Understanding the spread of infectious diseases
Physicists at M√ľnster University (Germany) have shown in model simulations that the COVID-19 infection rates decrease significantly through social distancing.

Forecasting elections with a model of infectious diseases
Election forecasting is an innately challenging endeavor, with results that can be difficult to interpret and may leave many questions unanswered after close races unfold.

COVID-19 a reminder of the challenge of emerging infectious diseases
The emergence and rapid increase in cases of coronavirus disease 2019 (COVID-19), a respiratory illness caused by a novel coronavirus, pose complex challenges to the global public health, research and medical communities, write federal scientists from NIH's National Institute of Allergy and Infectious Diseases (NIAID) and from the Centers for Disease Control and Prevention (CDC).

Certain antidepressants could provide treatment for multiple infectious diseases
Some antidepressants could potentially be used to treat a wide range of diseases caused by bacteria living within cells, according to work by researchers in the Virginia Commonwealth University School of Medicine and collaborators at other institutions.

Opioid epidemic is increasing rates of some infectious diseases
The US faces a public health crisis as the opioid epidemic fuels growing rates of certain infectious diseases, including HIV/AIDS, hepatitis, heart infections, and skin and soft tissue infections.

Infectious diseases could be diagnosed with smartphones in sub-Saharan Africa
A new Imperial-led review has outlined how health workers could use existing phones to predict and curb the spread of infectious diseases.

The Lancet Infectious Diseases: Experts warn of a surge in vector-borne diseases as humanitarian crisis in Venezuela worsens
The ongoing humanitarian crisis in Venezuela is accelerating the re-emergence of vector-borne diseases such as malaria, Chagas disease, dengue, and Zika virus, and threatens to jeopardize public health gains in the country over the past two decades, warn leading public health experts.

Glow-in-the-dark paper as a rapid test for infectious diseases
Researchers from Eindhoven University of Technology (The Netherlands) and Keio University (Japan) present a practicable and reliable way to test for infectious diseases.

Math shows how human behavior spreads infectious diseases
Mathematics can help public health workers better understand and influence human behaviors that lead to the spread of infectious disease, according to a study from the University of Waterloo.

Many Americans say infectious and emerging diseases in other countries will threaten the US
An overwhelming majority of Americans (95%) think infectious and emerging diseases facing other countries will pose a 'major' or 'minor' threat to the U.S. in the next few years, but more than half (61%) say they are confident the federal government can prevent a major infectious disease outbreak in the US, according to a new national public opinion survey commissioned by Research!America and the American Society for Microbiology.

Read More: Infectious Diseases News and Infectious Diseases Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.