Evaluation of induced membrane vesicles fusion specificity with target cellsApril 23, 2018
Extracellular vesicles (EV) represent a promising vector system for biomolecules and drug delivery due to their natural origin and participation in intercellular communication. As the quantity of EVs is limited, it was proposed to induce the release of membrane vesicles from the surface of human cells by treatment with cytochalasin B. Cytochalasin B-induced membrane vesicles (CIMVs) were successfully tested as a vector for delivery of dye, nanoparticles, and a chemotherapeutic.
As team leader Marina Gomzikova explains, "The understanding of interaction between cytochalasin B-induced microvesicles (CIMVs) and target cells can help create specific vectors for anti-tumor drug delivery. This will allow for the decrease of toxic effect on normal cells and the enhancement of therapeutic results."
In this new research, the authors conducted a study of specificity of vesicle fusion with target cells. They evaluated the contribution of endocytosis, i. e. active transport of molecules into cells via the folding of plasma membrane. It was found out that proteinase K treatment effectively inhibited the internalization of induced membrane vesicles into target cells. Inhibition of interaction of membrane proteins of CIMVS with surface proteins of target cells is the biggest influence on the effectiveness of internalization of CIMVs.
Professor Albert Rizvanov, Head of Gene and Cell Technologies Lab, adds that this particular research is a very good example of an inquiry into biomimetic technology. In this case, it's the introduction of artificial microvesicles which are very similar to natural ones.
As the research has shown so far, CIMVs can become the basis for a new class of effective and safe anti-tumor medications.
Kazan Federal University
Related Human Cells Articles:
A research team, led by South Korea's Ulsan National Institute of Science and Technology has confirmed that targeting SnCs could treat age-related degenerative joint disease.
Boston University scientists have announced two major findings that further our understanding of how stem cells become organs: the ability to grow and purify the earliest lung progenitors that emerge from human stem cells, and the ability to differentiate these cells into tiny 'bronchospheres' that model cystic fibrosis.
Swedish researchers at Chalmers University of Technology and Sahlgrenska Academy have successfully induced human cartilage cells to live and grow in an animal model, using 3-D bioprinting.
Researchers have harvested genes from bacteria that, with a few tweaks, can create and enhance electrical signaling in cultured human cells.
MIT biological engineers have devised a way to record complex histories in the DNA of human cells, allowing them to store and retrieve memories of past events.
Evidence of grid cell activity has been seen in healthy volunteers asked to imagine moving through an environment.
Human stem cells that are capable of becoming any other kind of cell in the body have previously only been acquired and cultivated with difficulty.
Scientists at the Gladstone Institutes and the University of California, San Francisco have successfully converted human skin cells into fully-functional pancreatic cells.
Su-Chun Zhang, a pioneer in developing neurons from stem cells at the University of Wisconsin-Madison, has created a specialized nerve cell that makes serotonin, a signaling chemical with a broad role in the brain.
In new research appearing in the prestigious journal Nature Biotechnology, an international research team led by The Hebrew University of Jerusalem describes a new technique for growing human hepatocytes in the laboratory.
Related Human Cells Reading:
The Morphology of Human Blood Cells
by Ann Bell (Author)
This atlas, which portrays the morphologic characteristics of normal and pathologic cells in blood and bone marrow, is published for the use of medical students, student medical technologists, veterinary students, and other health science students who are learning to identify the various types of blood cells. This monograph also is an aid for teachers of morphological hematology and for technologists who are responsible for the examination of smears by manual or automated methods. A knowledge of morphology is also useful for residents in clinical and anatomic pathology, pediatrics, and... View Details
Human Physiology: From Cells to Systems (MindTap Course List)
by Lauralee Sherwood (Author)
NOTE: Access Code is NOT INCLUDED
Organized around the central theme of homeostasis--how the body meets changing demands while maintaining the internal constancy necessary for all cells and organs to function--HUMAN PHYSIOLOGY helps you appreciate the integrated functioning of the human body. Author Lauralee Sherwood uses clear, straightforward language, analogies, and frequent references to everyday experiences to help you learn and relate to physiology concepts. The vibrant art program and empowering digital resources--including robust 3D animations and rich... View Details
Cells (Science Readers: Content and Literacy)
by Teacher Created Materials (Author)
The human body is made of up trillions of cells! Without cells and their remarkable abilities, there would be no life. Layer by layer, you’ll journey into animal and plant cells to discover what these building blocks of life look like and how they function in this fact-filled life science book. From prokaryotes to eukaryotes, fifth-grade readers will learn all about cell theory, cell function, mitosis and meiosis, organelles in plant and animal cells, and more through this high-interest informational text filled with vibrant photographs. Aligned to the Next Generation Science Standards, a... View Details
Human Evolutionary Genetics
by Mark Jobling (Author), Edward Hollox (Author), Toomas Kivisild (Author), Chris Tyler-Smith (Author)
Now in full-color, the Second Edition of Human Evolutionary Genetics has been completely revised to cover the rapid advances in the field since publication of the highly regarded First Edition. Written for upper-level undergraduate and graduate students, it is the only textbook to integrate genetic, archaeological, and linguistic perspectives on human evolution, and to offer a genomic perspective, reflecting the shift from studies of specific regions of the genome towards comprehensive genomewide analyses of human genetic diversity.
Human Evolutionary Genetics is... View Details
Architecture of Human Living Fascia: Cells and Extracellular Matrix as Revealed by Endoscopy (Book & DVD)
by Jean-Claude Guimberteau (Author), Colin Armstrong (Author)
This unique book illustrates the structure of the fascia in the living human being. Dr Guimberteau's photographs provide a detailed account of fascial architecture. The accompanying text explains what the photographs mean, clarifies the importance of the fascia, and sets out the implications of these findings for everyday therapeutic practice. View Details
The Cell: Discovering the Microscopic World that Determines Our Health, Our Consciousness, and Our Future
by Joshua Z. Rappoport (Author)
Your body has trillions of cells, and each one has the complexity and dynamism of a city. Your life, your thoughts, your diseases, and your health are all the function of cells.
But what do you really know about what goes on inside you?
The last time most people thought about cells in any detail was probably in high school or a college general biology class. But the field of cell biology has advanced incredibly rapidly in recent decades, and a great deal of what we may have learned in high school and college is no longer accurate or particularly relevant.
The... View Details
Human Cell Culture Protocols (Methods in Molecular Biology)
by Ragai R. Mitry (Editor), Robin D. Hughes (Editor)
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also... View Details
Inside Out Human Body: Explore the World's Most Amazing Machine-You!
by Luann Columbo (Author)
Discover the amazing human body, system by system and layer by layer, and the incredible things it is capable of with Inside Out Human Body. Comes with a real, 3-D model to help you learn faster!
What do you think is the most amazing machine in the universe? A superconductor? The modern computer? A rocket ship? Think again: It’s the human body! Astonishingly intricate and complex, your body grows, heals itself, and performs a mind-boggling number of complex functions all at the same time!
Inside Out: Human... View Details
Human Behavior: A Cell to Society Approach
by Michael G. Vaughn (Author), Matt DeLisi (Author), Holly C. Matto (Author)
A unique approach to human behavior
that integrates and interprets the latest research from cell to society
Incorporating principles and findings from molecular biology, neuroscience, and psychological and sociocultural sciences, Human Behavior employs a decidedly integrative biosocial, multiple-levels-of-influence approach. This approach allows students to appreciate the transactional forces shaping life course opportunities and challenges among diverse populations in the United States and around the world.
Human Behavior includes case studies, Spotlight topics, and... View Details