Enzyme treatment reduces alcohol-induced liver damage in mouse models

April 25, 2017

An intestinal enzyme previously shown to keep bacterial toxins from passing from the gastrointestinal system into the bloodstream may be able to prevent or reduce the liver damage caused by excess alcohol consumption. In their report that will appear in the journal Digestive Diseases and Sciences and has been published online, a Massachusetts General Hospital (MGH) research team describes how oral doses of intestinal alkaline phosphatase (IAP) prevented the development of fatty liver in mouse models of both binge drinking and chronic alcohol consumption. The study also provides the first evidence of an expanded role of the liver's stellate cells in alcoholic liver disease.

"Liver damage is one of the most devastating effects of excess alcohol consumption, and so blocking this process could save millions of lives lost to alcohol-related liver diseases such as cirrhosis and liver cancer," says Richard Hodin, MD, of the MGH Department of Surgery, the study's senior author. "Along with direct toxic effects on the liver itself, alcohol appears to damage the liver through its effects on the intestinal lining, allowing bacterial toxins from the gut to cross the barrier and reach the liver. Since we know that IAP works to maintain a healthy gut barrier by blocking passage of an important toxic molecule, we investigated its potential to protect the liver from alcohol-induce damage."

Previous research by Hodin's group revealed that IAP helps to maintain a healthy intestinal microbial population by blocking the damaging effects of lipopolysaccharide (LPS), a molecule responsible for the toxic effects of several species of bacteria, and that the enzyme's anti-LPS effects could prevent the development of metabolic syndrome - a constellation of symptoms including obesity, abnormal glucose and lipid metabolism, and fatty liver - in mice fed a high-fat diet. Since LPS is known to play a role in alcohol-induced liver inflammation and its levels are known to rise with alcohol consumption, the MGH team investigated whether oral IAP supplementation could prevent alcoholic liver disease both by detoxifying the LPS released by gut bacteria and by preventing its passage from the gut into the liver's blood supply.

The team conducted experiments in two mouse models of binge drinking - either one large dose or three large doses given at 12-hour intervals - and a model of chronic alcohol consumption - steady alcohol consumption for 10 days. The results indicated that giving IAP either before or at the same time as an alcohol dose reduced levels of the ALT enzyme, a common sign of liver damage; reduced the accumulation of fat in the liver, the first sign of alcoholic liver disease; and reduced the production of inflammatory factors.

While mice that did not receive the enzyme before or during an alcohol dose were found to have elevations in circulating LPS, decreased expression of the tight junction proteins that maintain the barrier function of the intestinal lining, and increased intestinal inflammation, IAP supplementation prevented those effects. Activation of the hepatic stellate cells, which recently have been shown to contribute to alcoholic fatty liver disease, was also prevented by pretreatment with IAP. Administering IAP after alcohol dosing had no protective effects.

"Hepatic stellate cells are considered to be the central player in causing liver fibrosis - scarring or cirrhosis - which is the common endpoint leading to death in most liver diseases," says co-author Michael Choi, MD, of the MGH Gastrointestinal Unit. "Our results suggest that activated hepatic stellate cells are involved in even earlier stages of alcoholic liver disease and that activation can be prevented by pretreatment with IAP. Along with following up this study with human trials of IAP's protective effects, we also would like to know which gut bacterial components besides LPS are important in inducing liver inflammation, to understand more deeply the role of hepatic stellate cells in liver disease and to find ways to block their activation."
-end-
The co-lead authors of the Digestive Diseases and Sciences paper are Sulaiman Hamarneh, MD, MGH Department of Surgery; and Byeong-Moo Kim, PhD, MGH Gastrointestinal Unit. The study was supported by National Institutes of Health grants T32 DK007754 and P30 DK040561 and by the Ellison Foundation.

Massachusetts General Hospital, founded in 1811, is the original and largest teaching hospital of Harvard Medical School. The MGH Research Institute conducts the largest hospital-based research program in the nation, with an annual research budget of more than $800 million and major research centers in HIV/AIDS, cardiovascular research, cancer, computational and integrative biology, cutaneous biology, genomic medicine, medical imaging, neurodegenerative disorders, regenerative medicine, reproductive biology, systems biology, photomedicine and transplantation biology. The MGH topped the 2015 Nature Index list of health care organizations publishing in leading scientific journals and earned the prestigious 2015 Foster G. McGaw Prize for Excellence in Community Service. In August 2016 the MGH was once again named to the Honor Roll in the U.S. News & World Report list of "America's Best Hospitals."

Massachusetts General Hospital

Related Alcohol Consumption Articles from Brightsurf:

Excessive alcohol consumption during the COVID-19 pandemic
The full impact of COVID-19 on alcohol use is not yet known, but rates have been rising during the first few months of the pandemic.

Alcohol consumption rises sharply during pandemic shutdown
Anecdotal information has suggested that people are buying and consuming more alcohol during the pandemic shutdown.

Associations of alcohol consumption, alcohol-induced passing out with risk of dementia
The risk of future dementia associated with overall alcohol consumption and alcohol-induced loss of consciousness in a population of current drinkers was examined in this observational study with more than 131,000 adults.

Sweet coolers a gateway to increased alcohol consumption
Sweetened alcoholic beverages can promote harmful alcohol consumption among teens, new University of Guelph research finds.

The influence of alcohol consumption among cohabitating partners
Research has linked a partner's or spouse's drinking with changes in alcohol-related behaviors, but few studies have considered only cohabiting relationships.

Does alcohol consumption have an effect on arthritis?
Several previous studies have demonstrated that moderate alcohol consumption is linked with less severe disease and better quality of life in patients with rheumatoid arthritis, but a new Arthritis Care & Research study suggests that this might not be because drinking alcohol is beneficial.

Is alcohol consumption more helpful than harmful? It depends on your age
Studies of health effects of alcohol consumption may underestimate the risks of imbibing, particularly for younger people, according to a new study in the Journal of Studies on Alcohol and Drugs.

Link between alcohol consumption and breast cancer ignored by women most at risk
Middle aged women in Australia aren't getting the message about the proven link between alcohol consumption and breast cancer, at a time when more are drinking while cancer rates in their age bracket are increasing, according to a new study.

How much is too much? Even moderate alcohol consumption is a risk factor for atrial fibrillation
Excessive alcohol consumption is an established risk factor for atrial fibrillation (AF), but what are the effects of moderate and mild consumption on AF?

Moderate consumption of alcohol is associated with fewer hospitalizations
A study of the Department of Epidemiology and Prevention of I.R.C.C.S.

Read More: Alcohol Consumption News and Alcohol Consumption Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.