Ribosome biogenesis gene DEF/UTP25 is essential for liver homeostasis and regeneration

April 28, 2020

Hepatocytes are responsible for diverse metabolic activities in a liver. Proper ribosome biogenesis is essential to sustain the function of hepatocytes. There are approximately 200 factors involved in ribosome biogenesis, however, few studies have focused on the role of these factors in maintaining liver homeostasis. The digestive organ expansion factor (def) gene encodes a nucleolar protein Def that participates in ribosome biogenesis. In addition, Def forms a complex with cysteine protease Calpain3 (Capn3) and recruits Capn3 to the nucleolus to cleave protein targets including the tumor suppressor p53 and ribosome biogenesis factor Mpp10. However, the function of Def has not been characterized in the mammalian digestive organs.

In this report, researchers show that conditional knockout of the mouse def gene in hepatocytes causes cell morphology abnormality and constant infiltration of inflammatory cells in the liver. As age increase, the def conditional knockout liver displays multiple tissue damage foci and biliary hyperplasia. Moreover, partial hepatectomy leads to sudden acute death to the def conditional knockout mice and this phenotype is rescued by intragastric injection of the anti-inflammation drug dexamethasone one day before hepatectomy. These results demonstrate that Def is essential for maintaining the liver homeostasis and liver regeneration capacity in mammals.
See the article:

Huang, W., Chen, F., Ma, Q., Xin, J., Li, J., Chen, J., Zhou, B., Chen, M., Li, J., and Peng, J. (2020). Ribosome biogenesis gene DEF/UTP25 is essential for liver homeostasis and regeneration. Sci China Life Sci 63, https://doi.org/10.1007/s11427-019-1635-2

Science China Press

Related Hepatocytes Articles from Brightsurf:

When malaria parasites trick liver cells to let themselves in
A new study led by Maria Manuel Mota, group leader at Instituto de Medicina Molecular, now shows that malaria parasites secrete the protein EXP2 that is required for their entry into hepatocytes.

Study shows main cell type in the liver has key role in defending against some viruses
Scientists at Scripps Research have uncovered an important disease-fighting role for cells called hepatocytes, which constitute most of the liver.

Pigs grow new liver in lymph nodes, study shows
Hepatocytes -- the chief functional cells of the liver - are natural regenerators, and the lymph nodes serve as a nurturing place where they can multiply.

Ribosome biogenesis gene DEF/UTP25 is essential for liver homeostasis and regeneration
Digestive-organ-expansion-factor (Def) is a nucleolar factor. Depletion of Def causes hypoplastic digestive organs in zebrafish.

CRISPR-HOT: A new tool to 'color' specific genes and cells
Researchers from the group of Hans Clevers at the Hubrecht Institute have developed a new genetic tool to label specific genes in human organoids, or mini organs.

Magnetic nanomaterials become an effective treatment against liver fibrosis
Fibrosis may affect different body organs. It develops as a reaction to long-time inflammation and is supposed to isolate the inflammation site from surrounding tissues.

Regeneration mechanism discovered in mice could provide target for drugs to combat chronic liver disease
Researchers at the University of Cambridge have uncovered a novel molecular mechanism that allows damaged adult liver cells to regenerate, paving the way for design of drugs to boost regeneration in conditions such as cirrhosis or other chronic liver diseases where regeneration is impaired.

How hepatitis B and delta viruses establish infection of liver cells
Princeton University researchers have developed a new, scalable cell culture system that allows for detailed investigation of how host cells respond to infection with hepatitis B (HBV) and delta virus (HDV).

Researchers identify human protein that aids development of malaria parasite
Researchers in Japan have discovered that the Plasmodium parasites responsible for malaria rely on a human liver cell protein for their development into a form capable of infecting red blood cells and causing disease.

How viable is your liver after you die?
In a paper to be published in a forthcoming issue of TECHNOLOGY, a group of researchers from Harvard Medical School have done a study on the viability of donated livers and its correlation with donor demographics.

Read More: Hepatocytes News and Hepatocytes Current Events
Brightsurf.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means for sites to earn advertising fees by advertising and linking to Amazon.com.